CENPN centromere protein N [Homo sapiens (human)] - Gene

Summary

Official Symbol: CENPNprovided by HGNC
Official Full Name: centromere protein Nprovided by HGNC
Primary source: HGNC:HGNC:30873
See related: Ensembl:ENSG00000166451 MIM:611509; AllianceGenome:HGNC:30873
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: BM039; CENP-N; ICEN32; C16orf60
Summary: The protein encoded by this gene forms part of the nucleosome-associated complex and is important for kinetochore assembly. It is bound to kinetochores during S phase and G2 and recruits other proteins to the centromere. Pseudogenes of this gene are located on chromosome 2. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]
Expression: Ubiquitous expression in prostate (RPKM 4.4), bone marrow (RPKM 3.9) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 16q23.2

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	16	NC_000016.10 (81007214..81033107)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	16	NC_060940.1 (87068448..87094631)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	16	NC_000016.9 (81040819..81066712)

Chromosome 16 - NC_000016.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dynamic phosphorylation of CENP-N by CDK1 guides accurate chromosome segregation in mitosis.
Title: Dynamic phosphorylation of CENP-N by CDK1 guides accurate chromosome segregation in mitosis.
Clinical implications and immune features of CENPN in breast cancer.
Title: Clinical implications and immune features of CENPN in breast cancer.
Knockdown of CENPN Inhibits Glucose Metabolism and Induces G1 Arrest in Esophageal Cancer Cells by Regulating PI3K/AKT Signaling Pathway.
Title: Knockdown of CENPN Inhibits Glucose Metabolism and Induces G1 Arrest in Esophageal Cancer Cells by Regulating PI3K/AKT Signaling Pathway.
Centromere protein N promotes lung adenocarcinoma progression by activating PI3K/AKT signaling pathway.
Title: Centromere protein N promotes lung adenocarcinoma progression by activating PI3K/AKT signaling pathway.
CENP-N promotes the compaction of centromeric chromatin.
Title: CENP-N promotes the compaction of centromeric chromatin.
The Elusive Structure of Centro-Chromatin: Molecular Order or Dynamic Heterogenetity?
Title: The Elusive Structure of Centro-Chromatin: Molecular Order or Dynamic Heterogenetity?
CENP-N stabilizes CENP-A nucleosomes alone and additively with CENP-C in vitro. However, removal of CENP-C and CENP-N from cells, or mutating CENP-A so that it no longer interacts with CENP-C or CENP-N, had no effect on centromeric CENP-A stability in vivo. Thus, the stability of CENP-A nucleosomes in chromatin does not arise solely from its interactions with CENP-C or CENP-N.
Title: Constitutive centromere-associated network contacts confer differential stability on CENP-A nucleosomes in vitro and in the cell.
Collectively, these studies clarify how CENP-N and CENP-C decode and stabilize the non-canonical CENP-A nucleosome to enforce epigenetic centromere specification and kinetochore assembly.
Title: Decoding the centromeric nucleosome through CENP-N.
this study reports cryo-electron microscopy (cryo-EM), biophysical, biochemical, and cell biological studies of the interaction between the CENP-A nucleosome and CENP-N.
Title: Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N.
Study demonstrate that the CENP-L-N complex plays a crucial role at the core of the 16-subunit Constitutive Centromere-Associated Network through interactions with CENP-C and CENP-H-I-KM.
Title: The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork of Interactions at the Centromere-Kinetochore Interface.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D8S2279 (e-PCR)
- UniSTS:473907

D3S587 (e-PCR)
- UniSTS:148553

D11S2687 (e-PCR)
- UniSTS:151912

D1S1423 (e-PCR)
- UniSTS:149619

L18441 (e-PCR)
- UniSTS:71348

RH99185 (e-PCR)
- UniSTS:92881

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in CENP-A containing chromatin assembly	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	TAS Traceable Author Statement more info
part_of inner kinetochore	IPI Inferred from Physical Interaction more info	PubMed
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: centromere protein N

Names: interphase centromere complex protein 32

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001100624.3 → NP_001094094.2 centromere protein N isoform 2

See identical proteins and their annotated locations for NP_001094094.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate 3' terminal exon compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC092718, AK023669, BC039021, HY167728

Consensus CDS

CCDS42200.1

UniProtKB/Swiss-Prot

A8MZE6, B3KN53, B4DJD1, B4DPY7, C9JJM5, D3DUK8, E7ES30, E7ETS3, Q96H22, Q9NZ83

Related

ENSP00000305608.5, ENST00000305850.10

Conserved Domains (1) summary

pfam05238
Location:3 → 339

CENP-N; Kinetochore protein CHL4 like
NM_001100625.3 → NP_001094095.2 centromere protein N isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AK023669, AK026313, HY374758

Consensus CDS

CCDS42199.1

UniProtKB/Swiss-Prot

Q96H22

Related

ENSP00000377007.3, ENST00000393335.7

Conserved Domains (1) summary

pfam05238
Location:3 → 315

CENP-N; Kinetochore protein CHL4 like
NM_001270473.2 → NP_001257402.1 centromere protein N isoform 4

Status: REVIEWED

Description

Transcript Variant: This variant (4) lacks an exon in the coding region and uses an alternate 3' terminal exon compared to variant 1. The resulting protein (isoform 4) is shorter than isoform 1.

Source sequence(s)

AC092718, AK023669, AK296024, BC039021

Consensus CDS

CCDS58483.1

UniProtKB/Swiss-Prot

Q96H22

Related

ENSP00000395235.3, ENST00000439957.7

Conserved Domains (1) summary

pfam05238
Location:3 → 319

CENP-N; Kinetochore protein CHL4 like
NM_001270474.2 → NP_001257403.1 centromere protein N isoform 5

Status: REVIEWED

Description

Transcript Variant: This variant (5) lacks an exon in the coding region and uses an alternate 3' terminal exon compared to variant 1. The resulting protein (isoform 5) is shorter than isoform 1.

Source sequence(s)

AC092718, AK023669, AK298554, BC039021, HY363272

Consensus CDS

CCDS58482.1

UniProtKB/Swiss-Prot

Q96H22

Related

ENSP00000393991.2, ENST00000428963.6

Conserved Domains (1) summary

pfam05238
Location:3 → 305

CENP-N; Kinetochore protein CHL4 like
NM_018455.6 → NP_060925.2 centromere protein N isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks several exons in the 3' coding region and uses an alternate 3' terminal exon, compared to variant 1. The resulting protein (isoform 3) has a shorter and distinct C-terminus, compared to isoform 1.

Source sequence(s)

BC007334, BQ013821, HY167728

Consensus CDS

CCDS10931.1

UniProtKB/TrEMBL

H3BMC7

Related

ENSP00000299572.5, ENST00000299572.9

Conserved Domains (1) summary

pfam05238
Location:3 → 165

CENP-N; Kinetochore protein CHL4 like