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    DHX33 DEAH-box helicase 33 [ Homo sapiens (human) ]

    Gene ID: 56919, updated on 2-Nov-2024

    Summary

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    Official Symbol
    DHX33provided by HGNC
    Official Full Name
    DEAH-box helicase 33provided by HGNC
    Primary source
    HGNC:HGNC:16718
    See related
    Ensembl:ENSG00000005100 MIM:614405; AllianceGenome:HGNC:16718
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    DDX33
    Summary
    This gene encodes a member of the DEAD box protein family. The DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
    Expression
    Ubiquitous expression in brain (RPKM 5.2), appendix (RPKM 3.8) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See DHX33 in Genome Data Viewer
    Location:
    17p13.2
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 17 NC_000017.11 (5440917..5468982, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 17 NC_060941.1 (5334655..5362725, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (5344237..5372302, complement)

    Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene rabaptin, RAB GTPase binding effector protein 1 Neighboring gene nucleoporin 88 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr17:5316624-5317823 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr17:5322619-5323156 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:5323157-5323694 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8069 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11573 Neighboring gene ReSE screen-validated silencer GRCh37_chr17:5342450-5342632 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8070 Neighboring gene RPA interacting protein Neighboring gene complement C1q binding protein Neighboring gene H3K27ac hESC enhancer GRCh37_chr17:5371354-5371936 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11575 Neighboring gene DHX33 divergent transcript Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11576 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11577 Neighboring gene ReSE screen-validated silencer GRCh37_chr17:5389715-5389882 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 11578 Neighboring gene derlin 2 Neighboring gene MIS12 kinetochore complex component

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. RNA helicase DHX33 regulates HMGB family genes in human cancer cells. Wang X, et al. Cell Signal, 2023 Oct. PMID 37543097
    2. DHX33 promotes colon cancer development downstream of Wnt signaling. Zhu Y, et al. Gene, 2020 Apr 20. PMID 32004669
    3. DHX33 expression is increased in hepatocellular carcinoma and indicates poor prognosis. Tian QH, et al. Biochem Biophys Res Commun, 2016 May 13. PMID 27073163
    4. The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition. Wang H, et al. Cell Signal, 2019 Feb. PMID 30552990
    5. Comprehensive analysis of immune implication and prognostic value of DHX33 in sarcoma. Zhang X, et al. Medicine (Baltimore), 2023 Apr 25. PMID 37115050, Free PMC Article

    See all (76) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. RNA helicase DHX33 regulates HMGB family genes in human cancer cells.
      Title: RNA helicase DHX33 regulates HMGB family genes in human cancer cells.
    2. Systematic analysis of the prognostic and immunological role of DHX33 in pan-cancer.
      Title: Systematic analysis of the prognostic and immunological role of DHX33 in pan-cancer.
    3. Comprehensive analysis of immune implication and prognostic value of DHX33 in sarcoma.
      Title: Comprehensive analysis of immune implication and prognostic value of DHX33 in sarcoma.
    4. GSK-3beta phosphorylation of DHX33 leads to its ubiquitination mediated protein degradation.
      Title: GSK-3β phosphorylation of DHX33 leads to its ubiquitination mediated protein degradation.
    5. Long non-coding RNA HOTAIR promotes hepatocellular carcinoma progression by regulating miR-526b-3p/DHX33 axis.
      Title: Long non-coding RNA HOTAIR promotes hepatocellular carcinoma progression by regulating miR-526b-3p/DHX33 axis.
    6. Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
      Title: Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
    7. DHX33 Recruits Gadd45a To Cause DNA Demethylation and Regulates a Subset of Gene Transcription.
      Title: DHX33 Recruits Gadd45a To Cause DNA Demethylation and Regulates a Subset of Gene Transcription.
    8. This study highlights that DHX33 protein is critical in glioblastoma cell proliferation and its potential as a novel therapeutic drug target.
      Title: The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition.
    9. DHX33 is highly expressed in human colon cancers. DHX33 and Wnt/beta-catenin reciprocally regulate each other.
      Title: DHX33 promotes colon cancer development downstream of Wnt signaling.
    10. Results reveal that DHX33 represses apoptosis through the direct upregulation of Bcl-2 gene transcription. AP-2beta is a binding partner for DHX33 and that DHX33 acts as a coactivator for AP-2beta to promote the transcription of antiapoptotic Bcl-2 gene.
      Title: DHX33 Interacts with AP-2β To Regulate Bcl-2 Gene Expression and Promote Cancer Cell Survival.
    Submit: New GeneRIF Correction See all GeneRIFs (21)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Replication interactions

