RPT6 proteasome regulatory particle base subunit RPT6 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPT6
Official Full Name: proteasome regulatory particle base subunit RPT6
Primary source: SGD:S000003016
Locus tag: YGL048C
See related: AllianceGenome:SGD:S000003016; FungiDB:YGL048C; VEuPathDB:YGL048C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CIM3; CRL3; SCB68; SUG1
Summary: Enables protein domain specific binding activity and ubiquitin protein ligase binding activity. Involved in several processes, including nonfunctional rRNA decay; positive regulation of transcription by RNA polymerase II; and proteasome regulatory particle assembly. Located in nucleus and proteasome storage granule. Part of proteasome regulatory particle, base subcomplex. Used to study Alzheimer's disease; amyotrophic lateral sclerosis; cancer; inclusion body myopathy with Paget disease of bone and frontotemporal dementia; and myopathy. Orthologous to human PSMC5 (proteasome 26S subunit, ATPase 5). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See RPT6 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (410069..411286, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

suggest that Rpt6 phosphorylation at S119 may play an important function in proteasome-dependent relief of proteotoxic stress that can be critical in protein aggregation pathologies
Title: Phosphorylation of the 19S regulatory particle ATPase subunit, Rpt6, modifies susceptibility to proteotoxic stress and protein aggregation.
Data show that both ATPases Rpt6 and Rpt3 tails are required for the eviction of Nas6 chaperone upon proteasome assembly.
Title: Proteasome Activation is Mediated via a Functional Switch of the Rpt6 C-terminal Tail Following Chaperone-dependent Assembly.
Data suggest that the proteasome and the TOR signalling pathway synergistically regulate a significant portion of the genome to coordinate cell growth and starvation response.
Title: Synergistic effects of TOR and proteasome pathways on the yeast transcriptome and cell growth.
S. cerevisiae Rpt6's C-terminal domain undergoes dynamic helix-coil transitions enabled by helix-destabilizing glycines within its two most C-terminal alpha helices.
Title: Conformational dynamics of the Rpt6 ATPase in proteasome assembly and Rpn14 binding.
Two alpha-subunits (alpha1 and alpha7) of the 20S proteasome in the rpt6-1 mutant differed from their wild-type counterparts and peptidase activities were found to be lower in the mutant than in the wild-type strain
Title: Functional and biochemical characterization of the 20S proteasome in a yeast temperature-sensitive mutant, rpt6-1.
The non-proteolytic requirement for the proteasomal Sug1 ATPase includes at least the heat and oxidative stress-responsive genes.
Title: The proteasomal ATPase complex is required for stress-induced transcription in yeast.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables proteasome-activating activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein domain specific binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nonfunctional rRNA decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleotide-excision repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of RNA polymerase II transcription preinitiation complex assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of RNA polymerase II transcription preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of macromolecule metabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of proteasomal protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription elongation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome regulatory particle assembly	IEA Inferred from Electronic Annotation more info
involved_in proteasome regulatory particle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of proteasome complex	IEA Inferred from Electronic Annotation more info
part_of proteasome regulatory particle, base subcomplex	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome regulatory particle, base subcomplex	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome regulatory particle, base subcomplex	IEA Inferred from Electronic Annotation more info
located_in proteasome storage granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proteasome regulatory particle base subunit RPT6

NP_011467.1

ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

410069..411286 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180913.1 → NP_011467.1 TPA: proteasome regulatory particle base subunit RPT6 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011467.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU92, Q01939

UniProtKB/TrEMBL

A6ZUU9, B3LIQ7, B5VIS1, C8Z8J6, G2WE48, N1P3L9

Conserved Domains (1) summary

COG1222
Location:12 → 403

RPT1; ATP-dependent 26S proteasome regulatory subunit [Posttranslational modification, protein turnover, chaperones]