NEJ1 Nej1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NEJ1
Official Full Name: Nej1p
Primary source: SGD:S000004255
Locus tag: YLR265C
See related: AllianceGenome:SGD:S000004255; FungiDB:YLR265C; VEuPathDB:YLR265C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: LIF2
Summary: Enables DNA end binding activity. Involved in DNA metabolic process and positive regulation of DNA ligase activity. Located in nucleus. Part of DNA ligase IV complex. Is active in site of double-strand break. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See NEJ1 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (674427..675455, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nej1 interacts with Sae2 at DNA double-stranded breaks to inhibit DNA resection.
Title: Nej1 interacts with Sae2 at DNA double-stranded breaks to inhibit DNA resection.
The conserved Tpk1 regulates non-homologous end joining double-strand break repair by phosphorylation of Nej1, a homolog of the human XLF.
Title: The conserved Tpk1 regulates non-homologous end joining double-strand break repair by phosphorylation of Nej1, a homolog of the human XLF.
Our results reveal novel roles for Nej1 in the recruitment of Pol4 and Rad27 to in vivo DNA double strand breaks and the coordination of the end processing and ligation reactions of non-homologous end joining.
Title: Role of the yeast DNA repair protein Nej1 in end processing during the repair of DNA double strand breaks by non-homologous end joining.
C-terminus of Nej1 is critical for nuclear localization and non-homologous end-joining
Title: The C-terminus of Nej1 is critical for nuclear localization and non-homologous end-joining.
Nej1 not only plays an important role in determining repair pathway choice by participating in the initial NHEJ complex formed at DSBs but also contributes to the reactivation of Dnl4-Lif1 after repair is complete
Title: Yeast Nej1 is a key participant in the initial end binding and final ligation steps of nonhomologous end joining.
Nej1 physically interacts with Srs2.
Title: Nej1 recruits the Srs2 helicase to DNA double-strand breaks and supports repair by a single-strand annealing-like mechanism.
Structural characterization reveals that Nej1p exists as a dimer, and that residues 1-244 are sufficient for dimer formation
Title: A critical role for the C-terminus of Nej1 protein in Lif1p association, DNA binding and non-homologous end-joining.
Nej1 interacts with Lif1 and supports NHEJ, similar to the distantly related mammalian Nej1 orthologue XLF
Title: Modes of interaction among yeast Nej1, Lif1 and Dnl4 proteins and comparison to human XLF, XRCC4 and Lig4.
Nej1p is phosphorylated in response to DNA damage in a manner that relies on the DNA damage checkpoint kinases Mec1p, Rad53p and Dun1p.
Title: The non-homologous end-joining protein Nej1p is a target of the DNA damage checkpoint.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA end binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA end binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA end binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via nonhomologous end joining	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via single-strand annealing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA ligase activity	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of DNA ligase IV complex	IBA Inferred from Biological aspect of Ancestor more info
part_of DNA ligase IV complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Nej1p

NP_013367.1

DNA end-binding protein required for nonhomologous end joining (NHEJ); subunit of the DNA ligase IV complex involved in initial end binding, intermolecular ligation, and DNA ligase reactivation after completion of repair; phosphorylation by Tpk1p is required for DNA damage induced NHEJ and for nuclear targeting of Nej1p and Lif1p; human homolog NHEJ1 is phosphorylated by human Tpk1p homolog PRKACB, regulating NHEJ; transcriptionally repressed by MAT heterozygosity