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    LEU4 2-isopropylmalate synthase LEU4 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855619, updated on 9-Jun-2024

    Summary

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    Gene symbol
    LEU4
    Gene description
    2-isopropylmalate synthase LEU4
    Primary source
    FungiDB:YNL104C
    Locus tag
    YNL104C
    See related
    SGD:S000005048; AllianceGenome:SGD:S000005048; VEuPathDB:YNL104C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables 2-isopropylmalate synthase activity. Involved in leucine biosynthetic process. Located in mitochondrion. [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See LEU4 in Genome Data Viewer
    Location:
    chromosome: XIV
    Exon count:
    1
    Sequence:
    Chromosome: XIV; NC_001146.8 (424895..426754, complement)

    Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene YEATS domain-containing protein YAF9 Neighboring gene phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP52 Neighboring gene Met4p Neighboring gene DNA-directed DNA polymerase alpha catalytic subunit POL1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001146.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Influence of mutation in the regulatory domain of α-isopropylmalate synthase from Saccharomyces cerevisiae on its activity and feedback inhibition. Takagi H, et al. Biosci Biotechnol Biochem, 2022 May 24. PMID 35333283
    2. Characterization of a New Saccharomyces cerevisiae Isolated From Hibiscus Flower and Its Mutant With L-Leucine Accumulation for Awamori Brewing. Abe T, et al. Front Genet, 2019. PMID 31231421, Free PMC Article
    3. Asp578 in LEU4p is one of the key residues for leucine feedback inhibition release in sake yeast. Oba T, et al. Biosci Biotechnol Biochem, 2005 Jul. PMID 16041129
    4. Trifluoroleucine resistance as a dominant molecular marker in transformation of strains of Saccharomyces cerevisiae isolated from wine. Bendoni B, et al. FEMS Microbiol Lett, 1999 Nov 15. PMID 10556716
    5. Trifluoroleucine resistance and regulation of alpha-isopropyl malate synthase in Saccharomyces cerevisiae. Cavalieri D, et al. Mol Gen Genet, 1999 Feb. PMID 10071221

    See all (75) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Influence of mutation in the regulatory domain of alpha-isopropylmalate synthase from Saccharomyces cerevisiae on its activity and feedback inhibition.
      Title: Influence of mutation in the regulatory domain of α-isopropylmalate synthase from Saccharomyces cerevisiae on its activity and feedback inhibition.
    2. Mutation from Asp578 to Tyr in LEU4p is a novel change causing release from leucine feedback inhibition.
      Title: Asp578 in LEU4p is one of the key residues for leucine feedback inhibition release in sake yeast.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables 2-isopropylmalate synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 2-isopropylmalate synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 2-isopropylmalate synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in L-leucine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in L-leucine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in L-leucine biosynthetic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in L-leucine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion HDA PubMed 
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    2-isopropylmalate synthase LEU4
    NP_014295.1
    • Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001146.8 Reference assembly

      Range
      424895..426754 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182942.1 → NP_014295.1  TPA: 2-isopropylmalate synthase LEU4 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014295.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W175, P06208
      UniProtKB/TrEMBL
      A0A8H4BVQ2, B3LNW3, C8ZGC6, D5KUG5, N1NXG3
      Conserved Domains (1) summary
      TIGR00970
      Location:35 → 596
      leuA_yeast; 2-isopropylmalate synthase, yeast type
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P06208.1

    Gene LinkOut

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