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    CGI121 Cgi121p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854972, updated on 17-May-2024

    Summary

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    Gene symbol
    CGI121
    Gene description
    Cgi121p
    Primary source
    FungiDB:YML036W
    Locus tag
    YML036W
    See related
    SGD:S000004500; AllianceGenome:SGD:S000004500
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Involved in positive regulation of transcription by RNA polymerase II; regulation of cell aging; and telomere maintenance via recombination. Part of EKC/KEOPS complex. Human ortholog(s) of this gene implicated in Galloway-Mowat syndrome 5. Orthologous to human TPRKB (TP53RK binding protein). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See CGI121 in Genome Data Viewer
    Location:
    chromosome: XIII
    Exon count:
    2
    Sequence:
    Chromosome: XIII; NC_001145.3 (205642..206293)

    Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene Ymd8p Neighboring gene Lft1p Neighboring gene AMP deaminase Neighboring gene Src1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001145.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A substrate binding model for the KEOPS tRNA modifying complex. Beenstock J, et al. Nat Commun, 2020 Dec 4. PMID 33277478, Free PMC Article
    2. Structural and functional characterization of KEOPS dimerization by Pcc1 and its role in t6A biosynthesis. Wan LC, et al. Nucleic Acids Res, 2016 Aug 19. PMID 27302132, Free PMC Article
    3. Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex. Zhang W, et al. Nucleic Acids Res, 2015 Mar 31. PMID 25735745, Free PMC Article
    4. Telomere recombination preferentially occurs at short telomeres in telomerase-null type II survivors. Fu XH, et al. PLoS One, 2014. PMID 24594632, Free PMC Article
    5. Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system. Wan LC, et al. Nucleic Acids Res, 2013 Jul. PMID 23620299, Free PMC Article

    See all (43) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Reciprocal hemizygosity analysis reveals that the Saccharomyces cerevisiae CGI121 gene affects lag time duration in synthetic grape must.
      Title: Reciprocal hemizygosity analysis reveals that the Saccharomyces cerevisiae CGI121 gene affects lag time duration in synthetic grape must.
    2. Deletion of CGI121 in the short-lived yku80(tel) mutant restores lifespan to cgi121Delta level, supporting the function of Cgi121 in telomeric single-stranded DNA generation and thus in promotion of telomere recombination.
      Title: Inhibition of telomere recombination by inactivation of KEOPS subunit Cgi121 promotes cell longevity.
    3. the crystal structure of Bud32/Cgi121 in complex with ADP revealed that ADP is bound in the catalytic site of Bud32 in a canonical manner
      Title: Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex.
    4. EKC/KEOPS complex is specifically involved in the regulation of Bud9p localization downstream of Rax1p/Rax2p.
      Title: Cell polarity in Saccharomyces cerevisiae depends on proper localization of the Bud9 landmark protein by the EKC/KEOPS complex.
    5. Cgi121 is part of the conserved KEOPS protein complex which promotes both telomere uncapping and telomere elongation
      Title: A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables tRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA threonylcarbamoyladenosine modification IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in telomere maintenance IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in telomere maintenance via recombination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance via recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of EKC/KEOPS complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of EKC/KEOPS complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome, telomeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Cgi121p
    NP_013676.2
    • Component of the EKC/KEOPS complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; Cgi121p is dispensable for tRNA modification; other complex members are Bud32p, Kae1p, Pcc1p, and Gon7p; ortholog of human TPRKB

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001145.3 Reference assembly

      Range
      205642..206293
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182394.1 → NP_013676.2  TPA: Cgi121p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013676.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      A2TBM6, D6VZD9, Q03705, Q6Q5A2
      UniProtKB/TrEMBL
      C7GT65, C8ZEG9
      Conserved Domains (1) summary
      pfam08617
      Location:19 → 179
      CGI-121; Kinase binding protein CGI-121
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    4WW5_B.1
    Q03705.1

    Gene LinkOut

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