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    RNMT RNA guanine-7 methyltransferase [ Homo sapiens (human) ]

    Gene ID: 8731, updated on 2-May-2024

    Summary

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    Official Symbol
    RNMTprovided by HGNC
    Official Full Name
    RNA guanine-7 methyltransferaseprovided by HGNC
    Primary source
    HGNC:HGNC:10075
    See related
    Ensembl:ENSG00000101654 MIM:603514; AllianceGenome:HGNC:10075
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    MET; Met; CMT1; cm1p; hMet; CMT1c; hCMT1; RG7MT1; N7-MTase
    Summary
    Enables RNA binding activity and mRNA (guanine-N7-)-methyltransferase activity. Involved in 7-methylguanosine mRNA capping. Located in fibrillar center and nucleoplasm. Part of mRNA cap binding activity complex; mRNA cap methyltransferase complex; and receptor complex. [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in brain (RPKM 9.8), thyroid (RPKM 9.0) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See RNMT in Genome Data Viewer
    Location:
    18p11.21
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 18 NC_000018.10 (13726673..13764556)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 18 NC_060942.1 (13888989..13926829)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 18 NC_000018.9 (13726672..13764555)

    Chromosome 18 - NC_000018.10Genomic Context describing neighboring genes Neighboring gene low density lipoprotein receptor class A domain containing 4 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13125 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13613273-13613792 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13613793-13614310 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13126 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13127 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13616337-13616838 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13623209-13623708 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13626114-13626614 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13649057-13649558 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13649559-13650058 Neighboring gene microRNA 4526 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr18:13672949-13674148 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr18:13675367-13676566 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:13688247-13688792 Neighboring gene family with sequence similarity 210 member A Neighboring gene H3K27ac hESC enhancer GRCh37_chr18:13725735-13726660 Neighboring gene RNA, 7SL, cytoplasmic 362, pseudogene Neighboring gene H3K27ac hESC enhancer GRCh37_chr18:13775047-13775546 Neighboring gene ReSE screen-validated silencer GRCh37_chr18:13823817-13823997 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9335 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9336 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13834952-13835452 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13872168-13873006 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr18:13876434-13877404 Neighboring gene OCT4-NANOG-H3K4me1 hESC enhancer GRCh37_chr18:13878605-13879337 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr18:13887335-13887847 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9337 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9338 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13129 Neighboring gene melanocortin 5 receptor Neighboring gene melanocortin 2 receptor

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E. Osborne MJ, et al. J Mol Biol, 2022 Mar 15. PMID 35026230, Free PMC Article
    2. RNA guanine-7 methyltransferase catalyzes the methylation of cytoplasmically recapped RNAs. Trotman JB, et al. Nucleic Acids Res, 2017 Oct 13. PMID 28981715, Free PMC Article
    3. Human cap methyltransferase (RNMT) N-terminal non-catalytic domain mediates recruitment to transcription initiation sites. Aregger M, et al. Biochem J, 2013 Oct 1. PMID 23863084, Free PMC Article
    4. Human mRNA cap methyltransferase: alternative nuclear localization signal motifs ensure nuclear localization required for viability. Shafer B, et al. Mol Cell Biol, 2005 Apr. PMID 15767670, Free PMC Article
    5. Post-transcriptional modifications of mRNA. Purification and characterization of cap I and cap II RNA (nucleoside-2'-)-methyltransferases from HeLa cells. Langberg SR, et al. J Biol Chem, 1981 Oct 10. PMID 7275966

