UBR2 putative ubiquitin-protein ligase UBR2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: UBR2
Official Full Name: putative ubiquitin-protein ligase UBR2
Primary source: SGD:S000004014
Locus tag: YLR024C
See related: AllianceGenome:SGD:S000004014; FungiDB:YLR024C; VEuPathDB:YLR024C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ubiquitin-protein transferase activity. Involved in cytoplasm protein quality control by the ubiquitin-proteasome system and protein polyubiquitination. Located in cytoplasm. Part of MUB1-RAD6-UBR2 ubiquitin ligase complex. Human ortholog(s) of this gene implicated in Johanson-Blizzard syndrome. Orthologous to several human genes including UBR1 (ubiquitin protein ligase E3 component n-recognin 1) and UBR2 (ubiquitin protein ligase E3 component n-recognin 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See UBR2 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (187663..193281, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Innate immunity to yeast prions: Btn2p and Cur1p curing of the [URE3] prion is prevented by 60S ribosomal protein deficiency or ubiquitin/proteasome system overactivity.
Title: Innate immunity to yeast prions: Btn2p and Cur1p curing of the [URE3] prion is prevented by 60S ribosomal protein deficiency or ubiquitin/proteasome system overactivity.
Altogether, these data indicate that the absence of R37 methylation on Cse4 reduces the recruitment of kinetochore proteins to centromeric chromatin, and that this can be compensated for by stabilizing the outer kinetochore protein Dsn1 via the ubiquitin ligase complex Ubr2/Mub1.
Title: Defects in methylation of arginine 37 on CENP-A/Cse4 are compensated by the ubiquitin ligase complex Ubr2/Mub1.
Psh1 and Ubr2 synergistically control the amount of proteins at the kinetochore.
Title: Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.
Although Mub1/Ubr2 are not stable kinetochore components in vivo, they regulate the levels of the conserved outer kinetochore protein Dsn1 via ubiquitylation
Title: The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein.
Data show that ubiquitin-protein ligases Ubr1 and Ubr2 have opposing roles in Ste11DeltaNK444R-GFP aggregation.
Title: A network of ubiquitin ligases is important for the dynamics of misfolded protein aggregates in yeast.
Data suggest that Ubr1 and Ubr2 represent E3 components of a novel quality control pathway for proteins synthesized on cytosolic ribosomes.
Title: Ubr1 and Ubr2 function in a quality control pathway for degradation of unfolded cytosolic proteins.
Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase
Title: Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to unfolded protein	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cytoplasm protein quality control by the ubiquitin-proteasome system	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process via the N-end rule pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process via the N-end rule pathway	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of MUB1-RAD6-UBR2 ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: putative ubiquitin-protein ligase UBR2

NP_013124.1

EC 2.3.2.27

Cytoplasmic ubiquitin-protein ligase (E3); component of the Mub1p-Ubr2p-Rad6p ubiquitin ligase complex required for the ubiquitination and degradation of Rpn4p; mediates formation of the ternary complex

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

187663..193281 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181911.2 → NP_013124.1 TPA: putative ubiquitin-protein ligase UBR2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013124.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VY26, Q07963

UniProtKB/TrEMBL

B5VMW3

Conserved Domains (3) summary

smart00396
Location:96 → 171

ZnF_UBR1; Putative zinc finger in N-recognin, a recognition component of the N-end rule pathway

pfam18995
Location:1434 → 1842

PRT6_C; Proteolysis_6 C-terminal

cl17238
Location:1314 → 1369

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain