FUS2 Fus2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: FUS2
Official Full Name: Fus2p
Primary source: SGD:S000004845
Locus tag: YMR232W
See related: AllianceGenome:SGD:S000004845; FungiDB:YMR232W; VEuPathDB:YMR232W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Predicted to enable guanyl-nucleotide exchange factor activity. Involved in cytogamy; karyogamy involved in conjugation with cellular fusion; and regulation of termination of mating projection growth. Located in cytoplasm; mating projection tip; and nucleus. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See FUS2 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (736926..738959)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Fus2p localizes Cdc42p to the flat zone of cell fusion to promote cell wall degradation during cell fusion.
Title: Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion.
Cdc42p acts at a late stage in cell fusion in concert with a key cell fusion regulator, Fus2p, which contains a Dbl-homology domain. We find that Fus2p binds specifically with activated Cdc42p, and binding is blocked by the cdc42-138 mutation.
Title: Cdc42p and Fus2p act together late in yeast cell fusion.
potential premature activation of Fus2p in mitosis is prevented by cell cycle-dependent phosphorylation that overrides the mating pheromone-induced phosphorylation that drives nuclear export.
Title: A mechanism for the coordination of proliferation and differentiation by spatial regulation of Fus2p in budding yeast.
Fus2p and Sec4p, another Myo2p cargo associated with exocytotic vesicles, reside predominantly on different cellular organelles.
Title: The class V myosin Myo2p is required for Fus2p transport and actin polarization during the yeast mating response.
Results indicate a novel mechanism by which pheromone-induced proteins, including Fus2, are regulated during the transition from mitosis to conjugation.
Title: Antagonistic regulation of Fus2p nuclear localization by pheromone signaling and the cell cycle.
Fus2p dots are transported along actin cables, possibly in association with vesicles.
Title: Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytogamy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cytogamy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in karyogamy involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actomyosin contractile ring contraction	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of division septum assembly	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cell tip	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Fus2p

NP_013959.1

Cell fusion regulator; cytoplasmic protein localized to shmoo tip; required for alignment of parental nuclei before nuclear fusion during mating; contains a Dbl-homology domain; binds specifically with activated Cdc42p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

736926..738959

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182739.1 → NP_013959.1 TPA: Fus2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013959.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W058, Q05023, Q05670

UniProtKB/TrEMBL

B3LMC1, C7GRB8, C8ZF97, N1P0K3

Conserved Domains (1) summary

cl02571
Location:114 → 301

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.