| Proteomics, modeling, and fluorescence assays delineate cytochrome b5 residues involved in binding and stimulation of cytochrome P450 17A1 17,20-lyase. | Proteomics, modeling, and fluorescence assays delineate cytochrome b(5) residues involved in binding and stimulation of cytochrome P450 17A1 17,20-lyase.
Tateishi Y, Webb SN, Li B, Liu L, Lindsey Rose K, Leser M, Patel P, Guengerich FP., Free PMC Article | 04/5/2024 |
| The N-terminal intrinsically disordered region of Ncb5or docks with the cytochrome b5 core to form a helical motif that is of ancient origin. | The N-terminal intrinsically disordered region of Ncb5or docks with the cytochrome b(5) core to form a helical motif that is of ancient origin.
Benson DR, Deng B, Kashipathy MM, Lovell S, Battaile KP, Cooper A, Gao P, Fenton AW, Zhu H., | 03/8/2024 |
| The association between CYB5A gene rs1790834 polymorphism and clinical improvement after 6 months of leflunomide treatment in women with rheumatoid arthritis. | The association between CYB5A gene rs1790834 polymorphism and clinical improvement after 6 months of leflunomide treatment in women with rheumatoid arthritis.
Machaj F, Rosik J, Szostak B, Malinowski D, Safranow K, Olędzka G, Wiechec E, Pawlik A. | 08/10/2023 |
| Regulating Effect of Cytochrome b5 Overexpression on Human Breast Cancer Cells. | Regulating Effect of Cytochrome b(5) Overexpression on Human Breast Cancer Cells.
Tong XY, Yang XZ, Gao SQ, Wang XJ, Wen GB, Lin YW., Free PMC Article | 07/30/2022 |
| An alternative CYB5A transcript is expressed in aneuploid ALL and enriched in relapse. | An alternative CYB5A transcript is expressed in aneuploid ALL and enriched in relapse.
Bartsch L, Schroeder MP, Hänzelmann S, Bastian L, Lázaro-Navarro J, Schlee C, Tanchez JO, Schulze V, Isaakidis K, Rieger MA, Gökbuget N, Eckert C, Serve H, Horstmann M, Schrappe M, Brüggemann M, Baldus CD, Neumann M., Free PMC Article | 07/9/2022 |
| Functional variants in cytochrome b5 type A (CYB5A) are enriched in Southwest American Indian individuals and associate with obesity. | Functional variants in cytochrome b5 type A (CYB5A) are enriched in Southwest American Indian individuals and associate with obesity.
Day SE, Traurig M, Kumar P, Piaggi P, Koroglu C, Kobes S, Hanson RL, Bogardus C, Baier LJ., Free PMC Article | 04/23/2022 |
| Lack of association between CYB5A gene rs1790834 polymorphism and the response to leflunomide in women with rheumatoid arthritis. | Lack of association between CYB5A gene rs1790834 polymorphism and the response to leflunomide in women with rheumatoid arthritis.
Łączna M, Malinowski D, Paradowska-Gorycka A, Safranow K, Dziedziejko V, Pawlik A., Free PMC Article | 02/5/2022 |
| Isolated 17, 20 Lyase Deficiency Secondary to a Novel CYB5A Variant: Comparison of Steroid Metabolomic Findings with Published Cases Provides Diagnostic Guidelines and Greater Insight into Its Biological Role. | Isolated 17, 20 Lyase Deficiency Secondary to a Novel CYB5A Variant: Comparison of Steroid Metabolomic Findings with Published Cases Provides Diagnostic Guidelines and Greater Insight into Its Biological Role.
Shaunak M, Taylor NF, Hunt D, Davies JH. | 11/13/2021 |
| A homodimer model can resolve the conundrum as to how cytochrome P450 oxidoreductase and cytochrome b5 compete for the same binding site on cytochrome P450c17. (Review) | A Homodimer Model Can Resolve the Conundrum as to How Cytochrome P450 Oxidoreductase and Cytochrome b5 Compete for the Same Binding Site on Cytochrome P450c17.
Holien JK, Parker MW, Conley AJ, Corbin CJ, Rodgers RJ, Martin LL. | 11/4/2017 |
| Data suggest CYP17A1, with electron donor NADPH-P450 reductase, is inherently distributive enzyme but that some processivity is present; some 17alpha-hydroxy pregnenolone formed does not dissociate from CYP17A1 before conversion to dehydroepiandrosterone; CYB5A does not enhance reaction by decreasing k(off) of ligands of CYP17A1. (CYP17A1 = cytochrome P450, family 17, subfamily A, polypeptide 1; CYB5A = cytochrome b5) | Kinetic processivity of the two-step oxidations of progesterone and pregnenolone to androgens by human cytochrome P450 17A1.
Gonzalez E, Guengerich FP., Free PMC Article | 08/26/2017 |
| Data suggest that cytochrome b5 (CYB5) and cytochrome b5 reductase 3 (CYB5R3) can reduce human cytoglobin (CYGB) and zebrafish cytoglobins at rates up to 250-fold higher than those reported for the known physiological substrates, hemoglobin and myoglobin; the three proteins (CYB5+CYB5R3+CYGB) appear to constitute a metabolon involved in generation of nitric oxide. | Efficient Reduction of Vertebrate Cytoglobins by the Cytochrome b(5)/Cytochrome b(5) Reductase/NADH System.
Amdahl MB, Sparacino-Watkins CE, Corti P, Gladwin MT, Tejero J., Free PMC Article | 08/12/2017 |
| Thus, although Mn-b5 binds to CYP17A1, it is unable to enhance the lyase reaction, strongly suggesting that cyt b5 has a redox effector role in enhancement of the CYP17A1 mediated lyase reaction necessary for androgen synthesis. | Evidence that cytochrome b5 acts as a redox donor in CYP17A1 mediated androgen synthesis.