    See all (67) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Kinetic characterization of human mRNA guanine-N7 methyltransferase.
      Title: Kinetic characterization of human mRNA guanine-N7 methyltransferase.
    2. Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.
      Title: Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.
    3. Data show that most of the cell lines which exhibited enhanced dependency on RNA guanine-7 methyltransferase (RNMT) harboured oncogenic mutations in phosphatidylinositol 3-kinase catalytic 110-KD alpha (PI3Kalpha).
      Title: Oncogenic PIK3CA mutations increase dependency on the mRNA cap methyltransferase, RNMT, in breast cancer cells.
    4. Mechanism of allosteric activation of human RNMT by RAM has been reported.
      Title: Mechanism of allosteric activation of human mRNA cap methyltransferase (RNMT) by RAM: insights from accelerated molecular dynamics simulations.
    5. These findings suggest that multiple interactions among RNMT-RAM, RNA Pol II factors, and RNA along the transcription unit stimulate transcription.
      Title: mRNA Cap Methyltransferase, RNMT-RAM, Promotes RNA Pol II-Dependent Transcription.
    6. Results identify a cytoplasmic pool of RNMT which is a component of the cytoplasmic capping complex, and demonstrate its function as the methyltransferase that catalyzes the final maturation step in cap homeostasis.
      Title: RNA guanine-7 methyltransferase catalyzes the methylation of cytoplasmically recapped RNAs.
    7. MYC promotes mRNA cap methylation and protein production of Wnt/beta-catenin signaling transcripts through recruitment of cyclin-dependent kinase 7 (CDK7) and consequently RNMT to gene promoters.
      Title: MYC Mediates mRNA Cap Methylation of Canonical Wnt/β-Catenin Signaling Transcripts By Recruiting CDK7 and RNA Methyltransferase.
    8. RNMT-RAM complex coordinates mRNA processing with ribosome production.
      Title: c-Myc co-ordinates mRNA cap methylation and ribosomal RNA production.
    9. CDK1-Cyclin B1 activates RNMT, coordinating mRNA cap methylation with G1 phase transcription.
      Title: CDK1-Cyclin B1 Activates RNMT, Coordinating mRNA Cap Methylation with G1 Phase Transcription.
    10. High RNMT expression is associated with liver cancer.
      Title: Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • KIAA0398, DKFZp686H1252

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables RNA binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables mRNA 5'-cap (guanine-N7-)-methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables mRNA 5'-cap (guanine-N7-)-methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables mRNA 5'-cap (guanine-N7-)-methyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables mRNA 5'-cap (guanine-N7-)-methyltransferase activity TAS
    Traceable Author Statement
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in 7-methylguanosine mRNA capping IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in 7-methylguanosine mRNA capping IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in 7-methylguanosine mRNA capping IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in 7-methylguanosine mRNA capping TAS
    Traceable Author Statement
    more info
    		  
    involved_in RNA 5'-cap (guanine-N7)-methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cellular response to leukemia inhibitory factor IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in fibrillar center IDA
    Inferred from Direct Assay
    more info
    		  
    part_of mRNA cap methyltransferase RNMT:RAMAC complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of mRNA capping enzyme complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of receptor complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    mRNA cap guanine-N7 methyltransferase
    Names
    RNA (guanine-7-) methyltransferase
    hcm1p
    mRNA (guanine-7-)methyltransferase
    mRNA (guanine-N(7)-)-methyltransferase
    mRNA cap methyltransferase
    NP_001295192.1
    NP_001365061.1
    NP_001365063.1
    NP_001365064.1
    NP_003790.1
    XP_011524051.1
    XP_011524052.1
    XP_011524053.1
    XP_011524055.1
    XP_047293863.1
    XP_047293864.1
    XP_054175275.1
    XP_054175276.1
    XP_054175277.1
    XP_054175278.1
    XP_054175279.1
    XP_054175280.1

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001308263.2 → NP_001295192.1  mRNA cap guanine-N7 methyltransferase isoform 1

      See identical proteins and their annotated locations for NP_001295192.1

      Status: VALIDATED

      Source sequence(s)
      AB022605, AK292561, AP001525, DA634865
      Consensus CDS
      CCDS77156.1
      UniProtKB/TrEMBL
      A8K946
      Related
      ENSP00000466111.1, ENST00000592764.5
      Conserved Domains (1) summary
      pfam03291
      Location:136 → 466
      Pox_MCEL; mRNA capping enzyme

    2. NM_001378132.1 → NP_001365061.1  mRNA cap guanine-N7 methyltransferase isoform 3

      Status: VALIDATED

      Source sequence(s)
      AP001525
      Consensus CDS
      CCDS92440.1
      UniProtKB/TrEMBL
      K7EP06
      Related
      ENSP00000467171.1, ENST00000588457.5
      Conserved Domains (1) summary
      cl17173
      Location:1 → 297
      AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...

