MET30 ubiquitin-binding SDF ubiquitin ligase complex subunit MET30 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MET30
Official Full Name: ubiquitin-binding SDF ubiquitin ligase complex subunit MET30
Primary source: SGD:S000001308
Locus tag: YIL046W
See related: AllianceGenome:SGD:S000001308; FungiDB:YIL046W; VEuPathDB:YIL046W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ZRG11
Summary: Enables ubiquitin binding activity. Involved in several processes, including G1/S transition of mitotic cell cycle; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; and response to cadmium ion. Located in nucleus. Part of nuclear SCF ubiquitin ligase complex. Human ortholog(s) of this gene implicated in developmental delay, hypotonia, and impaired language. Orthologous to human FBXW7 (F-box and WD repeat domain containing 7). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See MET30 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (268651..270573)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cdc48 cofactor Shp1 regulates signal-induced SCF(Met30) disassembly.
Title: Cdc48 cofactor Shp1 regulates signal-induced SCF(Met30) disassembly.
Study revealed that two essential Skp, Cullin, F-box (SCF) ubiquitin ligases defined by the F-box proteins Met30 and Cdc4 cooperatively regulate Cse4 (S. cerevisiae homolog of human centromere protein-A (CENP-A)) proteolysis under normal physiological conditions. Results suggest SCF-Met30/Cdc4-mediated proteolysis of Cse4 is one of the major mechanisms that prevents stable maintenance of Cse4 at non-centromeric regions.
Title: Skp, Cullin, F-box (SCF)-Met30 and SCF-Cdc4-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A for Chromosomal Stability in Budding Yeast.
This ubiquitylation and degradation pathway acts on Met30 that is dissociated from Skp1. Unexpectedly, this pathway required the cullin component Cdc53/Cul1 but was independent of the other central SCF component Skp1
Title: Skp1 Independent Function of Cdc53/Cul1 in F-box Protein Homeostasis.
In Saccharomyces cerevisiae, transcription of the MET regulon is controlled by ubiquitination of the transcription factor Met4, which is carried out by the SCF(Met30) E3 ubiquitin ligase.
Title: Multiple inputs control sulfur-containing amino acid synthesis in Saccharomyces cerevisiae.
The stability of these DNA-binding cofactors is regulated by SCF(Met30).
Title: A transcriptional activator is part of an SCF ubiquitin ligase to control degradation of its cofactors.
Met32 has a regulatory role as part of the Cbf1-Met4 complex as shown by the met30 cell cycle defect
Title: A dominant suppressor mutation of the met30 cell cycle defect suggests regulation of the Saccharomyces cerevisiae Met4-Cbf1 transcription complex by Met32.
the recruitment of Met4p to the SCF(Met30p) complex itself results in inactivation of Met4p, independently of its ubiquitination
Title: Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p.
The association of Met30 with Skp1 but not with its substrate Met4 was inhibited in cells treated with cadmium
Title: The yeast ubiquitin ligase SCFMet30 regulates heavy metal response.
data suggest that Met30 regulates several aspects of the cell cycle, including G(1)-specific transcription, initiation of DNA replication, and progression through M phase
Title: The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cysteine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in methionine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein polyubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to arsenic-containing substance	IDA Inferred from Direct Assay more info	PubMed
involved_in response to cadmium ion	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of nuclear SCF ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nuclear SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ubiquitin-binding SDF ubiquitin ligase complex subunit MET30

NP_012218.1

F-box protein containing five copies of the WD40 motif; controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus; dissociation of Met30p from SCF complex in response to cadmium stress is regulated by Cdc48p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

268651..270573

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179396.1 → NP_012218.1 TPA: ubiquitin-binding SDF ubiquitin ligase complex subunit MET30 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012218.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVN6, P39014

UniProtKB/TrEMBL

A6ZVM3, B5VKQ1, G2WGA0, N1P0L8

Conserved Domains (3) summary

cd00200
Location:295 → 590

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:305 → 340

7WD40; WD40 repeat [structural motif]

pfam12937
Location:184 → 228

F-box-like