ROCK1 Rho associated coiled-coil containing protein kinase 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: ROCK1provided by HGNC
Official Full Name: Rho associated coiled-coil containing protein kinase 1provided by HGNC
Primary source: HGNC:HGNC:10251
See related: Ensembl:ENSG00000067900 MIM:601702; AllianceGenome:HGNC:10251
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: ROCK-I; P160ROCK
Summary: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]
Expression: Ubiquitous expression in bone marrow (RPKM 22.1), fat (RPKM 16.6) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 18q11.1

Exon count:: 33

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	18	NC_000018.10 (20946906..21111813, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	18	NC_060942.1 (21131976..21296884, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	18	NC_000018.9 (18526867..18691774, complement)

Chromosome 18 - NC_000018.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inhibition of the FOXO1-ROCK1 axis mitigates cardiomyocyte injury under chronic hypoxia in Tetralogy of Fallot by maintaining mitochondrial quality control.
Title: Inhibition of the FOXO1-ROCK1 axis mitigates cardiomyocyte injury under chronic hypoxia in Tetralogy of Fallot by maintaining mitochondrial quality control.
TRIM40 interacts with ROCK1 directly and inhibits colorectal cancer cell proliferation through the c-Myc/p21 axis.
Title: TRIM40 interacts with ROCK1 directly and inhibits colorectal cancer cell proliferation through the c-Myc/p21 axis.
MiR-599 regulates LPS-mediated apoptosis and inflammatory responses through the JAK2/STAT3 signalling pathway via targeting ROCK1 in human umbilical vein endothelial cells.
Title: MiR-599 regulates LPS-mediated apoptosis and inflammatory responses through the JAK2/STAT3 signalling pathway via targeting ROCK1 in human umbilical vein endothelial cells.
ROCK1/2 signaling contributes to corticosteroid-refractory acute graft-versus-host disease.
Title: ROCK1/2 signaling contributes to corticosteroid-refractory acute graft-versus-host disease.
LINC00645 inhibits renal cell carcinoma progression by interacting with HNRNPA2B1 to regulate the ROCK1 mRNA stability.
Title: LINC00645 inhibits renal cell carcinoma progression by interacting with HNRNPA2B1 to regulate the ROCK1 mRNA stability.
LAMB3 Promotes Myofibrogenesis and Cytoskeletal Reorganization in Endometrial Stromal Cells via the RhoA/ROCK1/MYL9 Pathway.
Title: LAMB3 Promotes Myofibrogenesis and Cytoskeletal Reorganization in Endometrial Stromal Cells via the RhoA/ROCK1/MYL9 Pathway.
Paxillin participates in the sphingosylphosphorylcholine-induced abnormal contraction of vascular smooth muscle by regulating Rho-kinase activation.
Title: Paxillin participates in the sphingosylphosphorylcholine-induced abnormal contraction of vascular smooth muscle by regulating Rho-kinase activation.
Identification of ROCK1 as a novel biomarker for postmenopausal osteoporosis and pan-cancer analysis.
Title: Identification of ROCK1 as a novel biomarker for postmenopausal osteoporosis and pan-cancer analysis.
lncRNA NEAT1 mediates LPS-induced pyroptosis of BEAS-2B cells via targeting miR-26a-5p/ROCK1 axis.
Title: lncRNA NEAT1 mediates LPS-induced pyroptosis of BEAS-2B cells via targeting miR-26a-5p/ROCK1 axis.
Negative pressure induces dedifferentiation of hepatocytes via RhoA/ROCK pathway.
Title: Negative pressure induces dedifferentiation of hepatocytes via RhoA/ROCK pathway.

Submit: New GeneRIF Correction See all GeneRIFs (402)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
HIV-1 infection (VSV-G pseudotyped) of CEMT4 T cells downregulates plasma membrane expression of ROCK1	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	Filamin-A-dependent activation of the RhoA-ROCK-LIMK-cofilin pathway is a major event in HIV-1 gp120-induced receptor clustering	PubMed
Tat	tat	HIV-1 Tat downregulates OCLN (occludin) and LRP1 and upregulates AGER (RAGE), and in a RhoA/ROCK1 dependent manner as shown through hydroxyfasudil inhibition in hCMEC/D3 cells	PubMed
	tat	HIV-1 Tat-mediated downregulation of occludin expression requires activities of the RhoA and p160-Rho-associated coiled kinase (ROCK) proteins in primary human brain micro vascular endothelial cells	PubMed
matrix	gag	HIV-1 MA-induced upregulation of collagen-I and alpha-SMA expression is dependent on CXCR2 via the Rho/ROCK-1 pathway	PubMed
	gag	HIV-1 p17-mediated activation of the Rho/ROCK-signaling pathway is required for p17-mediated monocyte adhesion and migration	PubMed
	gag	HIV-1 p17-induced activation of PTEN is regulated by RhoA-associated kinase (ROCK)	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH78771 (e-PCR)
- UniSTS:86028

