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    crb2 DNA repair protein [ Schizosaccharomyces pombe (fission yeast) ]

    Gene ID: 2540992, updated on 14-Nov-2024

    Summary

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    Official Symbol
    crb2
    Official Full Name
    DNA repair protein
    Primary source
    PomBase:SPBC342.05
    Locus tag
    SPOM_SPBC342.05
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Schizosaccharomyces pombe (strain: 972h-)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
    Also known as
    rhp9
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    Genomic context

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    See crb2 in Genome Data Viewer
    Location:
    chromosome: II
    Exon count:
    1
    Sequence:
    Chromosome: II; NC_003423.3 (4210967..4213577)

    Chromosome II - NC_003423.3Genomic Context describing neighboring genes Neighboring gene tRNA-Asn Neighboring gene 5S ribosomal RNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene histone lysine acetyltransferase Rtt109 Neighboring gene ncRNA Neighboring gene 19S proteasome regulatory subunit Rpn12 Neighboring gene ribokinase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003423.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural and functional analysis of the Crb2-BRCT2 domain reveals distinct roles in checkpoint signaling and DNA damage repair. Kilkenny ML, et al. Genes Dev, 2008 Aug 1. PMID 18676809, Free PMC Article
    2. Di-methyl H4 lysine 20 targets the checkpoint protein Crb2 to sites of DNA damage. Greeson NT, et al. J Biol Chem, 2008 Nov 28. PMID 18826944, Free PMC Article
    3. Cdc2 phosphorylation of Crb2 is required for reestablishing cell cycle progression after the damage checkpoint. Esashi F, et al. Mol Cell, 1999 Aug. PMID 10488332
    4. The fission yeast Crb2/Chk1 pathway coordinates the DNA damage and spindle checkpoint in response to replication stress induced by topoisomerase I inhibitor. Collura A, et al. Mol Cell Biol, 2005 Sep. PMID 16107732, Free PMC Article
    5. Retention but not recruitment of Crb2 at double-strand breaks requires Rad1 and Rad3 complexes. Du LL, et al. Mol Cell Biol, 2003 Sep. PMID 12917337, Free PMC Article

    See all (59) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The authors find that the 53BP1 orthologue Crb2 and Rev7 specifically repress long-range resection through the RecQ helicase-dependent pathway, thereby preventing hyper-resection.
      Title: Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks.
    2. The data supports a model such that elevated Cdc2 activity delays the transition of Crb2 from its G1 to its G2 mode by blocking Srs2 DNA helicase and Casein Kinase 1 (Hhp1).
      Title: Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.
    3. These results suggest that the main role of Crb2 in DNA damage checkpoint signaling is recruiting Chk1 to sites of DNA lesions.
      Title: Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint.
    4. Findings argue that binding of the Crb2 BRCT repeats to pH2AX is critical for checkpoint activity and provide new insight into the mechanisms of chromatin-mediated genome stability.
      Title: Requirement for the phospho-H2AX binding module of Crb2 in double-strand break targeting and checkpoint activation.
    5. Data show that polar interactions of Crb2 serine-548 and lysine-619 with the phosphate group of phospho-H2A (gamma-H2A) are critical for Crb2 IRIF formation and checkpoint function.
      Title: BRCT domain interactions with phospho-histone H2A target Crb2 to chromatin at double-strand breaks and maintain the DNA damage checkpoint.
    6. Structural and functional analysis of the crb2 domain reveals distinct roles in checkpoint signaling and DNA damage repair.
      Title: Structural and functional analysis of the Crb2-BRCT2 domain reveals distinct roles in checkpoint signaling and DNA damage repair.
    7. Histone modifications cooperate in the same Crb2 recruitment pathway, which also requires the Tudor and BRCT motifs in Crb2.
      Title: Histone modification-dependent and -independent pathways for recruitment of checkpoint protein Crb2 to double-strand breaks.
    8. Full Crb2 activity requires phosphorylation of threonine-215 by Cdc2.
      Title: Cooperative control of Crb2 by ATM family and Cdc2 kinases is essential for the DNA damage checkpoint in fission yeast.
    9. When the Crb2 mutants experience damaged replication forks upon inhibition of the religation activity of topoisomerase I, the Chk1 DNA damage pathway induces sustained activation of the spindle checkpoint
      Title: The fission yeast Crb2/Chk1 pathway coordinates the DNA damage and spindle checkpoint in response to replication stress induced by topoisomerase I inhibitor.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by PomBase

    Function Evidence Code Pubs
    NOT enables H4K20me3 modified histone binding EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables chromatin-protein adaptor activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables histone binding EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables histone binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables methylated histone binding EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA damage checkpoint signaling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic DNA damage checkpoint signaling EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in mitotic DNA damage checkpoint signaling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic DNA replication checkpoint signaling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic G2 DNA damage checkpoint signaling IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mitotic G2 DNA damage checkpoint signaling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of double-strand break repair via homologous recombination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in chromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus HDA PubMed 
    is_active_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in site of double-strand break IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA repair protein

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003423.3 Reference assembly

      Range
      4210967..4213577
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001023768.3 → NP_596748.1  DNA repair protein [Schizosaccharomyces pombe]

      See identical proteins and their annotated locations for NP_596748.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P87074, Q09754
      Conserved Domains (1) summary
      smart00292
      Location:539 → 640
      BRCT; breast cancer carboxy-terminal domain
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

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