ALG13 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ALG13
Primary source: FungiDB:YGL047W
Locus tag: YGL047W
See related: SGD:S000003015; AllianceGenome:SGD:S000003015; VEuPathDB:YGL047W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity. Involved in dolichol-linked oligosaccharide biosynthetic process. Located in cytosol. Is extrinsic component of endoplasmic reticulum membrane. Part of UDP-N-acetylglucosamine transferase complex. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 36. Orthologous to human ALG13 (ALG13 UDP-N-acetylglucosaminyltransferase subunit). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (411552..412160)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The N- and C-termini of Alg14 coordinate recruitment of catalytic Alg7 and Alg13 to the endoplasmic reticulum membrane for initiating lipid-linked oligosaccharide biosynthesis.
Title: Alg14 organizes the formation of a multiglycosyltransferase complex involved in initiation of lipid-linked oligosaccharide biosynthesis.
Alg13 and Alg14 interaction at the C terminus mediates formation of the active UDP-N-acetylglucosamine transferase complex
Title: Interaction between the C termini of Alg13 and Alg14 mediates formation of the active UDP-N-acetylglucosamine transferase complex.
These data lead to the model that proteasomal degradation of excess unassembled Alg13p is an important quality control mechanism that ensures proper protein complex assembly and correct N-linked glycosylation.
Title: Alg13p, the catalytic subunit of the endoplasmic reticulum UDP-GlcNAc glycosyltransferase, is a target for proteasomal degradation.
Titration of Alg13 with both UDP-GlcNAc, the native glycosyl donor, and a paramagnetic mimic, UDP-TEMPO, shows that the interaction of Alg13 with the sugar donor is primarily through the residues in the C-terminal half of the protein.
Title: Solution structure of Alg13: the sugar donor subunit of a yeast N-acetylglucosamine transferase.
Alg13-Alg14 UDP-GlcNAc transferase represents an unprecedented example of a bipartite glycosyltransferase that evolved by both fission and fusion
Title: Membrane topology of the Alg14 endoplasmic reticulum UDP-GlcNAc transferase subunit.
Alg13p associates with Alg14p to a complex forming the active transferase catalyzing the biosynthesis of GlcNAc(2)-PP-Dol
Title: Biosynthesis of lipid-linked oligosaccharides in Saccharomyces cerevisiae: Alg13p and Alg14p form a complex required for the formation of GlcNAc(2)-PP-dolichol.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity	IEA Inferred from Electronic Annotation more info
contributes_to N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables hexosyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in dolichol-linked oligosaccharide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in dolichol-linked oligosaccharide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in dolichol-linked oligosaccharide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of UDP-N-acetylglucosamine transferase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of UDP-N-acetylglucosamine transferase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed

General protein information

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Preferred Names: N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase catalytic subunit ALG13

NP_011468.1

EC 2.4.1.141

Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress; both human homologs ALG13 and ALG14 are required to complement yeast alg13 mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

411552..412160

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180912.1 → NP_011468.1 TPA: N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase catalytic subunit ALG13 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011468.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU93, P53178

UniProtKB/TrEMBL

A6ZUV0, B3LIQ6, B5VIS2, C7GS24, C8Z8J7, G2WE49, N1P5I6

Conserved Domains (1) summary

cl10013
Location:8 → 202

Glycosyltransferase_GTB-type; glycosyltransferase family 1 and related proteins with GTB topology