LAG1 sphingosine N-acyltransferase LAG1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: LAG1
Official Full Name: sphingosine N-acyltransferase LAG1
Primary source: SGD:S000000995
Locus tag: YHL003C
See related: AllianceGenome:SGD:S000000995; FungiDB:YHL003C; VEuPathDB:YHL003C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables sphingosine N-acyltransferase activity. Involved in ceramide biosynthetic process. Located in endoplasmic reticulum. Part of acyl-CoA ceramide synthase complex. Used to study fatty liver disease; hepatocellular carcinoma; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in autosomal recessive congenital ichthyosis 9 and progressive myoclonus epilepsy 8. Orthologous to several human genes including CERS2 (ceramide synthase 2); CERS5 (ceramide synthase 5); and CERS6 (ceramide synthase 6). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See LAG1 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (100648..101883, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

that the different specificities of the two ceramide synthases, and the specific effect of Lag1 on asymmetrical inheritance, may explain why Deltalag1 cells have an increased lifespan while Deltalac1 cells do not
Title: Yeast ceramide synthases, Lag1 and Lac1, have distinct substrate specificity.
Lac1 and Lag1 are direct targets for CK2 and that phosphorylation at conserved positions within the C-terminal cytoplasmic domain of each protein is required for optimal CerS activity
Title: Regulation of ceramide synthase by casein kinase 2-dependent phosphorylation in Saccharomyces cerevisiae.
Data demonstrate that the pleiotropic drug resistance regulatory pathway regulates the transcription of multiple genes encoding steps in sphingolipid biosynthesis, including LAC1, but not LAG1.
Title: Differential regulation of ceramide synthase components LAC1 and LAG1 in Saccharomyces cerevisiae.
the topology of the Lag1p and Lac1p subunits of ceramide synthase was investigated
Title: Transmembrane topology of ceramide synthase in yeast.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables sphingosine N-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables sphingosine N-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables sphingosine N-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables sphingosine N-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in ceramide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ceramide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ceramide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in ceramide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in sphingolipid metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of acyl-CoA ceramide synthase complex	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sphingosine N-acyltransferase LAG1

NP_011860.1

EC 2.3.1.297

Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

100648..101883 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179083.1 → NP_011860.1 TPA: sphingosine N-acyltransferase LAG1 [Saccharomyces cerevisiae S288C]

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZSP9, D3DKR1, P38703, Q6LD26

UniProtKB/TrEMBL

B3LSB5, B5VJS5, C7GX97, C8ZA62, N1P512

Conserved Domains (1) summary

COG5058
Location:8 → 411

LAG1; Protein transporter of the TRAM (translocating chain-associating membrane) superfamily, longevity assurance factor [Intracellular trafficking and secretion]