EXO1 Rad2 family nuclease EXO1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: EXO1
Official Full Name: Rad2 family nuclease EXO1
Primary source: SGD:S000005559
Locus tag: YOR033C
See related: AllianceGenome:SGD:S000005559; FungiDB:YOR033C; VEuPathDB:YOR033C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DHS1
Summary: Enables 5'-3' exonuclease activity and 5'-flap endonuclease activity. Involved in DNA metabolic process and mitotic G2/M transition checkpoint. Located in cytoplasm and nucleus. Used to study primary ovarian insufficiency. Orthologous to human EXO1 (exonuclease 1). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See EXO1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (392416..394524, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss of Dna2 fidelity results in decreased Exo1-mediated resection at DNA double-strand breaks.
Title: Loss of Dna2 fidelity results in decreased Exo1-mediated resection at DNA double-strand breaks.
Exo1 protects DNA nicks from ligation to promote crossover formation during meiosis.
Title: Exo1 protects DNA nicks from ligation to promote crossover formation during meiosis.
Rad27 and Exo1 function in different excision pathways for mismatch repair in Saccharomyces cerevisiae.
Title: Rad27 and Exo1 function in different excision pathways for mismatch repair in Saccharomyces cerevisiae.
We show here that Exo1 phosphorylation is Dun1-independent and Rad53-dependent in response to DNA damage or dNTP depletion, and in both situations Exo1 is similarly phosphorylated at multiple sites.
Title: Exo1 phosphorylation inhibits exonuclease activity and prevents fork collapse in rad53 mutants independently of the 14-3-3 proteins.
By conditionally depleting lagging-strand nucleases and directly analyzing Okazaki fragments synthesized in vivo in Saccharomyces cerevisiae, we conduct a systematic evaluation of the impact of Rad27, Dna2 and Exo1 on lagging-strand synthesis. We find that Rad27 processes the majority of lagging-strand flaps, with a significant additional contribution from Exo1 but not from Dna2.
Title: Processing of eukaryotic Okazaki fragments by redundant nucleases can be uncoupled from ongoing DNA replication in vivo.
A harmless head-on replication-transcription clash resolution requires the fine-tuning of origin firing and coordination among Sen1, Exo1, Mre11 and Mrc1-Ctf4 complexes.
Title: Dormant origins and fork protection mechanisms rescue sister forks arrested by transcription.
This function requires interaction with the replication clamp, proliferating cell nuclear antigen, facilitating its recruitment to damage sites, and complements the activity of an exonuclease, Exo1, in the processing of post-replicative daughter-strand gaps in preparation for template switching
Title: The helicase Pif1 functions in the template switching pathway of DNA damage bypass.
MRX creates a specific DNA end structure that promotes Exo1 resection activity by facilitating the persistence of this nuclease on the DSB ends, uncovering a novel MRX function in DSB resection.
Title: The MRX complex regulates Exo1 resection activity by altering DNA end structure.
Npl3 participates in DNA double-strand break resection by promoting the proper biogenesis of EXO1 mRNA.
Title: The RNA binding protein Npl3 promotes resection of DNA double-strand breaks by regulating the levels of Exo1.
Exo1-dependent and independent mismatch repair has been described. (Review)
Title: Exonuclease 1-dependent and independent mismatch repair.

Submit: New GeneRIF Correction See all GeneRIFs (40)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 5'-3' DNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-3' DNA exonuclease activity	IEA Inferred from Electronic Annotation more info
enables 5'-3' exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-flap endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-flap endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA nuclease activity	IEA Inferred from Electronic Annotation more info
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA double-strand break processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in gene conversion at mating-type locus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mismatch repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic G2/M transition checkpoint	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomeric 3' overhang formation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Rad2 family nuclease EXO1

NP_014676.1

5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

392416..394524 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183452.1 → NP_014676.1 TPA: Rad2 family nuclease EXO1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014676.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W299, P39875

UniProtKB/TrEMBL

G2WMR4, N1NWU6

Conserved Domains (3) summary

cd09908
Location:215 → 287

H3TH_EXO1; H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease

PTZ00108
Location:267 → 520

PTZ00108; DNA topoisomerase 2-like protein; Provisional

cd09857
Location:1 → 202

PIN_EXO1; FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs