Prkaca protein kinase, cAMP dependent, catalytic, alpha [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkacaprovided by MGI
Official Full Name: protein kinase, cAMP dependent, catalytic, alphaprovided by MGI
Primary source: MGI:MGI:97592
See related: Ensembl:ENSMUSG00000005469 AllianceGenome:MGI:97592
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PKCD; Pkaca
Summary: This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Apr 2013]
Expression: Ubiquitous expression in heart adult (RPKM 86.7), subcutaneous fat pad adult (RPKM 59.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C2; 8 40.22 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (84699607..84723073)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (83972978..83996444)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Vascular and Liver Homeostasis in Juvenile Mice Require Endothelial Cyclic AMP-Dependent Protein Kinase A.
Title: Vascular and Liver Homeostasis in Juvenile Mice Require Endothelial Cyclic AMP-Dependent Protein Kinase A.
Adipocyte HIF2alpha functions as a thermostat via PKA Calpha regulation in beige adipocytes.
Title: Adipocyte HIF2α functions as a thermostat via PKA Cα regulation in beige adipocytes.
Pde8b haploinsufficiency in mice is associated with modest adrenal defects, impaired steroidogenesis, and male infertility, unaltered by concurrent PKA or Wnt activation.
Title: Pde8b haploinsufficiency in mice is associated with modest adrenal defects, impaired steroidogenesis, and male infertility, unaltered by concurrent PKA or Wnt activation.
Limiting Self-Renewal of the Basal Compartment by PKA Activation Induces Differentiation and Alters the Evolution of Mammary Tumors.
Title: Limiting Self-Renewal of the Basal Compartment by PKA Activation Induces Differentiation and Alters the Evolution of Mammary Tumors.
Cyclic AMP Regulates Key Features of Macrophages via PKA: Recruitment, Reprogramming and Efferocytosis.
Title: Cyclic AMP Regulates Key Features of Macrophages via PKA: Recruitment, Reprogramming and Efferocytosis.
Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cells.
Title: Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cells.
The study used protein kinase A (PKA) as the model system and perform microsecond range molecular dynamics (MD) simulations on six variants which differ from one another in terms of active and inactive form, with or without bound ligands, C-terminal tail and phosphorylation at the activation loop. The root mean square fluctuations in the MD simulations are generally higher for the inactive forms than the active forms.
Title: Long-range molecular dynamics show that inactive forms of Protein Kinase A are more dynamic than active forms.
The cardiac RyR2 phosphorylation hotspot embraces PKA in a phosphorylation-dependent manner.
Title: The Cardiac Ryanodine Receptor Phosphorylation Hotspot Embraces PKA in a Phosphorylation-Dependent Manner.
AKAP121/PKA confers neuroprotection in an in vitro model of oxidative stress induced by exposure to excess glutamate.
Title: Neuroprotective Mitochondrial Remodeling by AKAP121/PKA Protects HT22 Cell from Glutamate-Induced Oxidative Stress.
Role of protein kinase A on PP1 activation in the endothelial cells.nNOS is activated in endothelial cells through protein kinase A and subsequent PP1 activation.
Title: Modulation of nNOS(ser852) phosphorylation and translocation by PKA/PP1 pathway in endothelial cells.

Submit: New GeneRIF Correction See all GeneRIFs (179)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Prkaca (e-PCR), detects polymorphism
- UniSTS:507174

