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    REA1 AAA family ATPase midasin [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850796, updated on 7-Jul-2024

    Summary

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    Gene symbol
    REA1
    Gene description
    AAA family ATPase midasin
    Primary source
    FungiDB:YLR106C
    Locus tag
    YLR106C
    See related
    SGD:S000004096; AllianceGenome:SGD:S000004096; VEuPathDB:YLR106C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    MDN1
    Summary
    Enables ATP hydrolysis activity. Involved in rRNA processing; regulation of ribosomal subunit export from nucleus; and ribonucleoprotein complex subunit organization. Located in nucleoplasm. Orthologous to human MDN1 (midasin AAA ATPase 1). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See REA1 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (349006..363738, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene tRNA splicing endonuclease subunit SEN2 Neighboring gene ncRNA Neighboring gene RNA exonuclease Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1. Mickolajczyk KJ, et al. Proc Natl Acad Sci U S A, 2020 Aug 4. PMID 32694211, Free PMC Article
    2. The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1. Sosnowski P, et al. Elife, 2018 Nov 26. PMID 30460895, Free PMC Article
    3. Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. Hocine S, et al. Nat Methods, 2013 Feb. PMID 23263691, Free PMC Article
    4. Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, et al. Science, 2011 Apr 22. PMID 21512033, Free PMC Article
    5. Expression and genomic analysis of midasin, a novel and highly conserved AAA protein distantly related to dynein. Garbarino JE, et al. BMC Genomics, 2002 Jul 8. PMID 12102729, Free PMC Article

    See all (89) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1.
      Title: Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1.
    2. The CryoEM molecular structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1 has been presented.
      Title: The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1.
    3. Release of Nug2 from pre-60S particles requires both its K(+)-dependent GTPase activity and the remodelling ATPase activity of Rea1.
      Title: Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export.
    4. Data suggest that Rea1 functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus.
      Title: The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly.
    5. Rea1 aligns with its interacting partners on the preribosome to effect a necessary step on the path to the export-competent 60S subunit.
      Title: Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits.
    6. Rea1 is a dynein-related nuclear AAA-ATPase is involved in late rRNA processing and nuclear export of 60 S subunits
      Title: Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in protein-RNA complex remodeling IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein-RNA complex remodeling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of ribosomal subunit export from nucleus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosomal large subunit assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ribosomal large subunit assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in ribosomal large subunit assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosomal large subunit export from nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in mitochondrion HDA PubMed 
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of preribosome, large subunit precursor IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    AAA family ATPase midasin
    NP_013207.1
    • Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex; involved with interaction partners Rsa4p and Ytm1p, in the ATP-dependent remodeling of the pre-60S particle at successive maturation steps during ribosomal biogenesis; involved in the removal of biogenesis factors including GTPase Nog2p prior to nuclear export; contains a hexameric AAA-motor head domain and a long flexible tail with a MIDAS (metal ion-dependent adhesion site) domain

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      349006..363738 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181993.1 → NP_013207.1  TPA: AAA family ATPase midasin [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013207.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VYA6, Q12019, Q7LGX0
      UniProtKB/TrEMBL
      A0A8H4BZH1, N1NY63
      Conserved Domains (1) summary
      COG5271
      Location:89 → 4910
      MDN1; Midasin, AAA ATPase with vWA domain, involved in ribosome maturation [Translation, ribosomal structure and biogenesis]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q12019.1

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