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    BRD4 bromodomain containing 4 [ Homo sapiens (human) ]

    Gene ID: 23476, updated on 28-Oct-2024

    Summary

    Official Symbol
    BRD4provided by HGNC
    Official Full Name
    bromodomain containing 4provided by HGNC
    Primary source
    HGNC:HGNC:13575
    See related
    Ensembl:ENSG00000141867 MIM:608749; AllianceGenome:HGNC:13575
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    CAP; MCAP; CDLS6; HUNK1; HUNKI; FSHRG4
    Summary
    The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in placenta (RPKM 10.8), testis (RPKM 9.2) and 25 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See BRD4 in Genome Data Viewer
    Location:
    19p13.12
    Exon count:
    26
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (15235519..15332539, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (15360431..15457479, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (15346330..15443350, complement)

    Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene RNA, U6 small nuclear 782, pseudogene Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:15281145-15281735 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10267 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:15291996-15292515 Neighboring gene notch receptor 3 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:15292516-15293034 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15296283-15296783 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15305376-15305876 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15305877-15306377 Neighboring gene microRNA 6795 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:15310876-15311102 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15311689-15312190 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15312191-15312690 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:15333321-15334017 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10268 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15343009-15343510 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10269 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15361355-15362226 Neighboring gene epoxide hydrolase 3 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:15373316-15373494 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:15374509-15375008 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15376180-15376680 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:15385287-15385786 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14182 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14183 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:15409795-15410322 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:15410749-15411456 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10270 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10271 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10272 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10273 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10274 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:15443616-15444232 Neighboring gene uncharacterized LOC124904643 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10276 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14184 Neighboring gene H3K27ac hESC enhancers GRCh37_chr19:15489739-15490654 and GRCh37_chr19:15490655-15491569 Neighboring gene A-kinase anchoring protein 8 Neighboring gene A-kinase anchoring protein 8 like Neighboring gene NANOG hESC enhancer GRCh37_chr19:15523607-15524136 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15524849-15525350 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:15525351-15525850

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    HIV-1 replication is inhibited by BRD4; siRNA-mediated knockdown allows for Tat-induced latency reversal PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat The RxL or KxL motif and the polybasic cluster of HIV-1 Tat and BRD4 exhibit similar importance for P-TEFb activation PubMed
    tat The C-terminal 34 residues (1329-1362) of BRD4 are crucial for interaction with P-TEFb. Overexpression of the BRD4 P-TEFb-interacting domain disrupts the interaction between HIV-1 Tat and P-TEFb PubMed
    tat CDK9-CycT1-AFF1 is stimulated by HIV-1 Tat and transferred as a single complex unit to BRD4 upon stress-induced disruption of AFF1-containing 7SK snRNP (HEXIM1, MEPCE, LARP7, 7SK RNA, CDK9, CycT1, and AFF1) PubMed
    tat Phosphorylation of CDK9 at position Ser175 regulates the competition between HIV-1 Tat and BRD4 for P-TEFb binding PubMed
    tat Brd4 inhibits HIV-1 Tat-human super elongation complex (components AFF4, ELL2, CycT1, and CDK9) by competing with HIV-1 Tat for binding to P-TEFb on HIV-1 promoter PubMed
    tat JQ1, a small molecule inhibitor of Brd4, directly dissociates Brd4 from HIV-1 promoter and promotes subsequently HIV-1 Tat binding to HIV-1 promoter, resulting in increased phosphorylation of RNA polymerase II and viral transcription PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables P-TEFb complex binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables RNA polymerase II C-terminal domain binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables RNA polymerase II CTD heptapeptide repeat kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    enables chromatin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables chromatin binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables enzyme binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables histone binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables histone reader activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables lysine-acetylated histone binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables p53 binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein serine/threonine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables transcription cis-regulatory region binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables transcription coactivator activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    enables transcription coregulator activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in DNA damage response IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in DNA damage response IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in chromatin organization IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in chromatin remodeling IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in chromatin remodeling IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation by host of viral transcription IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation of DNA damage checkpoint IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of DNA-templated transcription IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    acts_upstream_of_or_within positive regulation of G2/M transition of mitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of T-helper 17 cell lineage commitment ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of canonical NF-kappaB signal transduction IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of transcription elongation by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    PubMed 
    acts_upstream_of_or_within positive regulation of transcription elongation by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulation of inflammatory response IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    acts_upstream_of_or_within regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in chromatin IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in chromosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in condensed nuclear chromosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    bromodomain-containing protein 4
    Names
    chromosome-associated protein
    female sterile homeotic related gene 4
    mitotic chromosome-associated protein
    NP_001317313.1
    NP_001366220.1
    NP_001366221.1
    NP_055114.1
    NP_490597.1
    XP_047294496.1
    XP_047294497.1
    XP_047294498.1
    XP_047294499.1
    XP_047294500.1
    XP_054176374.1
    XP_054176375.1
    XP_054176376.1
    XP_054176377.1
    XP_054176378.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001330384.2NP_001317313.1  bromodomain-containing protein 4 isoform 3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (short) differs in the 3' UTR and coding region compared to variant long. The resulting isoform (short) is shorter and has a distinct C-terminus compared to isoform long.
      Source sequence(s)
      AC004798, AC005776, BC035266
      Consensus CDS
      CCDS82307.1
      Related
      ENSP00000353112.4, ENST00000360016.9
      Conserved Domains (3) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
    2. NM_001379291.1NP_001366220.1  bromodomain-containing protein 4 isoform long

