ADE3 trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ADE3
Gene description: trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3
Primary source: FungiDB:YGR204W
Locus tag: YGR204W
See related: SGD:S000003436; AllianceGenome:SGD:S000003436; VEuPathDB:YGR204W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; and methylenetetrahydrofolate dehydrogenase (NADP+) activity. Involved in folic acid-containing compound metabolic process and purine nucleobase biosynthetic process. Located in cytoplasm and nucleus. Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; cleft lip; developmental cardiac valvular defect; neural tube defect (multiple); and tetralogy of Fallot. Orthologous to several human genes including MTHFD1 (methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ADE3 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (905934..908774)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Gcn4p is responsible for the basal and inducible expression of the ADE3 gene, while Bas1p is required for its basal expression.
Title: Cooperative regulation of ADE3 transcription by Gcn4p and Bas1p in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables formate-tetrahydrofolate ligase activity	IEA Inferred from Electronic Annotation more info
enables formate-tetrahydrofolate ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables methenyltetrahydrofolate cyclohydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables methenyltetrahydrofolate cyclohydrolase activity	IEA Inferred from Electronic Annotation more info
enables methenyltetrahydrofolate cyclohydrolase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity	IEA Inferred from Electronic Annotation more info
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in L-histidine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in folic acid-containing compound metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in methionine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in one-carbon metabolic process	IEA Inferred from Electronic Annotation more info
involved_in purine nucleobase biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in purine nucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in tetrahydrofolate interconversion	IBA Inferred from Biological aspect of Ancestor more info
involved_in tetrahydrofolate interconversion	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3

NP_011720.3

EC 1.5.1.5

EC 3.5.4.9

EC 6.3.4.3

Cytoplasmic trifunctional enzyme; catalyzes sequential reactions 10-formyl-THF synthetase (EC 6.3.4.3), 5,10-methenyl-THF cyclohydrolase (EC 3.5.4.9), and 5,10-methylene-THF dehydrogenase (EC 1.5.1.5), to supply forms of activated one-carbon units required for biosynthesis of purines, histidine, methionine, pantothenic acid, the formyl group of mitochondrial fMet-tRNAfMet, and the methyl group of thymidylate; null mutation causes auxotrophy for adenine and histidine in rich medium

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

905934..908774

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181333.3 → NP_011720.3 TPA: trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011720.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUY6, P07245

UniProtKB/TrEMBL

A0A8H4C326, A6ZUL4, B5VJE6, C7GRN6, C8Z996, N1P634

Conserved Domains (2) summary

COG0190
Location:4 → 303

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]

pfam01268
Location:322 → 946

FTHFS; Formate--tetrahydrofolate ligase