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    MPST mercaptopyruvate sulfurtransferase [ Homo sapiens (human) ]

    Gene ID: 4357, updated on 28-Oct-2024

    Summary

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    Official Symbol
    MPSTprovided by HGNC
    Official Full Name
    mercaptopyruvate sulfurtransferaseprovided by HGNC
    Primary source
    HGNC:HGNC:7223
    See related
    Ensembl:ENSG00000128309 MIM:602496; AllianceGenome:HGNC:7223
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    MST; TST2; TUM1
    Summary
    This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in duodenum (RPKM 33.5), liver (RPKM 30.3) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See MPST in Genome Data Viewer
    Location:
    22q12.3
    Exon count:
    5
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 22 NC_000022.11 (37019742..37029815)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 22 NC_060946.1 (37479341..37489414)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 22 NC_000022.10 (37415783..37425856)

    Chromosome 22 - NC_000022.11Genomic Context describing neighboring genes Neighboring gene uncharacterized protein FLJ39582-like Neighboring gene CRISPRi-validated cis-regulatory element chr22.1656 Neighboring gene CRISPRi-validated cis-regulatory element chr22.1657 Neighboring gene ciliary microtubule inner protein 4 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:37408066-37408566 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:37408567-37409067 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:37414031-37415006 Neighboring gene H3K27ac hESC enhancer GRCh37_chr22:37415035-37415536 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13676 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13675 Neighboring gene thiosulfate sulfurtransferase Neighboring gene uncharacterized LOC112268295 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:37428845-37429362 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:37429455-37430120 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:37439765-37440678 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13677 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:37447950-37448667 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:37448668-37449384 Neighboring gene RN7SK pseudogene 214 Neighboring gene potassium channel tetramerization domain containing 17

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. 3-Mercaptopyruvate sulfur transferase is a protein persulfidase. Pedre B, et al. Nat Chem Biol, 2023 Apr. PMID 36732619, Free PMC Article
    2. Cloning and sequence analysis of the human liver rhodanese: comparison with the bovine and chicken enzymes. Pallini R, et al. Biochem Biophys Res Commun, 1991 Oct 31. PMID 1953758
    3. Evidence for a functional genetic polymorphism of the human mercaptopyruvate sulfurtransferase (MPST), a cyanide detoxification enzyme. Billaut-Laden I, et al. Toxicol Lett, 2006 Aug 20. PMID 16545926
    4. Structure and kinetic analysis of H2S production by human mercaptopyruvate sulfurtransferase. Yadav PK, et al. J Biol Chem, 2013 Jul 5. PMID 23698001, Free PMC Article
    5. Characterization and interaction studies of two isoforms of the dual localized 3-mercaptopyruvate sulfurtransferase TUM1 from humans. Fräsdorf B, et al. J Biol Chem, 2014 Dec 12. PMID 25336638, Free PMC Article

    See all (45) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Role of the 3-mercaptopyruvate sulfurtransferase in colon/colorectal cancers.
      Title: Role of the 3-mercaptopyruvate sulfurtransferase in colon/colorectal cancers.
    2. 3-Mercaptopyruvate sulfur transferase is a protein persulfidase.
      Title: 3-Mercaptopyruvate sulfur transferase is a protein persulfidase.
    3. MPST deficiency promotes intestinal epithelial cell apoptosis and aggravates inflammatory bowel disease via AKT.
      Title: MPST deficiency promotes intestinal epithelial cell apoptosis and aggravates inflammatory bowel disease via AKT.
    4. 3-Mercaptopyruvate sulfurtransferase represses tumour progression and predicts prognosis in hepatocellular carcinoma.
      Title: 3-Mercaptopyruvate sulfurtransferase represses tumour progression and predicts prognosis in hepatocellular carcinoma.
    5. 3-Mercaptopyruvate sulfurtransferase supports endothelial cell angiogenesis and bioenergetics.
      Title: 3-Mercaptopyruvate sulfurtransferase supports endothelial cell angiogenesis and bioenergetics.
    6. Thioredoxin regulates human mercaptopyruvate sulfurtransferase at physiologically-relevant concentrations.
      Title: Thioredoxin regulates human mercaptopyruvate sulfurtransferase at physiologically-relevant concentrations.
    7. MPST inactivation or silencing enhances the plasticity of dermal fibroblasts in a TET1 and glycolysis dependent manner and promotes adipogenic transdifferentiation.
      Title: 3-Mercaptopyruvate sulfurtransferase disruption in dermal fibroblasts facilitates adipogenic trans-differentiation.
    8. 3-Mercaptopyruvate sulphurtransferase and not cystathionine gamma-lyase is the primary regulator of coronary artery hydrogen sulfide production and function.
      Title: MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery.
    9. In this review, we discuss the roles of non-canonical Hippo/Mst signaling pathways in lymphocyte development and functions. [review]
      Title: The non-canonical Hippo/Mst pathway in lymphocyte development and functions.
    10. distinct roles of each TUM1 isoform in the sulfur transfer processes in the cell
      Title: Characterization and interaction studies of two isoforms of the dual localized 3-mercaptopyruvate sulfurtransferase TUM1 from humans.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC24539

