let-60 Ras protein let-60 [Caenorhabditis elegans] - Gene

Summary

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Gene symbol: let-60
Gene description: Ras protein let-60
Primary source: WormBase:WBGene00002335
Locus tag: CELE_ZK792.6
See related: AllianceGenome:WB:WBGene00002335
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables GTP binding activity; phospholipase binding activity; and protein kinase binding activity. Involved in several processes, including learning or memory; positive regulation of receptor localization to synapse; and positive regulation of vulval development. Located in cell cortex. Is expressed in several structures, including germ line; gonad; head neurons; pharyngeal muscle cell; and vulval cell. Used to study RASopathy and cancer. Human ortholog(s) of this gene implicated in several diseases, including Noonan syndrome (multiple); carcinoma (multiple); cardiofaciocutaneous syndrome (multiple); hematologic cancer (multiple); and high grade glioma (multiple). Orthologous to several human genes including HRAS (HRas proto-oncogene, GTPase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See let-60 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 4

Sequence:: Chromosome: IV; NC_003282.8 (11688227..11691088, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ROS regulation of RAS and vulva development in Caenorhabditis elegans.
Title: ROS regulation of RAS and vulva development in Caenorhabditis elegans.
We conclude that RAP-1 is a positive regulator that promotes Ras-dependent inductive fate decisions. We hypothesize that PXF-1 activation of RAP-1 serves as a minor parallel input into the major LET-60/Ras signal through LIN-45/Raf
Title: Ras-Dependent Cell Fate Decisions Are Reinforced by the RAP-1 Small GTPase in Caenorhabditiselegans.
Expression of activated LET-60/Ras in the neighboring duct cell, but not in the G1 or G2 cells, is sufficient to rescue sos-1 delamination defects, revealing that Ras acts non-cell-autonomously to permit G1 delamination.
Title: A non-cell-autonomous role for Ras signaling in C. elegans neuroblast delamination.
BET-1 as a Sumo-dependent attenuator of LET-60-mediated signalling and our characterisation suggests that BET-1 can be sumoylated.
Title: An RNAi-based dimorphic genetic screen identified the double bromodomain protein BET-1 as a sumo-dependent attenuator of RAS-mediated signalling.
Canal cell asymmetry and let-60/Ras signaling influence which of two equivalent precursors will attach to the canal cell.
Title: Notch and Ras promote sequential steps of excretory tube development in C. elegans.
LET-60 (Ras) is identified as a critical RGEF-1b substrate-effector in sensory neurons.
Title: A RasGRP, C. elegans RGEF-1b, couples external stimuli to behavior by activating LET-60 (Ras) in sensory neurons.
Goa-1 Goalpha and let-60 Ras act in parallel to gpc-1 in olfactory pathway.
Title: GPC-1, a G protein gamma-subunit, regulates olfactory adaptation in Caenorhabditis elegans.
LET-60 Ras GTPase might act with FLI-1 to control germ line morphogenesis
Title: FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in C. elegans.
Data show that an activated Ras mutation, let-60, weakly suppresses constitutive dauer diapause in daf-2 and age-1 (PI3K) mutants.
Title: LET-60 RAS modulates effects of insulin/IGF-1 signaling on development and aging in Caenorhabditis elegans.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ZK792.6 (e-PCR)
- UniSTS:319849

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables GDP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables GTPase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phospholipase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in associative learning	IGI Inferred from Genetic Interaction more info	PubMed
involved_in epidermal growth factor receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in epidermal growth factor receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in muscle organ development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in muscle organ development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nematode larval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nematode larval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of receptor localization to synapse	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of vulval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of vulval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell projection organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell projection organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of vulval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in reproductive process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in short-term memory	IGI Inferred from Genetic Interaction more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in vulval cell fate specification	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vulval cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Ras protein let-60

NP_502213.3

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

11688227..11691088 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_069812.5 → NP_502213.3 Ras protein let-60 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_502213.3

Status: REVIEWED

UniProtKB/Swiss-Prot

P22981

Conserved Domains (2) summary

cd04138
Location:3 → 164

H_N_K_Ras_like; Ras GTPase family containing H-Ras,N-Ras and K-Ras4A/4B

smart00010
Location:16 → 166

small_GTPase; Small GTPase of the Ras superfamily; ill-defined subfamily