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    AGO1 argonaute RISC component 1 [ Homo sapiens (human) ]

    Gene ID: 26523, updated on 4-Feb-2024

    Summary

    Official Symbol
    AGO1provided by HGNC
    Official Full Name
    argonaute RISC component 1provided by HGNC
    Primary source
    HGNC:HGNC:3262
    See related
    Ensembl:ENSG00000092847 MIM:606228; AllianceGenome:HGNC:3262
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    Q99; EIF2C; hAgo1; EIF2C1; GERP95; NEDLBAS
    Summary
    This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
    Expression
    Ubiquitous expression in skin (RPKM 4.5), brain (RPKM 3.9) and 25 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See AGO1 in Genome Data Viewer
    Location:
    1p34.3
    Exon count:
    20
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (35869761..35930532)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (35732654..35793426)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (36335362..36396133)

    Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene Sharpr-MPRA regulatory region 10959 Neighboring gene ReSE screen-validated silencer GRCh37_chr1:36191359-36191542 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:36204140-36205339 Neighboring gene claspin Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 646 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 737 Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:36272791-36273290 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 647 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 648 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 738 Neighboring gene argonaute RISC component 4 Neighboring gene ReSE screen-validated silencer GRCh37_chr1:36315018-36315170 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 649 Neighboring gene OCT4-H3K27ac hESC enhancer GRCh37_chr1:36351383-36352022 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 739 Neighboring gene CFAP97 pseudogene 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 740 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr1:36359789-36360988 Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:36396322-36396845 Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:36396846-36397370 Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:36397371-36397893 Neighboring gene argonaute RISC catalytic component 3 Neighboring gene NANOG hESC enhancer GRCh37_chr1:36474078-36474579 Neighboring gene uncharacterized LOC105378647 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 741 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr1:36499894-36501093 Neighboring gene magnesium transporter 1 pseudogene

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Protein interactions

    Protein Gene Interaction Pubs
    Pr55(Gag) gag HIV-1 Gag co-immunoprecipitates with endogenous AGO1 in 293T cells PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • DKFZp686M13167

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables RNA binding HDA PubMed 
    enables RNA binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    NOT enables RNA endonuclease activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables RNA polymerase II complex binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables core promoter sequence-specific DNA binding IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    enables double-stranded RNA binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables miRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables miRNA binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables single-stranded RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables single-stranded RNA binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in P-body IEA
    Inferred from Electronic Annotation
    more info
     
    part_of RISC complex IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    part_of RISC complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of RISC-loading complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in cytoplasmic ribonucleoprotein granule IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytoplasmic ribonucleoprotein granule IDA
    Inferred from Direct Assay
    more info
     
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
     
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in nucleus IC
    Inferred by Curator
    more info
    PubMed 

    General protein information

    Preferred Names
    protein argonaute-1
    Names
    Golgi Endoplasmic Reticulum protein 95 kDa
    argonaute 1, RISC catalytic component
    argonaute RISC catalytic component 1
    argonaute1
    eIF-2C 1
    eIF2C 1
    eukaryotic translation initiation factor 2C, 1
    putative RNA-binding protein Q99

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001317122.2NP_001304051.1  protein argonaute-1 isoform 1x

      Status: REVIEWED

      Description
      Transcript Variant: Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'.
      Source sequence(s)
      AF093097, AK314148, AL139286, BC063275, CR936823, HY052874
      Consensus CDS
      CCDS90915.1
      UniProtKB/TrEMBL
      A0A6I8PTZ8, B2RAD8
      Related
      ENSP00000501372.1, ENST00000674426.1
      Conserved Domains (5) summary
      PLN03202
      Location:26856
      PLN03202; protein argonaute; Provisional
      cd02846
      Location:225345
      PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
      cd04657
      Location:390815
      Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
      pfam08699
      Location:175224
      ArgoL1; Argonaute linker 1 domain
      pfam16486
      Location:26164
      ArgoN; N-terminal domain of argonaute
    2. NM_001317123.2NP_001304052.1  protein argonaute-1 isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) contains an alternate 5' terminal exon, which results in translation initiation from an in-frame, downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.
      Source sequence(s)
      AF093097, AK001622, AK314148, AL139286, CR936823
      Consensus CDS
      CCDS81300.1
      UniProtKB/TrEMBL
      B3KME0, Q5TA58
      Related
      ENSP00000362302.1, ENST00000373206.5
      Conserved Domains (4) summary
      cd02846
      Location:150270
      PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
      cd04657
      Location:315740
      Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
      pfam08699
      Location:99149
      ArgoL1; Argonaute linker 1 domain
      pfam16486
      Location:1589
      ArgoN; N-terminal domain of argonaute
    3. NM_012199.5NP_036331.1  protein argonaute-1 isoform 1

      See identical proteins and their annotated locations for NP_036331.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1).
      Source sequence(s)
      AF093097, AK314148, AL139286, BC063275, CR936823, HY052874
      Consensus CDS
      CCDS398.1
      UniProtKB/Swiss-Prot
      Q5TA57, Q6P4S0, Q9UL18
      UniProtKB/TrEMBL
      B2RAD8
      Related
      ENSP00000362300.4, ENST00000373204.6
      Conserved Domains (4) summary
      cd02846
      Location:225345
      PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
      cd04657
      Location:390815
      Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
      pfam08699
      Location:174224
      ArgoL1; Argonaute linker 1 domain
      pfam16486
      Location:34164
      ArgoN; N-terminal domain of argonaute

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

      Range
      35869761..35930532
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011541236.3XP_011539538.1  protein argonaute-1 isoform X1

      See identical proteins and their annotated locations for XP_011539538.1

      UniProtKB/TrEMBL
      B2RAD8
      Conserved Domains (4) summary
      cd02846
      Location:225345
      PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
      cd04657
      Location:390818
      Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
      pfam08699
      Location:174224
      ArgoL1; Argonaute linker 1 domain
      pfam16486
      Location:34164
      ArgoN; N-terminal domain of argonaute

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060925.1 Alternate T2T-CHM13v2.0

      Range
      35732654..35793426
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054335969.1XP_054191944.1  protein argonaute-1 isoform X1

      UniProtKB/TrEMBL
      B2RAD8