RH65142 (e-PCR)
- UniSTS:88128

RH122410 (e-PCR)
- UniSTS:135280

RH78137 (e-PCR)
- UniSTS:77191

ROCK1 (e-PCR)
- UniSTS:504235

RH65143 (e-PCR)
- UniSTS:37095

D18S374E (e-PCR)
- UniSTS:473078

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables Rho-dependent protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	TAS Traceable Author Statement more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	NAS Non-traceable Author Statement more info	PubMed
enables protein serine/threonine kinase activity	TAS Traceable Author Statement more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables tau protein binding	NAS Non-traceable Author Statement more info	PubMed
enables tau-protein kinase activity	NAS Non-traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in Rho protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in actin cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in actomyosin structure organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in aortic valve morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in apical constriction	IEA Inferred from Electronic Annotation more info
involved_in bleb assembly	IEA Inferred from Electronic Annotation more info
involved_in blood vessel diameter maintenance	ISS Inferred from Sequence or Structural Similarity more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in cortical actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in embryonic morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in epithelial to mesenchymal transition	ISS Inferred from Sequence or Structural Similarity more info
involved_in leukocyte cell-cell adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in leukocyte migration	IDA Inferred from Direct Assay more info	PubMed
involved_in leukocyte tethering or rolling	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA destabilization	ISS Inferred from Sequence or Structural Similarity more info
involved_in membrane to membrane docking	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in motor neuron apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in myoblast migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of amyloid precursor protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of amyloid-beta formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of bicellular tight junction assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of biomineral tissue development	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of membrane protein ectodomain proteolysis	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of motor neuron apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein binding	IDA Inferred from Direct Assay more info	PubMed
involved_in neuron projection arborization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron projection development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in podocyte cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of amyloid-beta clearance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of autophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cardiac muscle hypertrophy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of connective tissue replacement	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of dephosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of focal adhesion assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of phosphatase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to plasma membrane	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of actin cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of actin cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of actin cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
involved_in regulation of angiotensin-activated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell adhesion	TAS Traceable Author Statement more info	PubMed
involved_in regulation of cell junction assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell migration	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell motility	TAS Traceable Author Statement more info	PubMed
involved_in regulation of establishment of cell polarity	TAS Traceable Author Statement more info	PubMed
involved_in regulation of establishment of endothelial barrier	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of establishment of endothelial barrier	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of focal adhesion assembly	TAS Traceable Author Statement more info	PubMed
involved_in regulation of keratinocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of microtubule cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of stress fiber assembly	TAS Traceable Author Statement more info	PubMed
involved_in regulation of synapse maturation	IEA Inferred from Electronic Annotation more info
involved_in regulation of synaptic vesicle endocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to angiotensin	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to transforming growth factor beta	ISS Inferred from Sequence or Structural Similarity more info
involved_in signal transduction	TAS Traceable Author Statement more info	PubMed
involved_in smooth muscle contraction	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in Schaffer collateral - CA1 synapse	IEA Inferred from Electronic Annotation more info
located_in bleb	IEA Inferred from Electronic Annotation more info
located_in centriole	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasmic stress granule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic stress granule	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	TAS Traceable Author Statement more info
located_in extracellular region	TAS Traceable Author Statement more info
located_in lamellipodium	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in ruffle	ISS Inferred from Sequence or Structural Similarity more info
located_in secretory granule lumen	TAS Traceable Author Statement more info

General protein information

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Preferred Names: rho-associated protein kinase 1

Names: p160 ROCK-1; renal carcinoma antigen NY-REN-35

NP_005397.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_042178.2 RefSeqGene

Range

5039..169946

Download

GenBank, FASTA, Sequence Viewer (Graphics), LRG_1005

mRNA and Protein(s)

NM_005406.3 → NP_005397.1 rho-associated protein kinase 1

See identical proteins and their annotated locations for NP_005397.1

Status: REVIEWED

Source sequence(s)

AC036178, AK057038, AU121527, U43195

Consensus CDS

CCDS11870.2

UniProtKB/Swiss-Prot

B0YJ91, Q13464, Q2KHM4, Q59GZ4

UniProtKB/TrEMBL

D9ZGF8

Related

ENSP00000382697.1, ENST00000399799.3

Conserved Domains (7) summary

cd11639
Location:489 → 554

HR1_ROCK1; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 1

smart00220
Location:76 → 338

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG0419
Location:422 → 1027

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd00029
Location:1229 → 1275

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1119 → 1225

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05622
Location:2 → 406

STKc_ROCK1; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase 1

pfam08912
Location:949 → 1014

Rho_Binding; Rho Binding