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    VPS45 Vps45p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852785, updated on 18-Sep-2024

    Summary

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    Official Symbol
    VPS45
    Official Full Name
    Vps45p
    Primary source
    SGD:S000003063
    Locus tag
    YGL095C
    See related
    AllianceGenome:SGD:S000003063; FungiDB:YGL095C; VEuPathDB:YGL095C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    STT10; VPL28
    Summary
    Enables SNARE binding activity and unfolded protein binding activity. Involved in several processes, including Golgi vesicle transport; positive regulation of SNARE complex assembly; and vacuole inheritance. Located in Golgi membrane and cytosol. Is extrinsic component of membrane. Part of SNARE complex. Human ortholog(s) of this gene implicated in severe congenital neutropenia 5. Orthologous to human VPS45 (vacuolar protein sorting 45 homolog). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See VPS45 in Genome Data Viewer
    Location:
    chromosome: VII
    Exon count:
    1
    Sequence:
    Chromosome: VII; NC_001139.9 (328874..330607, complement)

    Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene Tos8p Neighboring gene tRNA-Glu Neighboring gene poly(A)-specific ribonuclease Neighboring gene Spc105p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001139.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Traffic into the prevacuolar/endosomal compartment of Saccharomyces cerevisiae: a VPS45-dependent intracellular route and a VPS45-independent, endocytic route. Bryant NJ, et al. Eur J Cell Biol, 1998 May. PMID 9650782
    2. Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles. Cowles CR, et al. J Cell Sci, 1994 Dec. PMID 7706396
    3. The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. Furgason ML, et al. Proc Natl Acad Sci U S A, 2009 Aug 25. PMID 19667197, Free PMC Article
    4. An Arabidopsis VPS45p homolog implicated in protein transport to the vacuole. Bassham DC, et al. Plant Physiol, 1998 Jun. PMID 9625693, Free PMC Article
    5. Cellular levels of the syntaxin Tlg2p are regulated by a single mode of binding to Vps45p. Carpp LN, et al. Biochem Biophys Res Commun, 2007 Nov 23. PMID 17904527

    See all (94) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that the SM protein Vps45p, which regulates endosomal trafficking in yeast, binds the conserved N-terminal peptide of the syntaxin Tlg2p.
      Title: The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p.
    2. Disruption of this high-affinity binding between Vps45p and Tlg2p leads to downregulation of Tlg2p.
      Title: Cellular levels of the syntaxin Tlg2p are regulated by a single mode of binding to Vps45p.
    3. Vps45p binds its cognate SNARE proteins through distinct mechanisms at different stages in the SNARE assembly/disassembly cycle.
      Title: The Sec1p/Munc18 protein Vps45p binds its cognate SNARE proteins via two distinct modes.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables SNARE binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables unfolded protein binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables unfolded protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Golgi to endosome transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in Golgi to vacuole transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in Golgi vesicle fusion to target membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cytoplasm to vacuole targeting by the Cvt pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in intracellular protein transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of SNARE complex assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein targeting to vacuole IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in vacuolar acidification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in vacuole inheritance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in vacuole organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in vesicle-mediated transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in vesicle-mediated transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in Golgi membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in Golgi membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of SNARE complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endomembrane system IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in intracellular membrane-bounded organelle IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vacuolar membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vacuole IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Vps45p
    NP_011420.3
    • Protein of the Sec1p/Munc-18 family; essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment; mutation in human VPS45 is associated with congenital neutropenia and primary myelofibrosis of infancy

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001139.9 Reference assembly

      Range
      328874..330607 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180960.3 → NP_011420.3  TPA: Vps45p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_011420.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VU50, P38932
      UniProtKB/TrEMBL
      A6ZU85, B3LHG0, B5VIN1, N1P5F0
      Conserved Domains (1) summary
      COG5158
      Location:3 → 577
      SEC1; Proteins involved in synaptic transmission and general secretion, Sec1 family [Intracellular trafficking and secretion]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P38932.2

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