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    dnaA chromosomal replication initiator protein DnaA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948217, updated on 19-Sep-2024

    Summary

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    Official Symbol
    dnaA
    Official Full Name
    chromosomal replication initiator protein DnaA
    Primary source
    ECOCYC:EG10235
    Locus tag
    b3702
    See related
    ASAP:ABE-0012103
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3694
    Summary
    DnaA-ATP is the active form; DnaA-ADP is inactive; switching betwee the two forms modulates initiation activity. [More information is available at EcoGene: EG10235].
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    Genomic context

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    Sequence:
    NC_000913.3 (3882326..3883729, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene recombination mediator protein RecF Neighboring gene beta sliding clamp Neighboring gene protein YsdD Neighboring gene 50S ribosomal subunit protein L34

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Replicatively active complexes of DnaA protein and the Escherichia coli chromosomal origin observed in the electron microscope. Crooke E, et al. J Mol Biol, 1993 Sep 5. PMID 8377183
    2. Molecular mechanism of DNA replication-coupled inactivation of the initiator protein in Escherichia coli: interaction of DnaA with the sliding clamp-loaded DNA and the sliding clamp-Hda complex. Su'etsugu M, et al. Genes Cells, 2004 Jun. PMID 15189445
    3. The DNA binding domain of the initiator protein DnaA. Roth A, et al. EMBO J, 1995 May 1. PMID 7744016, Free PMC Article
    4. Transcriptional activation of the origin of coliphage lambda DNA replication is regulated by the host DnaA initiator function. Wegrzyn G, et al. Gene, 1995 Feb 27. PMID 7867947
    5. Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box. Pérez-Roger I, et al. J Bacteriol, 1995 Apr. PMID 7896719, Free PMC Article

    See all (323) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mapping the Escherichia coli DnaA-binding landscape reveals a preference for binding pairs of closely spaced DNA sites.
      Title: Mapping the Escherichia coli DnaA-binding landscape reveals a preference for binding pairs of closely spaced DNA sites.
    2. Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein.
      Title: Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein.
    3. Concerted actions of DnaA complexes with DNA-unwinding sequences within and flanking replication origin oriC promote DnaB helicase loading.
      Title: Concerted actions of DnaA complexes with DNA-unwinding sequences within and flanking replication origin oriC promote DnaB helicase loading.
    4. Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.
      Title: Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.
    5. Topoisomerase I Essentiality, DnaA-Independent Chromosomal Replication, and Transcription-Replication Conflict in Escherichia coli.
      Title: Topoisomerase I Essentiality, DnaA-Independent Chromosomal Replication, and Transcription-Replication Conflict in Escherichia coli.
    6. Inhibition of Escherichia coli chromosome replication by rifampicin treatment or during the stringent response is overcome by de novo DnaA protein synthesis.
      Title: Inhibition of Escherichia coli chromosome replication by rifampicin treatment or during the stringent response is overcome by de novo DnaA protein synthesis.
    7. H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division.
      Title: H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division.
    8. A model for DnaA-reactivating sequences-DnaA complex dynamics during ADP dissociation; this provides novel insight into the regulatory mechanisms of DnaA and the interaction modes of AAA+ domains.
      Title: A novel mode of DnaA-DnaA interaction promotes ADP dissociation for reactivation of replication initiation activity.
    9. A nucleotide-dependent oligomerization of the Escherichia coli replication initiator DnaA requires residue His136 for remodeling of the chromosomal origin.
      Title: A nucleotide-dependent oligomerization of the Escherichia coli replication initiator DnaA requires residue His136 for remodeling of the chromosomal origin.
    10. The findings indicate both DnaA-ATP and DnaA-ADP can perform most of the mechanical functions needed for origin activation, and suggest that a key reason for ATP-regulation of DnaA is to control replication initiation frequency.
      Title: Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome replication.
    Submit: New GeneRIF Correction See all GeneRIFs (57)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA replication origin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA replication origin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA replication origin binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA replication initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication initiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA unwinding involved in DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in negative regulation of DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of DNA-templated DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA-templated DNA replication initiation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DnaA-DiaA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of DnaA-Dps complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of DnaA-HU complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of DnaA-L2 complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of DnaA-oriC complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasmic side of plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of replication inhibiting complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    chromosomal replication initiator protein DnaA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3882326..3883729 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418157.1 chromosomal replication initiator protein DnaA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418157.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1MPE7
      Conserved Domains (1) summary
      TIGR00362
      Location:6 → 465
      DnaA; chromosomal replication initiator protein DnaA
    Nucleotide Protein
    Heading Accession and Version

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