pot1 shelterin complex subunit Pot1 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: pot1
Official Full Name: shelterin complex subunit Pot1
Primary source: PomBase:SPAC26H5.06
Locus tag: SPOM_SPAC26H5.06
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
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Genomic context

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See pot1 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 3

Sequence:: Chromosome: I; NC_003424.3 (4129224..4132350)

Chromosome I - NC_003424.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003424.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pot1 promotes telomere DNA replication via the Stn1-Ten1 complex in fission yeast.
Title: Pot1 promotes telomere DNA replication via the Stn1-Ten1 complex in fission yeast.
Exploring Genetic Interactions with Telomere Protection Gene pot1 in Fission Yeast.
Title: Exploring Genetic Interactions with Telomere Protection Gene pot1 in Fission Yeast.
The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.
Title: The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.
Study shows that The C-terminal domain of the DNA-binding domain, Pot1pC, exhibits non-specific ssDNA recognition and discriminates against RNA by utilizing non-compensatory binding modes that feature significant rearrangement of the binding interface.
Title: Discrimination against RNA Backbones by a ssDNA Binding Protein.
Thus, our results suggest that pik1(+) is required for the maintenance of circular chromosomes.
Title: Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1.
The conservation between fission yeast Tpz1-Pot1 and human TPP1-POT1 interactions resulted in mapping a human melanoma-associated POT1 mutation (A532P) to the TPP1-POT1 interface.
Title: Dissecting Fission Yeast Shelterin Interactions via MICro-MS Links Disruption of Shelterin Bridge to Tumorigenesis.
Long G2 phase of pot1-rqh1 double mutant may allow time for the accumulation of recombination intermediates which disturb chromosome segregation and make cells sensitive to thiabendazole.
Title: Long G2 accumulates recombination intermediates and disturbs chromosome segregation at dysfunction telomere in Schizosaccharomyces pombe.
The Pot1 DNA-binding domain is capable of binding DNA in at least two structurally distinct binding modes.
Title: The telomeric protein Pot1 from Schizosaccharomyces pombe binds ssDNA in two modes with differing 3' end availability.
Pot1 promiscuity is implemented through binding modes that thermodynamically compensate for base-substitutions through alternate stacking interactions and hydrogen-bonding networks.
Title: Nonspecific recognition is achieved in Pot1pC through the use of multiple binding modes.
Results indicate an unanticipated ssDNA-binding mode in which two SpPot1 molecules bind an oligonucleotide containing two telomeric repeats.
Title: DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables G-rich strand telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-rich strand telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded telomeric DNA binding	EXP Inferred from Experiment more info	PubMed
enables single-stranded telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables telomerase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in regulation of telomere maintenance via telomerase	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere capping	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere capping	IDA Inferred from Direct Assay more info	PubMed
involved_in telomere capping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in chromosome, telomeric repeat region	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	HDA more info	PubMed
part_of nuclear telomere cap complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	HDA more info	PubMed
part_of shelterin complex	IC Inferred by Curator more info	PubMed
part_of telomere cap complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: shelterin complex subunit Pot1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

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Reference assembly

Genomic

NC_003424.3 Reference assembly

Range

4129224..4132350

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001019882.3 → NP_594453.1 shelterin complex subunit Pot1 [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_594453.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

O13988

Conserved Domains (2) summary

smart00976
Location:27 → 171

Telo_bind; Telomeric single stranded DNA binding POT1/CDC13

pfam16686
Location:203 → 336

POT1PC; ssDNA-binding domain of telomere protection protein