EMG1 18S rRNA pseudouridine methyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: EMG1
Official Full Name: 18S rRNA pseudouridine methyltransferase
Primary source: SGD:S000004176
Locus tag: YLR186W
See related: AllianceGenome:SGD:S000004176; FungiDB:YLR186W; VEuPathDB:YLR186W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: NEP1
Summary: Enables rRNA (pseudouridine) methyltransferase activity. Involved in rRNA processing. Located in cytoplasm and nucleus. Part of small-subunit processome. Used to study Bowen-Conradi syndrome. Human ortholog(s) of this gene implicated in Bowen-Conradi syndrome. Orthologous to human EMG1 (EMG1 N1-specific pseudouridine methyltransferase). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See EMG1 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (523632..524390)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Utp30 and Nep1 act together during pre-ribosomal complex formation.
Title: Genetic interactions of yeast NEP1 (EMG1), encoding an essential factor in ribosome biogenesis.
snR57 and Nep1 participate in a pre-rRNA conformational switch that allows the proper assembly of 40S ribosomal protein S19.
Title: Nop6, a component of 90S pre-ribosomal particles, is required for 40S ribosomal subunit biogenesis in Saccharomyces cerevisiae.
the identification of tma23 and nop6 mutations as recessive suppressors of a nep1(ts) mutant allele and the nep1 deletion as well
Title: Mutations in the nucleolar proteins Tma23 and Nop6 suppress the malfunction of the Nep1 protein.
Emg1 represents a new structural class in the superfamily of alpha/beta knot fold methyltransferases.
Title: The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases.
These results suggest that Nep1p binds adjacent to helix 47 of the 18S rRNA and possibly supports the association of Rps19p to pre-ribosomal particles.
Title: Genetic evidence for 18S rRNA binding and an Rps19p assembly function of yeast nucleolar protein Nep1p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables rRNA (pseudouridine) methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables rRNA (pseudouridine) methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables rRNA (pseudouridine) methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables rRNA (pseudouridine) methyltransferase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables rRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables rRNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in rRNA base methylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in rRNA base methylation	IEA Inferred from Electronic Annotation more info
involved_in rRNA base methylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA base methylation	ISO Inferred from Sequence Orthology more info	PubMed
involved_in rRNA processing	IEA Inferred from Electronic Annotation more info
involved_in rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosomal small subunit biogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ribosomal small subunit biogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of 90S preribosome	HDA more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear periphery	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ribonucleoprotein complex	IEA Inferred from Electronic Annotation more info
part_of small-subunit processome	IBA Inferred from Biological aspect of Ancestor more info
part_of small-subunit processome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 18S rRNA pseudouridine methyltransferase

NP_013287.1

EC 2.1.1.260

Methyltransferase for rRNA; methylates pseudouridine 18S rRNA residue 1191; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independent of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and equivalent yeast mutation affects Emg1p dimerization and localization but not methyltransferase activity; human EMG1 complements lethality of null and ts mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

523632..524390

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182073.1 → NP_013287.1 TPA: 18S rRNA pseudouridine methyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013287.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYI9, E9P8U2, Q06287

UniProtKB/TrEMBL

A7A176, B3RH77, B5VNA4, C7GIX3, C8ZDF7, G2WJ18, N1P6S0

Conserved Domains (1) summary

cd18088
Location:41 → 246

Nep1-like; 18S rRNA (pseudouridine(1248)-N1)-methyltransferase Nep1