      Status: REVIEWED

      Source sequence(s)
      AC004257, AC004798, AC005776
      Consensus CDS
      CCDS12328.1
      UniProtKB/Swiss-Prot
      O60433, O60885, Q4G0X8, Q86YS8, Q96PD3
      Related
      ENSP00000506350.1, ENST00000679869.1
      Conserved Domains (5) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      PRK03427
      Location:809922
      PRK03427; cell division protein ZipA; Provisional
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
      pfam17105
      Location:13191362
      BRD4_CDT; C-terminal domain of bromodomain protein 4
    3. NM_001379292.1NP_001366221.1  bromodomain-containing protein 4 isoform short

      Status: REVIEWED

      Source sequence(s)
      AC004798, AC005776
      Consensus CDS
      CCDS46004.1
      Conserved Domains (3) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
    4. NM_014299.3NP_055114.1  bromodomain-containing protein 4 isoform short

      See identical proteins and their annotated locations for NP_055114.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (short) differs in the 3' UTR and coding region compared to variant long. The resulting isoform (short) is shorter and has a distinct C-terminus compared to isoform long.
      Source sequence(s)
      AC004798, BC035266, Y12059
      Consensus CDS
      CCDS46004.1
      UniProtKB/Swiss-Prot
      O60885
      Related
      ENSP00000360901.3, ENST00000371835.8
      Conserved Domains (3) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
    5. NM_058243.3NP_490597.1  bromodomain-containing protein 4 isoform long

      See identical proteins and their annotated locations for NP_490597.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (long) encodes the full length isoform.
      Source sequence(s)
      AC004257, AC004798, AF386649, BC035266
      Consensus CDS
      CCDS12328.1
      UniProtKB/Swiss-Prot
      O60433, O60885, Q4G0X8, Q86YS8, Q96PD3
      Related
      ENSP00000263377.1, ENST00000263377.6
      Conserved Domains (5) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      PRK03427
      Location:809922
      PRK03427; cell division protein ZipA; Provisional
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
      pfam17105
      Location:13191362
      BRD4_CDT; C-terminal domain of bromodomain protein 4

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

      Range
      15235519..15332539 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047438540.1XP_047294496.1  bromodomain-containing protein 4 isoform X1

      UniProtKB/Swiss-Prot
      O60433, O60885, Q4G0X8, Q86YS8, Q96PD3
    2. XM_047438541.1XP_047294497.1  bromodomain-containing protein 4 isoform X2

    3. XM_047438543.1XP_047294499.1  bromodomain-containing protein 4 isoform X4

    4. XM_047438544.1XP_047294500.1  bromodomain-containing protein 4 isoform X4

    5. XM_047438542.1XP_047294498.1  bromodomain-containing protein 4 isoform X3

    RNA

    1. XR_007066712.1 RNA Sequence

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060943.1 Alternate T2T-CHM13v2.0

      Range
      15360431..15457479 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054320399.1XP_054176374.1  bromodomain-containing protein 4 isoform X1

      UniProtKB/Swiss-Prot
      O60433, O60885, Q4G0X8, Q86YS8, Q96PD3
    2. XM_054320400.1XP_054176375.1  bromodomain-containing protein 4 isoform X2

    3. XM_054320403.1XP_054176378.1  bromodomain-containing protein 4 isoform X4

    4. XM_054320402.1XP_054176377.1  bromodomain-containing protein 4 isoform X4

    5. XM_054320401.1XP_054176376.1  bromodomain-containing protein 4 isoform X3

    RNA

    1. XR_008485130.1 RNA Sequence

    2. XR_008485129.1 RNA Sequence