RAD6 E2 ubiquitin-conjugating protein RAD6 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: RAD6
Gene description: E2 ubiquitin-conjugating protein RAD6
Primary source: FungiDB:YGL058W
Locus tag: YGL058W
See related: SGD:S000003026; AllianceGenome:SGD:S000003026; VEuPathDB:YGL058W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PSO8; UBC2
Summary: Enables proteasome binding activity and ubiquitin-protein transferase activity. Contributes to single-stranded DNA binding activity and single-stranded DNA helicase activity. Involved in several processes, including nucleic acid metabolic process; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein ubiquitination. Located in cytoplasm and nucleus. Part of MUB1-RAD6-UBR2 ubiquitin ligase complex; Rad6-Rad18 complex; and UBR1-RAD6 ubiquitin ligase complex. Colocalizes with chromatin. Human ortholog(s) of this gene implicated in syndromic X-linked intellectual disability Nascimento type. Orthologous to several human genes including UBE2B (ubiquitin conjugating enzyme E2 B). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RAD6 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (393986..394504)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Paf1 complex subunit Rtf1 stimulates H2B ubiquitylation by interacting with the highly conserved N-terminal helix of Rad6.
Title: Paf1 complex subunit Rtf1 stimulates H2B ubiquitylation by interacting with the highly conserved N-terminal helix of Rad6.
Structural basis for the Rad6 activation by the Bre1 N-terminal domain.
Title: Structural basis for the Rad6 activation by the Bre1 N-terminal domain.
Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex.
Title: Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex.
Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126 in helix-3 affect ubiquitination activity and decrease enzyme stability.
Title: Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126 in helix-3 affect ubiquitination activity and decrease enzyme stability.
By docking onto the NCP surface in this distinct orientation, Bre1 positions the Rad6 active site directly over H2B Lys123. The Spt-Ada-Gcn5 acetyltransferase (SAGA) H2B deubiquitinase module competes with Bre1 for binding to the NCP acidic patch, indicating regulatory control. Our study reveals a mechanism that ensures site-specific NCP ubiquitination and fine-tuning of opposing enzymatic activities
Title: Structural mechanism for the recognition and ubiquitination of a single nucleosome residue by Rad6-Bre1.
The histone modification domain of Paf1 complex subunit Rtf1 directly stimulates H2B ubiquitylation through an interaction with Rad6.
Title: The Histone Modification Domain of Paf1 Complex Subunit Rtf1 Directly Stimulates H2B Ubiquitylation through an Interaction with Rad6.
Rad6-Bre1 pathway regulates both telomere length and recombination through its role in histone H2B lysine 123 mono-ubiquitination
Title: Rad6-Bre1-mediated H2B ubiquitination regulates telomere replication by promoting telomere-end resection.
Free ubiquitin interacts weakly with both non-canonical and canonical backside residues of Rad6 and that mutations of non-canonical residues have deleterious effects on Rad6 activity.
Title: Role of a non-canonical surface of Rad6 in ubiquitin conjugating activity.
The authors demonstrate that tombusvirus RNA replication and the mono- and bi-ubiquitination level of p33 is decreased in rad6Delta yeast. However, plasmid-based expression of the Arabidopsis Ubc2p could complement both defects in rad6Delta yeast.
Title: Cellular Ubc2/Rad6 E2 ubiquitin-conjugating enzyme facilitates tombusvirus replication in yeast and plants.
Data show that E3 ligase Bre1 RING domain directs ubiquitin-conjugating enzyme E2 Rad6 to monoubiquitinate histone H2B Lys-123.
Title: Monoubiquitination of histone H2B is intrinsic to the Bre1 RING domain-Rad6 interaction and augmented by a second Rad6-binding site on Bre1.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables proteasome binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin conjugating enzyme activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated transcription termination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ERAD pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to unfolded protein	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in cytoplasm protein quality control by the ubiquitin-proteasome system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in error-free postreplication DNA repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in error-free translesion synthesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in error-prone translesion synthesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic DNA double-strand break formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic G1 DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in regulation of dipeptide transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sno(s)RNA transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sporulation resulting in formation of a cellular spore	IEA Inferred from Electronic Annotation more info
involved_in stress-induced homeostatically regulated protein degradation pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transcription by RNA polymerase II	IPI Inferred from Physical Interaction more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IPI Inferred from Physical Interaction more info	PubMed
involved_in ubiquitin-dependent protein catabolic process via the N-end rule pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of HULC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MUB1-RAD6-UBR2 ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of Rad6-Rad18 complex	IDA Inferred from Direct Assay more info	PubMed
part_of UBR1-RAD6 ubiquitin ligase complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of UBR1-RAD6 ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E2 ubiquitin-conjugating protein RAD6

NP_011457.1

EC 2.3.2.23

Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

393986..394504

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180923.1 → NP_011457.1 TPA: E2 ubiquitin-conjugating protein RAD6 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011457.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU83, P06104

UniProtKB/TrEMBL

A0A6L0YJ92, A6ZUB8, C7GRJ4, C8Z8I9, G2WE38, N1P383

Conserved Domains (1) summary

COG5078
Location:1 → 150

COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]