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    VRP1 Vrp1p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851051, updated on 18-Sep-2024

    Summary

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    Official Symbol
    VRP1
    Official Full Name
    Vrp1p
    Primary source
    SGD:S000004329
    Locus tag
    YLR337C
    See related
    AllianceGenome:SGD:S000004329; FungiDB:YLR337C; VEuPathDB:YLR337C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    END5; MDP2; YLR337W
    Summary
    Enables actin binding activity. Involved in several processes, including actin cortical patch localization; bipolar cellular bud site selection; and positive regulation of Arp2/3 complex-mediated actin nucleation. Located in actin cortical patch and cellular bud neck. Part of incipient cellular bud site. Orthologous to several human genes including WIPF1 (WAS/WASL interacting protein family member 1). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See VRP1 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (802653..805106, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene nucleoporin NUP2 Neighboring gene Sgd1p Neighboring gene ribosomal protein P0 Neighboring gene Spo77p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A proline-rich protein, verprolin, involved in cytoskeletal organization and cellular growth in the yeast Saccharomyces cerevisiae. Donnelly SF, et al. Mol Microbiol, 1993 Nov. PMID 7968536
    2. Actin binding and proline rich motifs of CR16 play redundant role in growth of vrp1Delta cells. Meng L, et al. Biochem Biophys Res Commun, 2007 May 25. PMID 17418095
    3. Screening for a gene deletion mutant whose temperature sensitivity is suppressed by FK506 in budding yeast and its application for a positive screening for drugs inhibiting calcineurin. Kume K, et al. Biosci Biotechnol Biochem, 2015. PMID 25614218
    4. Functions of Vrp1p in cytokinesis and actin patches are distinct and neither requires a WH2/V domain. Thanabalu T, et al. EMBO J, 2001 Dec 17. PMID 11742975, Free PMC Article
    5. The human WASP-interacting protein, WIP, activates the cell polarity pathway in yeast. Vaduva G, et al. J Biol Chem, 1999 Jun 11. PMID 10358064

    See all (137) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. These studies provide evidence that WASP (Las17) and WIP (Vrp1) play central roles in establishment of a robust multivalent Src homology 3 domain-proline-rich motif network in vivo, giving Actin-related protein 2/3 complex assembly onset at endocytic sites a switch-like behavior.
      Title: Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo.
    2. Vrp1 protin is responsible for temperature sensitivity and plays a role in calcineurin activation and inhibition.
      Title: Screening for a gene deletion mutant whose temperature sensitivity is suppressed by FK506 in budding yeast and its application for a positive screening for drugs inhibiting calcineurin.
    3. Results identify an N-terminal fragment of Las17p able to interact with Vrp1p but not Arp2/3, and suggest that LAs17 interaction with Vrp1 is more important than interaction with Arp2/3 for polarized actin assembly and endocytosis in yeast.
      Title: Las17p-Vrp1p but not Las17p-Arp2/3 interaction is important for actin patch polarization in yeast.
    4. Findings highlight the role of Las17p binding for Vrp1p membrane association, suggest general membrane association may be more important than specific targeting to the cortical actin cytoskeleton for Vrp1p. function in endocytosis and cell growth.
      Title: Verprolin function in endocytosis and actin organization. Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables actin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables actin binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in actin cortical patch localization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in bipolar cellular bud site selection IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in endocytosis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of Arp2/3 complex-mediated actin nucleation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of cytokinesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in actin cortical patch IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cellular bud neck IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in incipient cellular bud site IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mating projection tip HDA PubMed 

    General protein information

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    Preferred Names
    Vrp1p
    NP_013441.1
    • Verprolin, proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      802653..805106 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182226.1 → NP_013441.1  TPA: Vrp1p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013441.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VYX7, P37370, Q06133
      UniProtKB/TrEMBL
      E9PA85, N1NZN0
      Conserved Domains (2) summary
      PRK14951
      Location:109 → 240
      PRK14951; DNA polymerase III subunits gamma and tau; Provisional
      cd22064
      Location:27 → 55
      WH2_WAS_WASL; Wiskott Aldrich syndrome homology region 2 (WH2 motif) in WAS/WASL-interacting protein (WIP)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P37370.2

    Gene LinkOut

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