Duggal R, Liu Y, Gregory MC, Denisov IG, Kincaid JR, Sligar SG., Free PMC Article | 06/24/2017 |
| This study constructed a model of the membrane-bound full-length human P450 1A2-cyt b5 complex. The model was assembled from several parts using a multiscale modeling approach covering all-atom and coarse-grained molecular dynamics (MD). | Membrane-Anchored Cytochrome P450 1A2-Cytochrome b5 Complex Features an X-Shaped Contact between Antiparallel Transmembrane Helices.
Jeřábek P, Florián J, Martínek V. | 12/17/2016 |
| We conclude that cytochrome b5 can influence the electronic conductivity of cytochrome P450c17 via allosteric, protein-protein interactions. | Mechanistic Scrutiny Identifies a Kinetic Role for Cytochrome b5 Regulation of Human Cytochrome P450c17 (CYP17A1, P450 17A1).
Simonov AN, Holien JK, Yeung JC, Nguyen AD, Corbin CJ, Zheng J, Kuznetsov VL, Auchus RJ, Conley AJ, Bond AM, Parker MW, Rodgers RJ, Martin LL., Free PMC Article | 06/28/2016 |
| Variants in CYB5A and CYB5R3 should be considered in the evaluation of breast cancer risk in African American women. | Polymorphisms in the carcinogen detoxification genes CYB5A and CYB5R3 and breast cancer risk in African American women.
Blanke KL, Sacco JC, Millikan RC, Olshan AF, Luo J, Trepanier LA., Free PMC Article | 04/9/2016 |
| CYB5A is the first RA susceptibility gene involved in androgen synthesis. Our functional analysis of SNP rs1790834 indicates that it contributes to the sex bias observed in RA. | CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women.
Stark K, Straub RH, Rovenský J, Blažičková S, Eiselt G, Schmidt M., Free PMC Article | 01/16/2016 |
| both area and ratio of 3betaHSD and CYB5A double positive cells, which could represent the hybrid cells of zona fasciculate and zona reticularis, are correlated with adrenal development and could influence age-related serum androstenedione levels. | 3βHSD and CYB5A double positive adrenocortical cells during adrenal development/aging.
Nakamura Y, Fujishima F, Hui XG, Felizola SJ, Shibahara Y, Akahira J, McNamara KM, Rainey WE, Sasano H., Free PMC Article | 09/12/2015 |
| Two acidic residues, Glu-48 and Glu-49, of cytochrome b5 are essential for stimulating the 17,20-lyase activity of CYP17A1. | Catalytically relevant electrostatic interactions of cytochrome P450c17 (CYP17A1) and cytochrome b5.
Peng HM, Liu J, Forsberg SE, Tran HT, Anderson SM, Auchus RJ., Free PMC Article | 03/7/2015 |
| raises the possibility that inhibition of CYB5A-deregulated downstream pathways, such as those involving TRAF6, may favor autophagy-mediated cancer cell death in selected subgroups of patients | CYB5A and autophagy-mediated cell death in pancreatic cancer.
Giovannetti E, Giaccone G., Free PMC Article | 01/31/2015 |
| CYB5A, which has a role in stearyl-CoA-desaturase activity, and RNF10, with an unknown role in weight regulating pathways, associated with adiposity and nominally increased the risk for T2D in American Indians. | Whole exome sequencing identifies variation in CYB5A and RNF10 associated with adiposity and type 2 diabetes.
Huang K, Nair AK, Muller YL, Piaggi P, Bian L, Del Rosario M, Knowler WC, Kobes S, Hanson RL, Bogardus C, Baier LJ., Free PMC Article | 01/24/2015 |
| Cytochrome b5 seems to play the key role in the formation of lipid-radical cycles in membranes. | Role of cytochrome b5 and α-tocopherol to microsomal and mitochondrial oxidation.
Agadzhanyan ZS, Doroshchuk AD, Shiryaeva YK, Dmitriev LF. | 09/6/2014 |
| Results define CYB5A as a novel prognostic factor for PDAC that exerts its tumor-suppressor function through autophagy induction and TRAF6 modulation. | Role of CYB5A in pancreatic cancer prognosis and autophagy modulation.
Giovannetti E, Wang Q, Avan A, Funel N, Lagerweij T, Lee JH, Caretti V, van der Velde A, Boggi U, Wang Y, Vasile E, Peters GJ, Wurdinger T, Giaccone G., Free PMC Article | 03/1/2014 |
| CYP17A1/b5 interaction is stronger when the hydroxylase substrate pregnenolone is present in the CYP17A1 active site than when the lyase substrate 17alpha-hydroxypregnenolone is in the active site. | Substrate-modulated cytochrome P450 17A1 and cytochrome b5 interactions revealed by NMR.
Estrada DF, Laurence JS, Scott EE., Free PMC Article | 08/31/2013 |
| Identification of the first human CYB5A missense mutation as the cause of isolated 17,20 lyase deficiency in three individuals with 46,XY disorder of sex development. | A missense mutation in the human cytochrome b5 gene causes 46,XY disorder of sex development due to true isolated 17,20 lyase deficiency.
Idkowiak J, Randell T, Dhir V, Patel P, Shackleton CH, Taylor NF, Krone N, Arlt W., Free PMC Article | 07/7/2012 |
| Human adrenal cells that express both 3beta-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production | Human adrenal cells that express both 3β-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production.
Nakamura Y, Xing Y, Hui XG, Kurotaki Y, Ono K, Cohen T, Sasano H, Rainey WE., Free PMC Article | 06/25/2011 |