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    MED6 mediator complex subunit 6 [ Homo sapiens (human) ]

    Gene ID: 10001, updated on 2-Nov-2024

    Summary

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    Official Symbol
    MED6provided by HGNC
    Official Full Name
    mediator complex subunit 6provided by HGNC
    Primary source
    HGNC:HGNC:19970
    See related
    Ensembl:ENSG00000133997 MIM:602984; AllianceGenome:HGNC:19970
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    ARC33; NY-REN-28
    Summary
    Enables transcription coactivator activity and transcription coactivator binding activity. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of core mediator complex and mediator complex. [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in lymph node (RPKM 6.4), ovary (RPKM 5.9) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See MED6 in Genome Data Viewer
    Location:
    14q24.2
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 14 NC_000014.9 (70583221..70600655, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 14 NC_060938.1 (64789242..64806677, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 14 NC_000014.8 (71049938..71067372, complement)

    Chromosome 14 - NC_000014.9Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105370556 Neighboring gene ADAM metallopeptidase domain 20 Neighboring gene ADAM metallopeptidase domain 20 pseudogene Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr14:71066874-71068073 Neighboring gene double homeobox A pseudogene 12 Neighboring gene keratin 18 pseudogene 7 Neighboring gene RNA, 7SL, cytoplasmic 77, pseudogene Neighboring gene TTC9 divergent transcript Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8655 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr14:71102515-71103714 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8656 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8657 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5887 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5888 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5889 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr14:71120238-71120824 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5890 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8658 Neighboring gene tetratricopeptide repeat domain 9 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8659 Neighboring gene H3K27ac hESC enhancer GRCh37_chr14:71133100-71133600 Neighboring gene H3K27ac hESC enhancer GRCh37_chr14:71133601-71134101

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription. Sun X, et al. Mol Cell, 1998 Aug. PMID 9734358
    2. The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator. Yang F, et al. Proc Natl Acad Sci U S A, 2004 Feb 24. PMID 14983011, Free PMC Article
    3. Characterization of mediator complexes from HeLa cell nuclear extract. Wang G, et al. Mol Cell Biol, 2001 Jul. PMID 11416138, Free PMC Article
    4. Mediator links epigenetic silencing of neuronal gene expression with x-linked mental retardation. Ding N, et al. Mol Cell, 2008 Aug 8. PMID 18691967, Free PMC Article
    5. Mediator CDK subunits are platforms for interactions with various chromatin regulatory complexes. Fukasawa R, et al. J Biochem, 2012 Sep. PMID 22668559

    See all (84) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Replication interactions

    Interaction Pubs
    Knockdown of mediator complex subunit 6 (MED6) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed
    Knockdown of mediator complex subunit 6 (MED6) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed
    Knockdown of mediator complex subunit 6 (MED6) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat Knockdown of MED6 by siRNAs inhibits HIV LTR-beta-gal activation in the Tat transactivation assay PubMed
    tat The interaction of HIV-1 Tat with MED21 hypothetically induces the binding of Tat to MED6 PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription coactivator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coactivator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transcription coactivator binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin protein ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription elongation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in somatic stem cell population maintenance IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of core mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of core mediator complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of mediator complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of ubiquitin ligase complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    mediator of RNA polymerase II transcription subunit 6
    Names
    CTD-2540L5.5
    activator-recruited cofactor 33 kDa component
    renal carcinoma antigen NY-REN-28

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001284209.2 → NP_001271138.1  mediator of RNA polymerase II transcription subunit 6 isoform 1

      See identical proteins and their annotated locations for NP_001271138.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.
      Source sequence(s)
      AK300460, BM999935, DA869309
      Consensus CDS
      CCDS61484.1
      UniProtKB/TrEMBL
      A8K3N2
      Related
      ENSP00000413343.2, ENST00000430055.6
      Conserved Domains (1) summary
      pfam04934
      Location:13 → 146
      Med6; MED6 mediator sub complex component

    2. NM_001284210.2 → NP_001271139.1  mediator of RNA polymerase II transcription subunit 6 isoform 3

      See identical proteins and their annotated locations for NP_001271139.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) uses an alternate splice site and lacks an alternate exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1.
      Source sequence(s)
      AK300460, AK304361, BM999935, DA869309
      Consensus CDS
      CCDS61483.1
      UniProtKB/Swiss-Prot
      O75586
      Related
      ENSP00000394502.2, ENST00000440435.2
      Conserved Domains (1) summary
      pfam04934
      Location:13 → 139
      Med6; MED6 mediator sub complex component

    3. NM_001284211.2 → NP_001271140.1  mediator of RNA polymerase II transcription subunit 6 isoform 4

      See identical proteins and their annotated locations for NP_001271140.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) uses an alternate splice site and differs in the 3' exon structure, which results in a frameshift, compared to variant 1. The encoded isoform (4) is longer and has a distinct C-terminus, compared to isoform 1.
      Source sequence(s)
      AK300460, BM802752, BM999935, DA869309, HY064772
      Consensus CDS
      CCDS73649.1
      UniProtKB/TrEMBL
      A0A087WYL7, A8K3N2
      Related
      ENSP00000481920.1, ENST00000615788.4
      Conserved Domains (1) summary
      pfam04934
      Location:13 → 139
      Med6; MED6 mediator sub complex component

    4. NM_005466.4 → NP_005457.2  mediator of RNA polymerase II transcription subunit 6 isoform 2

      See identical proteins and their annotated locations for NP_005457.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
      Source sequence(s)
      AL357153, BC004106, BM999935, DA869309, HY064772
      Consensus CDS
      CCDS9805.1
      UniProtKB/Swiss-Prot
      B4DU17, B4E2P0, O15401, O75586, Q53FE3, Q53HJ3, Q6FHQ4, Q9BTH1, Q9UHL1
      UniProtKB/TrEMBL
      A8K3N2
      Related
      ENSP00000256379.5, ENST00000256379.10
      Conserved Domains (1) summary
      pfam04934
      Location:13 → 140
      Med6; MED6 mediator sub complex component

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000014.9 Reference GRCh38.p14 Primary Assembly

      Range
      70583221..70600655 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060938.1 Alternate T2T-CHM13v2.0

      Range
      64789242..64806677 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O75586.2 GenPept UniProtKB/Swiss-Prot:O75586

    Gene LinkOut

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