Prkaca (e-PCR), detects polymorphism
- UniSTS:144189

PMC18620P1 (e-PCR)
- UniSTS:271694

Prkaca (e-PCR), detects polymorphism
- UniSTS:547699

RH128078 (e-PCR)
- UniSTS:211386

Prkaca (e-PCR), detects polymorphism
- UniSTS:507857

RH94810 (e-PCR)
- UniSTS:120056

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables cAMP-dependent protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cAMP-dependent protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cAMP-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables cAMP-dependent protein kinase activity	TAS Traceable Author Statement more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables manganese ion binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase A regulatory subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase A regulatory subunit binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase A regulatory subunit binding	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	TAS Traceable Author Statement more info
enables protein serine/threonine/tyrosine kinase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in cAMP/PKA signal transduction	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within cellular response to cold	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to glucagon stimulus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to glucose stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to heat	ISO Inferred from Sequence Orthology more info
involved_in cellular response to heat	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within cellular response to parathyroid hormone stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mesoderm formation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of TORC1 signaling	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of glycolytic process through fructose-6-phosphate	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of glycolytic process through fructose-6-phosphate	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of meiotic cell cycle	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of smoothened signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within neural tube closure	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-serine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within peptidyl-threonine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cholesterol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gluconeogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of insulin secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of protein export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	EXP Inferred from Experiment more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_positive_effect protein export from nucleus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of protein export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_positive_effect protein export from nucleus	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within protein localization to lipid droplet	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of bicellular tight junction assembly	IEA Inferred from Electronic Annotation more info
involved_in regulation of bicellular tight junction assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cellular respiration	ISO Inferred from Sequence Orthology more info
involved_in regulation of osteoblast differentiation	IEA Inferred from Electronic Annotation more info
involved_in regulation of osteoblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in regulation of proteasomal protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of proteasomal protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of protein processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of synaptic transmission, glutamatergic	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in sperm capacitation	IDA Inferred from Direct Assay more info	PubMed
involved_in spontaneous exocytosis of neurotransmitter	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in acrosomal vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in axoneme	IEA Inferred from Electronic Annotation more info
colocalizes_with axoneme	ISO Inferred from Sequence Orthology more info
part_of cAMP-dependent protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cAMP-dependent protein kinase complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of cAMP-dependent protein kinase complex	ISO Inferred from Sequence Orthology more info
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in ciliary base	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in neuromuscular junction	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
part_of nucleotide-activated protein kinase complex	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane raft	IEA Inferred from Electronic Annotation more info
located_in plasma membrane raft	ISO Inferred from Sequence Orthology more info
located_in postsynapse	IEA Inferred from Electronic Annotation more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info
is_active_in presynapse	ISO Inferred from Sequence Orthology more info
located_in presynapse	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
located_in sperm flagellum	IDA Inferred from Direct Assay more info	PubMed
located_in sperm midpiece	ISO Inferred from Sequence Orthology more info
located_in sperm midpiece	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: cAMP-dependent protein kinase catalytic subunit alpha

Names: PKA C-alpha; sperm cAMP-dependent protein kinase catalytic subunit

NP_001264827.1

EC 2.7.11.11

NP_032880.1

EC 2.7.11.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001277898.1 → NP_001264827.1 cAMP-dependent protein kinase catalytic subunit alpha isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2, also known as Cs and Calpha2) contains an alternate 5'-most exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct and shorter N-terminus, compared to isoform 1. This variant is testis-specific and the encoded isoform does not contain the myristylation signal found in isoform 1 (PMID: 10841548 and PMID: 10982398). There are no publicly available full-length transcripts for this variant, but it is supported by publication data, including PMID: 10982398.

Source sequence(s)

AF239743, BQ031203, CB248596, CD807077

Consensus CDS

CCDS85574.1

UniProtKB/Swiss-Prot

P05132

Related

ENSMUSP00000147256.2, ENSMUST00000211558.2

Conserved Domains (1) summary

cd14209
Location:34 → 323

STKc_PKA; Catalytic subunit of the Serine/Threonine Kinase, cAMP-dependent protein kinase
NM_008854.5 → NP_032880.1 cAMP-dependent protein kinase catalytic subunit alpha isoform 1

See identical proteins and their annotated locations for NP_032880.1

Status: REVIEWED

Description

Transcript Variant: This variant (1, also known as Calpha1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC156028, BQ031203, BU059097, CB248596, CD807077

Consensus CDS

CCDS22463.1

UniProtKB/Swiss-Prot

P05132, Q9JID0

Related

ENSMUSP00000005606.7, ENSMUST00000005606.8

Conserved Domains (1) summary

cd14209
Location:42 → 331

STKc_PKA; Catalytic subunit of the Serine/Threonine Kinase, cAMP-dependent protein kinase