    3. NM_001378134.1 → NP_001365063.1  mRNA cap guanine-N7 methyltransferase isoform 1

      Status: VALIDATED

      Source sequence(s)
      AP001525
      Consensus CDS
      CCDS77156.1
      UniProtKB/TrEMBL
      A8K946
      Conserved Domains (1) summary
      pfam03291
      Location:136 → 466
      Pox_MCEL; mRNA capping enzyme

    4. NM_001378135.1 → NP_001365064.1  mRNA cap guanine-N7 methyltransferase isoform 2

      Status: VALIDATED

      Source sequence(s)
      AP001525
      Consensus CDS
      CCDS11867.1
      UniProtKB/Swiss-Prot
      B0YJ90, D3DUJ5, O43148, O94996, Q9UIJ9
      UniProtKB/TrEMBL
      A8K946
      Related
      ENSP00000446426.1, ENST00000543302.6
      Conserved Domains (1) summary
      pfam03291
      Location:136 → 475
      Pox_MCEL; mRNA capping enzyme

    5. NM_003799.3 → NP_003790.1  mRNA cap guanine-N7 methyltransferase isoform 2

      See identical proteins and their annotated locations for NP_003790.1

      Status: VALIDATED

      Source sequence(s)
      AB022605, AF067791, AP001525, DA634865
      Consensus CDS
      CCDS11867.1
      UniProtKB/Swiss-Prot
      B0YJ90, D3DUJ5, O43148, O94996, Q9UIJ9
      UniProtKB/TrEMBL
      A8K946
      Related
      ENSP00000372804.2, ENST00000383314.7
      Conserved Domains (1) summary
      pfam03291
      Location:136 → 475
      Pox_MCEL; mRNA capping enzyme

    RNA

    1. NR_165427.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AP001525

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000018.10 Reference GRCh38.p14 Primary Assembly

      Range
      13726673..13764556
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047437908.1 → XP_047293864.1  mRNA cap guanine-N7 methyltransferase isoform X3

      UniProtKB/Swiss-Prot
      B0YJ90, D3DUJ5, O43148, O94996, Q9UIJ9

    2. XM_047437907.1 → XP_047293863.1  mRNA cap guanine-N7 methyltransferase isoform X2

    3. XM_011525749.3 → XP_011524051.1  mRNA cap guanine-N7 methyltransferase isoform X1

      See identical proteins and their annotated locations for XP_011524051.1

      UniProtKB/TrEMBL
      A8K946
      Conserved Domains (2) summary
      COG0500
      Location:196 → 414
      SmtA; SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only]
      pfam03291
      Location:136 → 468
      Pox_MCEL; mRNA capping enzyme

    4. XM_011525751.2 → XP_011524053.1  mRNA cap guanine-N7 methyltransferase isoform X1

      See identical proteins and their annotated locations for XP_011524053.1

      UniProtKB/TrEMBL
      A8K946
      Conserved Domains (2) summary
      COG0500
      Location:196 → 414
      SmtA; SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only]
      pfam03291
      Location:136 → 468
      Pox_MCEL; mRNA capping enzyme

    5. XM_011525750.3 → XP_011524052.1  mRNA cap guanine-N7 methyltransferase isoform X1

      See identical proteins and their annotated locations for XP_011524052.1

      UniProtKB/TrEMBL
      A8K946
      Conserved Domains (2) summary
      COG0500
      Location:196 → 414
      SmtA; SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only]
      pfam03291
      Location:136 → 468
      Pox_MCEL; mRNA capping enzyme

    6. XM_011525753.3 → XP_011524055.1  mRNA cap guanine-N7 methyltransferase isoform X4

      Conserved Domains (2) summary
      COG0500
      Location:18 → 149
      SmtA; SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only]
      cl17173
      Location:1 → 290
      AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060942.1 Alternate T2T-CHM13v2.0

      Range
      13888989..13926829
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054319300.1 → XP_054175275.1  mRNA cap guanine-N7 methyltransferase isoform X1

    2. XM_054319301.1 → XP_054175276.1  mRNA cap guanine-N7 methyltransferase isoform X1

    3. XM_054319305.1 → XP_054175280.1  mRNA cap guanine-N7 methyltransferase isoform X4

    4. XM_054319304.1 → XP_054175279.1  mRNA cap guanine-N7 methyltransferase isoform X3

      UniProtKB/Swiss-Prot
      B0YJ90, D3DUJ5, O43148, O94996, Q9UIJ9

    5. XM_054319303.1 → XP_054175278.1  mRNA cap guanine-N7 methyltransferase isoform X2

    6. XM_054319302.1 → XP_054175277.1  mRNA cap guanine-N7 methyltransferase isoform X1

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O43148.1 GenPept UniProtKB/Swiss-Prot:O43148

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