NUM1 Num1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NUM1
Official Full Name: Num1p
Primary source: SGD:S000002557
Locus tag: YDR150W
See related: AllianceGenome:SGD:S000002557; FungiDB:YDR150W; VEuPathDB:YDR150W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PAC12
Summary: Enables phosphatidylinositol-4,5-bisphosphate binding activity and tubulin binding activity. Involved in microtubule cytoskeleton organization; mitochondrion organization; and nuclear migration along microtubule. Acts upstream of or within intracellular distribution of mitochondria. Located in cell cortex; cellular bud tip; and endoplasmic reticulum. Is active in mitochondria-associated endoplasmic reticulum membrane contact site. [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See NUM1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (755628..763874)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mitochondria-ER-PM contacts regulate mitochondrial division and PI(4)P distribution.
Title: Mitochondria-ER-PM contacts regulate mitochondrial division and PI(4)P distribution.
Loss of Num1-mediated cortical dynein anchoring negatively impacts respiratory growth.
Title: Loss of Num1-mediated cortical dynein anchoring negatively impacts respiratory growth.
Hierarchical integration of mitochondrial and nuclear positioning pathways by the Num1 EF hand.
Title: Hierarchical integration of mitochondrial and nuclear positioning pathways by the Num1 EF hand.
Biochemical Characterization of the Num1-Mdm36 Complex at the Mitochondria-Plasma Membrane Contact Site.
Title: Biochemical Characterization of the Num1-Mdm36 Complex at the Mitochondria-Plasma Membrane Contact Site.
Cells lacking Scs2/Scs22 performed spindle positioning via MT end capture-shrinkage mechanism, requiring dynein anchorage to an ER- and mitochondria-independent population of Num1, dynein motor activity, and CAP-Gly domain of dynactin Nip100/p150(Glued) subunit. Additionally, a CAAX-targeted Num1 rescued loss of lateral patches and MT sliding in the absence of Scs2/Scs22
Title: Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1.
these findings indicate that dynein activity is directly switched "on" by Num1, which induces Pac1/LIS1 removal.
Title: The dynein cortical anchor Num1 activates dynein motility by relieving Pac1/LIS1-mediated inhibition.
The yeast cell cortical protein Num1 integrates mitochondrial dynamics into cellular architecture.
Title: The yeast cell cortical protein Num1 integrates mitochondrial dynamics into cellular architecture.
Thus, our studies have revealed an additional role for Dnm1p in mitochondrial transmission through its interaction with Num1p, thereby providing a link between mitochondrial division and inheritance.
Title: Yeast mitochondrial division and distribution require the cortical num1 protein.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phospholipid binding	IEA Inferred from Electronic Annotation more info
enables tubulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables tubulin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within intracellular distribution of mitochondria	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within intracellular distribution of mitochondria	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of protein location in cell cortex	IEA Inferred from Electronic Annotation more info
involved_in microtubule cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial fission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion inheritance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitochondrion inheritance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration along microtubule	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IEA Inferred from Electronic Annotation more info
is_active_in cellular bud tip	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondria-associated endoplasmic reticulum membrane contact site	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed

General protein information

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Preferred Names: Num1p

NP_010434.1

Protein required for nuclear migration; component of the mitochondria-ER-cortex-anchor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

755628..763874

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180457.1 → NP_010434.1 TPA: Num1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010434.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSD2, Q00402, Q03767

Conserved Domains (3) summary

pfam09528
Location:641 → 1171

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

pfam00169
Location:2574 → 2682

PH; PH domain

pfam12814
Location:2566 → 2684

Mcp5_PH; Meiotic cell cortex C-terminal pleckstrin homology