SRO7 Rab GTPase-binding protein SRO7 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SRO7
Official Full Name: Rab GTPase-binding protein SRO7
Primary source: SGD:S000006236
Locus tag: YPR032W
See related: AllianceGenome:SGD:S000006236; FungiDB:YPR032W; VEuPathDB:YPR032W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SNI1; SOP1
Summary: Enables SNARE binding activity and small GTPase binding activity. Involved in several processes, including Golgi to plasma membrane transport; exocytosis; and small GTPase-mediated signal transduction. Located in cytosol; mating projection tip; and plasma membrane. Orthologous to several human genes including LLGL2 (LLGL scribble cell polarity complex component 2) and STXBP5L (syntaxin binding protein 5L). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SRO7 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (634123..637224)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Allosteric regulation of exocyst: Discrete activation of tethering by two spatial signals.
Title: Allosteric regulation of exocyst: Discrete activation of tethering by two spatial signals.
Authors describe the identification of a Sec4 binding site on the surface of Sro7 that is contained within a cleft created by the junction of two adjacent beta-propellers that form the core structure of Sro7.
Title: Structural basis for recognition of the Sec4 Rab GTPase by its effector, the Lgl/tomosyn homologue, Sro7.
These results suggested that the tumour suppressor homologue SRO7/SRO77 regulated cell proliferation and yeast colony development via the Rho1-Tor1 pathway.
Title: Sro7 and Sro77, the yeast homologues of the Drosophila lethal giant larvae (Lgl), regulate cell proliferation via the Rho1-Tor1 pathway.
Intracellular traffic in yeast between the Golgi and the cell surface is mediated by vesicular carriers and highly dependent on both Sro7 and Rab GTPase, Sec4.
Title: In vitro reconstitution of Rab GTPase-dependent vesicle clustering by the yeast lethal giant larvae/tomosyn homolog, Sro7.
Data show that vesicles isolated from cells lacking the polarity protein Sro7p contained essentially the same catalogue of proteins but showed a reduced content of a subset of cargo proteins.
Title: Quantitative proteomics of yeast post-Golgi vesicles reveals a discriminating role for Sro7p in protein secretion.
Results characterize the interaction between Sro7 and the type V myosin Myo2 and show that this interaction is important for two distinct aspects of Sro7 function.
Title: Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis.
Blocked exocytic sorting in sro7Delta mutants gives rise to quality control-mediated routing of Ena1p to the vacuole.
Title: The yeast tumor suppressor homologue Sro7p is required for targeting of the sodium pumping ATPase to the cell surface.
crystal structure of Sro7 reveals two seven-bladed WD40 beta-propellers followed by a 60-residue-long 'tail', which is bound to the surface of the amino-terminal propeller
Title: Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables SNARE binding	IPI Inferred from Physical Interaction more info	PubMed
enables myosin II binding	IBA Inferred from Biological aspect of Ancestor more info
enables small GTPase binding	IDA Inferred from Direct Assay more info	PubMed
enables syntaxin binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in Golgi to plasma membrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in Golgi to plasma membrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of cell polarity	IPI Inferred from Physical Interaction more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IEA Inferred from Electronic Annotation more info
involved_in exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in small GTPase-mediated signal transduction	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Rab GTPase-binding protein SRO7

NP_015357.1

Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

634123..637224

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184129.1 → NP_015357.1 TPA: Rab GTPase-binding protein SRO7 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015357.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W442, Q12038

UniProtKB/TrEMBL

C7GQA6, N1NXH9

Conserved Domains (1) summary

pfam08596
Location:548 → 943

Lgl_C; Lethal giant larvae(Lgl) like, C-terminal