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables 3-mercaptopyruvate sulfurtransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3-mercaptopyruvate sulfurtransferase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables 3-mercaptopyruvate sulfurtransferase activity TAS
    Traceable Author Statement
    more info
    		  
    enables identical protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables thiosulfate sulfurtransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in cyanate catabolic process TAS
    Traceable Author Statement
    more info
    		  
    involved_in hydrogen sulfide biosynthetic process ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in kidney development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in liver development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to toxic substance TAS
    Traceable Author Statement
    more info
    		  
    involved_in spinal cord development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in sulfur amino acid catabolic process TAS
    Traceable Author Statement
    more info
    		  
    involved_in transsulfuration IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in extracellular exosome HDA PubMed 
    located_in mitochondrial matrix TAS
    Traceable Author Statement
    more info
    		  
    located_in mitochondrion HTP PubMed 
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in neuron projection ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in synapse IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    3-mercaptopyruvate sulfurtransferase
    Names
    human liver rhodanese
    tRNA thiouridin modification protein 1
    testicular tissue protein Li 200
    NP_001013454.1
    NP_001123989.1
    NP_001356833.1
    NP_001356834.1
    NP_066949.2
    XP_047297332.1
    XP_047297333.1
    XP_054181617.1
    XP_054181618.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001013436.4 → NP_001013454.1  3-mercaptopyruvate sulfurtransferase isoform 2

      See identical proteins and their annotated locations for NP_001013454.1

      Status: REVIEWED

      Source sequence(s)
      Z73420
      Consensus CDS
      CCDS13939.1
      UniProtKB/Swiss-Prot
      A8MZ34, B3KP52, J3KPV7, O75750, P25325, Q6FHN9
      UniProtKB/TrEMBL
      A0A140VJX3
      Related
      ENSP00000384812.3, ENST00000401419.7
      Conserved Domains (1) summary
      PLN02723
      Location:9 → 279
      PLN02723; 3-mercaptopyruvate sulfurtransferase

    2. NM_001130517.4 → NP_001123989.1  3-mercaptopyruvate sulfurtransferase isoform 2

      See identical proteins and their annotated locations for NP_001123989.1

      Status: REVIEWED

      Source sequence(s)
      Z73420
      Consensus CDS
      CCDS13939.1
      UniProtKB/Swiss-Prot
      A8MZ34, B3KP52, J3KPV7, O75750, P25325, Q6FHN9
      UniProtKB/TrEMBL
      A0A140VJX3
      Related
      ENSP00000383950.3, ENST00000404802.7
      Conserved Domains (1) summary
      PLN02723
      Location:9 → 279
      PLN02723; 3-mercaptopyruvate sulfurtransferase

    3. NM_001369904.2 → NP_001356833.1  3-mercaptopyruvate sulfurtransferase isoform 2

      Status: REVIEWED

      Source sequence(s)
      Z73420
      Consensus CDS
      CCDS13939.1
      UniProtKB/Swiss-Prot
      A8MZ34, B3KP52, J3KPV7, O75750, P25325, Q6FHN9
      UniProtKB/TrEMBL
      A0A140VJX3
      Related
      ENSP00000342333.3, ENST00000341116.7
      Conserved Domains (1) summary
      PLN02723
      Location:9 → 279
      PLN02723; 3-mercaptopyruvate sulfurtransferase

    4. NM_001369905.2 → NP_001356834.1  3-mercaptopyruvate sulfurtransferase isoform 3

      Status: REVIEWED

      Source sequence(s)
      Z73420
      UniProtKB/TrEMBL
      B1AH49
      Conserved Domains (1) summary
      COG2897
      Location:16 → 219
      SseA; 3-mercaptopyruvate sulfurtransferase SseA, contains two rhodanese domains [Inorganic ion transport and metabolism]

    5. NM_021126.8 → NP_066949.2  3-mercaptopyruvate sulfurtransferase isoform 1

      See identical proteins and their annotated locations for NP_066949.2

      Status: REVIEWED

      Source sequence(s)
      BC016737, Z73420
      Consensus CDS
      CCDS46703.1
      UniProtKB/Swiss-Prot
      P25325
      Related
      ENSP00000411719.3, ENST00000429360.6
      Conserved Domains (3) summary
      PLN02723
      Location:6 → 299
      PLN02723; 3-mercaptopyruvate sulfurtransferase
      cd01448
      Location:30 → 159
      TST_Repeat_1; Thiosulfate sulfurtransferase (TST), N-terminal, inactive domain. TST contains 2 copies of the Rhodanese Homology Domain; this is the 1st repeat, which does not contain the catalytically active Cys residue. The role of the 1st repeat is uncertain, but it ...
      cd01449
      Location:184 → 299
      TST_Repeat_2; Thiosulfate sulfurtransferase (TST), C-terminal, catalytic domain. TST contains 2 copies of the Rhodanese Homology Domain; this is the second repeat. Only the second repeat contains the catalytically active Cys residue.

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000022.11 Reference GRCh38.p14 Primary Assembly

      Range
      37019742..37029815
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047441376.1 → XP_047297332.1  3-mercaptopyruvate sulfurtransferase isoform X1

    2. XM_047441377.1 → XP_047297333.1  3-mercaptopyruvate sulfurtransferase isoform X2

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060946.1 Alternate T2T-CHM13v2.0

      Range
      37479341..37489414
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054325642.1 → XP_054181617.1  3-mercaptopyruvate sulfurtransferase isoform X1

    2. XM_054325643.1 → XP_054181618.1  3-mercaptopyruvate sulfurtransferase isoform X2

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001013440.1: Suppressed sequence

      Description
      NM_001013440.1: This RefSeq was permanently suppressed because it was annotated to initiate translation from a downstream AUG when an upstream AUG exists, initiation from which will render this RefSeq susceptible to nonsense-mediated mRNA decay (NMD).

    2. NR_024038.1: Suppressed sequence

      Description
      NR_024038.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript.
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P25325.3 GenPept UniProtKB/Swiss-Prot:P25325

    Gene LinkOut

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