RHNO1 RAD9-HUS1-RAD1 interacting nuclear orphan 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: RHNO1provided by HGNC
Official Full Name: RAD9-HUS1-RAD1 interacting nuclear orphan 1provided by HGNC
Primary source: HGNC:HGNC:28206
See related: Ensembl:ENSG00000171792 MIM:614085; AllianceGenome:HGNC:28206
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: RHINO; C12orf32; HKMT1188
Summary: Enables chromatin-protein adaptor activity. Involved in several processes, including DNA repair; cellular response to radiation; and regulation of cell cycle process. Located in chromosome and nucleus. Is active in chromatin and site of double-strand break. [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in thyroid (RPKM 7.6), lymph node (RPKM 7.3) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 12p13.33

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	12	NC_000012.12 (2876265..2889524)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	12	NC_060936.1 (2882276..2895466)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	12	NC_000012.11 (2985431..2998690)

Chromosome 12 - NC_000012.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural basis for intra- and intermolecular interactions on RAD9 subunit of 9-1-1 checkpoint clamp implies functional 9-1-1 regulation by RHINO.
Title: Structural basis for intra- and intermolecular interactions on RAD9 subunit of 9-1-1 checkpoint clamp implies functional 9-1-1 regulation by RHINO.
RHNO1 disruption inhibits cell proliferation and induces mitochondrial apoptosis via PI3K/Akt pathway in hepatocellular carcinoma.
Title: RHNO1 disruption inhibits cell proliferation and induces mitochondrial apoptosis via PI3K/Akt pathway in hepatocellular carcinoma.
RHINO directs MMEJ to repair DNA breaks in mitosis.
Title: RHINO directs MMEJ to repair DNA breaks in mitosis.
Co-regulation and function of FOXM1/RHNO1 bidirectional genes in cancer.
Title: Co-regulation and function of FOXM1/RHNO1 bidirectional genes in cancer.
Knockdown of RHINO in human cells partially abrogated ATR-Chk1 kinase signaling following UV irradiation but did not impact the loading of Rad9-Hus1-Rad1 complex on chromatin or the association of Rad9-Hus1-Rad1 complex with TopBP1.
Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling.
Germline variation in the RHINO gene is unlikely to influence inherited susceptibility to breast cancer.
Title: Evaluation of the RHINO gene for breast cancer predisposition in Finnish breast cancer families.
We suggest that RHINO functions together with the 9-1-1 complex and TopBP1 to fully activate ATR (ataxia telangiectasia and Rad3-related.
Title: A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.
Identified C12orf32 to be significantly up-regulated in the great majority of clinical breast cancer specimens.
Title: Involvement of C12orf32 overexpression in breast carcinogenesis.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH46379 (e-PCR)
- UniSTS:87575

D11S2560 (e-PCR)
- UniSTS:37928

STS-N62451 (e-PCR)
- UniSTS:74684

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables chromatin-protein adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA damage checkpoint signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to UV	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to ionizing radiation	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via alternative nonhomologous end joining	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of G0 to G1 transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to site of double-strand break	IDA Inferred from Direct Assay more info	PubMed
involved_in recombinational repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in recombinational repair	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in chromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in chromosome	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: RAD9, HUS1, RAD1-interacting nuclear orphan protein 1

Names: Rad9, Rad1, Hus1 interacting nuclear orphan; rAD9, RAD1, HUS1-interacting nuclear orphan protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001252499.3 → NP_001239428.1 RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1

See identical proteins and their annotated locations for NP_001239428.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1, 4 and 5 all encode isoform 1.

Source sequence(s)

AB073599, AI631627

Consensus CDS

CCDS8518.1

UniProtKB/Swiss-Prot

B7Z989, Q9BSD3

Related

ENSP00000438590.1, ENST00000489288.7

Conserved Domains (1) summary

pfam15319
Location:1 → 236

RHINO; RAD9, RAD1, HUS1-interacting nuclear orphan protein
NM_001252500.3 → NP_001239429.1 RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 2

See identical proteins and their annotated locations for NP_001239429.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in a shorter isoform (2), compared to isoform 1.

Source sequence(s)

AB073599, AI631627, AK304613

Consensus CDS

CCDS58199.1

UniProtKB/Swiss-Prot

Q9BSD3

Related

ENSP00000438828.1, ENST00000461997.5

Conserved Domains (1) summary

pfam15319
Location:1 → 222

RHINO; RAD9, RAD1, HUS1-interacting nuclear orphan protein
NM_001257097.2 → NP_001244026.1 RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1

See identical proteins and their annotated locations for NP_001244026.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 4 and 5 all encode isoform 1.

Source sequence(s)

AB073599, AI631627, DA749974

Consensus CDS

CCDS8518.1

UniProtKB/Swiss-Prot

B7Z989, Q9BSD3

Related

ENSP00000479598.1, ENST00000618250.4

Conserved Domains (1) summary

pfam15319
Location:1 → 236

RHINO; RAD9, RAD1, HUS1-interacting nuclear orphan protein
NM_001257098.2 → NP_001244027.1 RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1

See identical proteins and their annotated locations for NP_001244027.1

Status: VALIDATED

Description

Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1, 4 and 5 all encode isoform 1.

Source sequence(s)

AB073599, AI631627, DA679606

Consensus CDS

CCDS8518.1

UniProtKB/Swiss-Prot

B7Z989, Q9BSD3

Conserved Domains (1) summary

pfam15319
Location:1 → 236

RHINO; RAD9, RAD1, HUS1-interacting nuclear orphan protein

RNA

NR_027365.3 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an internal exon, compared to variant 1. This variant is represented as non-coding because it lacks a significant portion of the coding region, including the translational start codon, as found in variant 1.

Source sequence(s)

AI631627, AK021945

Related

ENST00000464682.2
NR_046432.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (6) includes an additional internal exon, compared to variant 1. This variant is represented as non-coding because use of the expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AB073599, AC005911, AI631627, DA378594
NR_046433.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (7) uses an alternate 5' exon and lacks an internal exon, compared to variant 1. This variant is represented as non-coding because it lacks a significant portion of the coding region, including the translational start codon, as found in variant 1.

Source sequence(s)

AI631627, AK021945, BM847461