Ago3 argonaute RISC catalytic subunit 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ago3provided by MGI
Official Full Name: argonaute RISC catalytic subunit 3provided by MGI
Primary source: MGI:MGI:2446634
See related: Ensembl:ENSMUSG00000028842 AllianceGenome:MGI:2446634
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Eif2c3; C130014L07Rik
Summary: Enables RNA binding activity. Involved in positive regulation of gene expression and positive regulation of non-canonical NF-kappaB signal transduction. Located in condensed nuclear chromosome. Part of RISC complex. Is expressed in several structures, including central nervous system; early embryo; lung mesenchyme; sensory organ; and tooth. Orthologous to human AGO3 (argonaute RISC catalytic component 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in cerebellum adult (RPKM 2.8), frontal lobe adult (RPKM 2.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 D2.2; 4 60.39 cM

Exon count:: 24

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (126220797..126323360, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (126326994..126431670, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity.
Title: Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity.
Results from the liver show that, siRNA targets 3'UTR and the coding sequence (CDs) of endogenous genes in the presence Ago2 but in its absence, only 3'UTR-targeted siRNA-mediated knockdown are active with the help of Ago1 and Ago3.
Title: The roles of individual mammalian argonautes in RNA interference in vivo.
enhanced flu susceptibility of Ago1/3 double-knockout mice arises from an intrinsic impairment in the ability of lung cells to tolerate flu-elicited inflammation.
Title: Enhanced susceptibility of Ago1/3 double-null mice to influenza A virus infection.
EIF2C2-4 and PIWIL4 appear increased in advanced tumors with distant metastasis, suggesting they may promote tumor invasion
Title: Argonaute proteins: potential biomarkers for human colon cancer.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Eif2c3 (e-PCR), detects polymorphism
- UniSTS:471828

BB553547 (e-PCR)
- UniSTS:207675

BB192386 (e-PCR)
- UniSTS:207677

BB466098 (e-PCR)
- UniSTS:207678

Eif2c3 (e-PCR), detects polymorphism
- UniSTS:479103

RH124337 (e-PCR)
- UniSTS:161159

AW048688 (e-PCR)
- UniSTS:165354

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	ISO Inferred from Sequence Orthology more info
enables RNA endonuclease activity	ISS Inferred from Sequence or Structural Similarity more info
enables double-stranded RNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	ISO Inferred from Sequence Orthology more info
enables endoribonuclease activity, cleaving miRNA-paired mRNA	ISO Inferred from Sequence Orthology more info
enables endoribonuclease activity, cleaving miRNA-paired mRNA	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IEA Inferred from Electronic Annotation more info
enables miRNA binding	ISO Inferred from Sequence Orthology more info
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IEA Inferred from Electronic Annotation more info
enables single-stranded RNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IEA Inferred from Electronic Annotation more info
involved_in RISC complex assembly	ISO Inferred from Sequence Orthology more info
involved_in RNA secondary structure unwinding	IEA Inferred from Electronic Annotation more info
involved_in RNA secondary structure unwinding	ISO Inferred from Sequence Orthology more info
involved_in mRNA catabolic process	ISO Inferred from Sequence Orthology more info
involved_in mRNA catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by inhibition of translation	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by inhibition of translation	ISS Inferred from Sequence or Structural Similarity more info
involved_in miRNA-mediated gene silencing by mRNA destabilization	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of non-canonical NF-kappaB signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pre-miRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in pre-miRNA processing	IEA Inferred from Electronic Annotation more info
involved_in pre-miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in regulation of stem cell proliferation	IEA Inferred from Electronic Annotation more info
involved_in regulation of stem cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in P-body	IEA Inferred from Electronic Annotation more info
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC complex	ISO Inferred from Sequence Orthology more info
part_of RISC-loading complex	IEA Inferred from Electronic Annotation more info
part_of RISC-loading complex	ISO Inferred from Sequence Orthology more info
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic ribonucleoprotein granule	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: protein argonaute-3

Names: Piwi/Argonaute family protein meIF2C3; argonaute 3; argonaute RISC catalytic component 3; argonaute3; eIF-2C 3; eIF2C 3; eukaryotic translation initiation factor 2C, 3; mAgo3

NP_700451.2

EC 3.1.26.n2

XP_006502980.1

EC 3.1.26.n2

XP_011238780.1

EC 3.1.26.n2

XP_017175589.1

EC 3.1.26.n2

XP_030109249.1

EC 3.1.26.n2

XP_030109250.1

EC 3.1.26.n2

XP_030109251.1

EC 3.1.26.n2

XP_036019842.1

EC 3.1.26.n2

XP_036019844.1

EC 3.1.26.n2

XP_036019845.1

EC 3.1.26.n2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_153402.2 → NP_700451.2 protein argonaute-3

See identical proteins and their annotated locations for NP_700451.2

Status: VALIDATED

Source sequence(s)

AK083652, AL606976, BY235425

Consensus CDS

CCDS18652.1

UniProtKB/Swiss-Prot

Q3TBP7, Q8CJF9

UniProtKB/TrEMBL

Q7M738

Related

ENSMUSP00000066633.7, ENSMUST00000069097.13

Conserved Domains (5) summary

PLN03202
Location:20 → 859

PLN03202; protein argonaute; Provisional

cd02846
Location:228 → 348

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:393 → 818

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:178 → 227

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:20 → 167

ArgoN; N-terminal domain of argonaute

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

126220797..126323360 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006502917.5 → XP_006502980.1 protein argonaute-3 isoform X2

UniProtKB/TrEMBL

Q7M738

Conserved Domains (4) summary

cd02846
Location:228 → 348

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:393 → 818

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:177 → 227

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:73 → 167

ArgoN; N-terminal domain of argonaute
XM_011240478.4 → XP_011238780.1 protein argonaute-3 isoform X1

UniProtKB/TrEMBL

Q7M738

Conserved Domains (4) summary

cd02846
Location:228 → 348

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:393 → 818

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:177 → 227

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:73 → 167

ArgoN; N-terminal domain of argonaute
XM_036163952.1 → XP_036019845.1 protein argonaute-3 isoform X3

Conserved Domains (2) summary

cd02846
Location:1 → 114

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:159 → 584

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
XM_030253390.2 → XP_030109250.1 protein argonaute-3 isoform X4

Conserved Domains (2) summary

cd04657
Location:118 → 543

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam02170
Location:1 → 90

PAZ; PAZ domain
XM_030253389.2 → XP_030109249.1 protein argonaute-3 isoform X4

Conserved Domains (2) summary

cd04657
Location:118 → 543

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam02170
Location:1 → 90

PAZ; PAZ domain
XM_036163949.1 → XP_036019842.1 protein argonaute-3 isoform X3

Conserved Domains (2) summary

cd02846
Location:1 → 114

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:159 → 584

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
XM_030253391.2 → XP_030109251.1 protein argonaute-3 isoform X4

Conserved Domains (2) summary

cd04657
Location:118 → 543

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam02170
Location:1 → 90

PAZ; PAZ domain
XM_036163951.1 → XP_036019844.1 protein argonaute-3 isoform X3

Conserved Domains (2) summary

cd02846
Location:1 → 114

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:159 → 584

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
XM_017320100.2 → XP_017175589.1 protein argonaute-3 isoform X4

Conserved Domains (2) summary

cd04657
Location:118 → 543

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam02170
Location:1 → 90

PAZ; PAZ domain