mre11 Mre11 nuclease [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: mre11
Official Full Name: Mre11 nuclease
Primary source: PomBase:SPAC13C5.07
Locus tag: SPOM_SPAC13C5.07
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: rad32
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Genomic context

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See mre11 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 5

Sequence:: Chromosome: I; NC_003424.3 (436179..439980)

Chromosome I - NC_003424.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003424.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ccq1 restrains Mre11-mediated degradation to distinguish short telomeres from double-strand breaks.
Title: Ccq1 restrains Mre11-mediated degradation to distinguish short telomeres from double-strand breaks.
A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity.
Title: A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity.
a mre11 mutant defective in endonucleolytic activity, the same mutant lacking Ctp1, or the triple mutant also lacking the putative hairpin nuclease Pso2 showed wild-type levels of repair. Thus, MRN (Mre11-Rad50-Nbs1)may act to recruit the hairpin opening activity that allows subsequent repair
Title: Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe.
Mre11-Rad50-Nbs1 participates in checkpoint-dependent replication slowing through negative regulation of recombination.
Title: The fission yeast Rad32(Mre11)-Rad50-Nbs1 complex acts both upstream and downstream of checkpoint signaling in the S-phase DNA damage checkpoint.
Rad32 nuclease has the catalytic site for Rec12-oligonucleotide generation and is activated by Ctp1, which plays an additional role in meiotic recombination.
Title: Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes.
Study finds that in the presence of the MRN (Rad32-Rad50-Nbs1) complex efficient recombination requires Ctp1, the ortholog of the nuclease Sae2, but not the nuclease activity of MRN.
Title: Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination.
The Mre11-Rad50-Nbs1 S-phase checkpoint role is separate from its Ctp1- and resection-dependent role in double-strand break repair.
Title: The role of MRN in the S-phase DNA damage checkpoint is independent of its Ctp1-dependent roles in double-strand break repair and checkpoint signaling.
This study demonstrates for the first time that Mre11 (Schizosaccharomyces pombe Rad32(Mre11)) nuclease activity is required for the removal of Rec12(Spo11).
Title: Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functions.
Schizosaccharomyces pombe Rad32(Mre11) nuclease activity is involved in the removal of both Top2 from 5' DNA ends as well as Top1 from 3' ends in vivo and a ctp1(CtIP) deletion is defective for Top2 removal but overproficient for Top1 removal.
Title: Distinct requirements for the Rad32(Mre11) nuclease and Ctp1(CtIP) in the removal of covalently bound topoisomerase I and II from DNA.
The conserved Mre11/Rad50/Nbs1 (MRN) complex is likely directly involved in processing palindromes through the homologous recombination pathway of DNA repair. [Mre11]
Title: A novel recombination pathway initiated by the Mre11/Rad50/Nbs1 complex eliminates palindromes during meiosis in Schizosaccharomyces pombe.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables DNA end binding	ISO Inferred from Sequence Orthology more info	PubMed
enables Y-form DNA binding	ISO Inferred from Sequence Orthology more info	PubMed
enables double-stranded DNA 3'-5' DNA exonuclease activity	ISO Inferred from Sequence Orthology more info	PubMed
enables manganese ion binding	IDA Inferred from Direct Assay more info	PubMed
enables nuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA 3'-5' DNA exonuclease activity	ISO Inferred from Sequence Orthology more info	PubMed
enables single-stranded DNA endodeoxyribonuclease activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair involved in meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via nonhomologous end joining	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic DNA double-strand break formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic G2 DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic intra-S DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of Mre11 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Mre11 complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in chromosome, telomeric repeat region	IDA Inferred from Direct Assay more info	PubMed
is_active_in site of double-strand break	IBA Inferred from Biological aspect of Ancestor more info
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Mre11 nuclease

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003424.3 Reference assembly

Range

436179..439980

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001018336.3 → NP_592935.1 Mre11 nuclease [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_592935.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

Q09683

Conserved Domains (3) summary

TIGR00583
Location:15 → 414

mre11; DNA repair protein (mre11)

cd00840
Location:19 → 280

MPP_Mre11_N; Mre11 nuclease, N-terminal metallophosphatase domain

pfam04152
Location:301 → 467

Mre11_DNA_bind; Mre11 DNA-binding presumed domain