YPT32 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: YPT32
Primary source: FungiDB:YGL210W
Locus tag: YGL210W
See related: SGD:S000003178; AllianceGenome:SGD:S000003178; VEuPathDB:YGL210W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables GTPase activity. Involved in early endosome to Golgi transport and exocytosis. Located in Golgi apparatus and endosome. Orthologous to several human genes including RAB11A (RAB11A, member RAS oncogene family). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See YPT32 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (93792..94460)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ypt32 suppresses autophagy defects in trs130 and trs65 mutants.
Title: Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae.
the crystal structures of GTP and GDP forms of Ypt32 to understand the molecular basis for Rab function.
Title: The activation cycle of Rab GTPase Ypt32 reveals structural determinants of effector recruitment and GDI binding.
Data show that Gyp1p, a GAP for Ypt1p, specifically interacts with Ypt32p, and that this interaction is important for the localization and stability of Gyp1p.
Title: A Rab GAP cascade defines the boundary between two Rab GTPases on the secretory pathway.
Ypt31/32 play roles in both the formation of trans-Golgi vesicles and their subsequent Myo2-dependent motility
Title: Direct interaction between a myosin V motor and the Rab GTPases Ypt31/32 is required for polarized secretion.
myosin light chain 1 (Mlc1p), required for vesicle delivery at the mother-bud neck during cytokinesis, binds to the Myo2p GTD in a region overlapping that of Ypt32p.
Title: Ypt32p and Mlc1p bind within the vesicle binding region of the class V myosin Myo2p globular tail domain.
Mlc1p/Myo2p interaction is stimulated by a signal that requires Sec2p and activation of Ypt32p
Title: GTP drives myosin light chain 1 interaction with the class V myosin Myo2 IQ motifs via a Sec2 RabGEF-mediated pathway.
Ypt31/32 GTPases regulate the function of Rcy1 in the phosphorylation and/or ubiquitination of proteins that recycle through the Golgi
Title: Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling.
Ypt31p/32p-Rcy1p pathway regulates putative phospholipid translocases to promote formation of vesicles destined for the trans-Golgi network from early endosomes.
Title: Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway.
We propose that Ypt31/32p have a role in regulating the delivery of Chs3p to the plasma membrane and deposition of chitin at the cell surface.
Title: Ypt32p regulates the translocation of Chs3p from an internal pool to the plasma membrane.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in early endosome to Golgi transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IEA Inferred from Electronic Annotation more info
involved_in exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle-mediated transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in cellular bud neck	HDA more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	HDA more info	PubMed
is_active_in recycling endosome	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Rab family GTPase YPT32

NP_011305.1

Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

93792..94460

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181075.1 → NP_011305.1 TPA: Rab family GTPase YPT32 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011305.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTU5, P51996

UniProtKB/TrEMBL

A6ZTX9, B3LHQ6, C7GWY6, C8Z845, G2WDP8, N1P4I1

Conserved Domains (1) summary

cd01868
Location:11 → 175

Rab11_like; Rab GTPase family 11 (Rab11)-like includes Rab11a, Rab11b, and Rab25