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    MED8 mediator complex subunit 8 [ Homo sapiens (human) ]

    Gene ID: 112950, updated on 2-Nov-2024

    Summary

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    Official Symbol
    MED8provided by HGNC
    Official Full Name
    mediator complex subunit 8provided by HGNC
    Primary source
    HGNC:HGNC:19971
    See related
    Ensembl:ENSG00000159479 MIM:607956; AllianceGenome:HGNC:19971
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    ARC32
    Summary
    This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
    Expression
    Ubiquitous expression in thyroid (RPKM 12.7), placenta (RPKM 11.6) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See MED8 in Genome Data Viewer
    Location:
    1p34.2
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (43383917..43389800, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (43254467..43260350, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (43849588..43855471, complement)

    Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 781 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:43832389-43833123 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:43833124-43833858 Neighboring gene uncharacterized LOC112268225 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 917 Neighboring gene ReSE screen-validated silencer GRCh37_chr1:43834951-43835136 Neighboring gene microRNA 6734 Neighboring gene ELOVL fatty acid elongase 1 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr1:43838300-43839499 Neighboring gene ReSE screen-validated silencer GRCh37_chr1:43844005-43844173 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 918 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 783 Neighboring gene MED8 antisense RNA 1 Neighboring gene SZT2 subunit of KICSTOR complex Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:43916604-43917491 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:43917492-43918378 Neighboring gene SZT2 antisense RNA 1 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 784 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 785 Neighboring gene microRNA 6735 Neighboring gene HYI antisense RNA 1 Neighboring gene hydroxypyruvate isomerase (putative)

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Nonsense mediated decay resistant mutations are a source of expressed mutant proteins in colon cancer cell lines with microsatellite instability. Williams DS, et al. PLoS One, 2010 Dec 31. PMID 21209843, Free PMC Article
    2. Interaction of Bunyamwera Orthobunyavirus NSs protein with mediator protein MED8: a mechanism for inhibiting the interferon response. Léonard VH, et al. J Virol, 2006 Oct. PMID 16973571, Free PMC Article
    3. The N-terminus of Bunyamwera orthobunyavirus NSs protein is essential for interferon antagonism. van Knippenberg I, et al. J Gen Virol, 2010 Aug. PMID 20427562, Free PMC Article
    4. Screening and identification of host factors interacting with UL14 of herpes simplex virus 1. Wu F, et al. Med Microbiol Immunol, 2011 Aug. PMID 21512757
    5. MED14 tethers mediator to the N-terminal domain of peroxisome proliferator-activated receptor gamma and is required for full transcriptional activity and adipogenesis. Grøntved L, et al. Mol Cell Biol, 2010 May. PMID 20194623, Free PMC Article

    See all (68) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. ability to inhibit both host transcription and the interferon response is linked to interaction of NSs with the MED8 component of Mediator, a protein complex necessary for mRNA production
      Title: Interaction of Bunyamwera Orthobunyavirus NSs protein with mediator protein MED8: a mechanism for inhibiting the interferon response.
    2. mammalian mediator subunit mMED8 is an elongin BC-interacting protein that can assemble with Cul2 and Rbx1 reconstitute a ubiquitin ligase (Ubc5; E2 ubiquitin-conjugating enzyme Ubc5)
      Title: Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    HIV-1 interactions

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    Replication interactions

    Interaction Pubs
    Knockdown of mediator complex subunit 8 (MED8) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat Knockdown of MED8 by siRNAs inhibits HIV LTR-beta-gal activation in the Tat transactivation assay PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Potential readthrough

    Included gene: ELOVL1

    Homology

    Clone Names

    • MGC17544, MGC19641

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription coregulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription elongation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    part_of core mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of core mediator complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of ubiquitin ligase complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    mediator of RNA polymerase II transcription subunit 8
    Names
    activator-recruited cofactor 32 kDa component
    mediator of RNA polymerase II transcription subunit MED8

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001001653.3 → NP_001001653.1  mediator of RNA polymerase II transcription subunit 8 isoform 1

      See identical proteins and their annotated locations for NP_001001653.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) contains multiple differences in the UTRs and coding region, compared to variant 3. It initiates translation at a downstream in-frame start codon. The encoded isoform (1) has shorter N- and C-termini, compared to isoform 3.
      Source sequence(s)
      AL139289, BC010019, BF196855, BG620658, CB162354
      Consensus CDS
      CCDS60108.1
      UniProtKB/Swiss-Prot
      Q96G25
      Related
      ENSP00000361533.4, ENST00000372455.4
      Conserved Domains (1) summary
      pfam10232
      Location:1 → 176
      Med8; Mediator of RNA polymerase II transcription complex subunit 8

    2. NM_052877.5 → NP_443109.2  mediator of RNA polymerase II transcription subunit 8 isoform 3

      See identical proteins and their annotated locations for NP_443109.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) represents the shortest transcript and encodes the longest isoform (3).
      Source sequence(s)
      BC010543, BG620658, BI488866
      Consensus CDS
      CCDS486.2
      UniProtKB/Swiss-Prot
      Q96G25
      Related
      ENSP00000290663.6, ENST00000290663.10
      Conserved Domains (1) summary
      pfam10232
      Location:1 → 265
      Med8; Mediator of RNA polymerase II transcription complex subunit 8

    3. NM_201542.5 → NP_963836.2  mediator of RNA polymerase II transcription subunit 8 isoform 4

      See identical proteins and their annotated locations for NP_963836.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (5) uses an alternate exon structure in the 3' coding region and differs in the 3' UTR, compared to variant 3. The resulting isoform (4) has a shorter C-terminus, compared to isoform 3.
      Source sequence(s)
      AF521562, AL139289, BC010019, BF196855, BG620658, CB162354
      Consensus CDS
      CCDS487.2
      UniProtKB/Swiss-Prot
      A9IZ91, A9IZ92, Q5JUY8, Q96FQ4, Q96G25
      Related
      ENSP00000361535.4, ENST00000372457.9
      Conserved Domains (1) summary
      pfam10232
      Location:1 → 265
      Med8; Mediator of RNA polymerase II transcription complex subunit 8

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

      Range
      43383917..43389800 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060925.1 Alternate T2T-CHM13v2.0

      Range
      43254467..43260350 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q96G25.2 GenPept UniProtKB/Swiss-Prot:Q96G25

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