    Interaction Pubs
    Knockdown of DEAH (Asp-Glu-Ala-His) box polypeptide 33 (DHX33) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed

    Go to the HIV-1, Human Interaction Database

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • FLJ21972, DKFZp762F2011

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding HDA PubMed 
    enables RNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables double-stranded RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables mRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables rDNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribosomal large subunit binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in positive regulation of MAPK cascade ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in positive regulation of NF-kappaB transcription factor activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in positive regulation of NLRP3 inflammasome complex assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase I IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of transcription by RNA polymerase I IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of type I interferon production ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in translational initiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of NLRP3 inflammasome complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleolus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ATP-dependent RNA helicase DHX33
    Names
    DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 33
    DEAH (Asp-Glu-Ala-His) box polypeptide 33
    DEAH box protein 33
    putative ATP-dependent RNA helicase DHX33
    NP_001186628.1
    NP_064547.2
    XP_016880366.1
    XP_047292374.1
    XP_054172671.1
    XP_054172672.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001199699.2 → NP_001186628.1  ATP-dependent RNA helicase DHX33 isoform 2

      See identical proteins and their annotated locations for NP_001186628.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an exon in the 5' coding region and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus, compared to isoform 1.
      Source sequence(s)
      AC004148, AK295760, BC030017, BQ775963
      UniProtKB/TrEMBL
      I3L1L6
      Related
      ENSP00000458925.1, ENST00000572490.1
      Conserved Domains (5) summary
      smart00847
      Location:305 → 390
      HA2; Helicase associated domain (HA2) Add an annotation
      COG1643
      Location:1 → 522
      HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
      cd00046
      Location:1 → 61
      DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
      pfam00271
      Location:118 → 235
      Helicase_C; Helicase conserved C-terminal domain
      pfam07717
      Location:424 → 524
      OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold

    2. NM_020162.4 → NP_064547.2  ATP-dependent RNA helicase DHX33 isoform 1

      See identical proteins and their annotated locations for NP_064547.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AC004148, AK295760, BQ775963
      Consensus CDS
      CCDS11072.1
      UniProtKB/Swiss-Prot
      B4DHF9, Q4G149, Q5CZ73, Q9H5M9, Q9H6R0
      UniProtKB/TrEMBL
      B4DIS6
      Related
      ENSP00000225296.3, ENST00000225296.8
      Conserved Domains (5) summary
      smart00847
      Location:478 → 563
      HA2; Helicase associated domain (HA2) Add an annotation
      COG1643
      Location:67 → 695
      HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
      cd00046
      Location:92 → 234
      DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
      pfam00271
      Location:291 → 408
      Helicase_C; Helicase conserved C-terminal domain
      pfam07717
      Location:597 → 697
      OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000017.11 Reference GRCh38.p14 Primary Assembly

      Range
      5440917..5468982 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017024877.2 → XP_016880366.1  ATP-dependent RNA helicase DHX33 isoform X2

      UniProtKB/Swiss-Prot
      Q9H6R0
      Conserved Domains (1) summary
      COG1643
      Location:1 → 267
      HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]

    2. XM_047436418.1 → XP_047292374.1  ATP-dependent RNA helicase DHX33 isoform X1

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060941.1 Alternate T2T-CHM13v2.0

      Range
      5334655..5362725 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054316697.1 → XP_054172672.1  ATP-dependent RNA helicase DHX33 isoform X2

    2. XM_054316696.1 → XP_054172671.1  ATP-dependent RNA helicase DHX33 isoform X1

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9H6R0.2 GenPept UniProtKB/Swiss-Prot:Q9H6R0

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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