| UPF1 helicase orchestrates mutually exclusive interactions with the SMG6 endonuclease and UPF2. | UPF1 helicase orchestrates mutually exclusive interactions with the SMG6 endonuclease and UPF2.
Langer LM, Kurscheidt K, Basquin J, Bonneau F, Iermak I, Basquin C, Conti E., Free PMC Article | 06/11/2024 |
| An alternative spliced UPF2 transcript in pancreatic inflammatory myofibroblastic tumors. | An alternative spliced UPF2 transcript in pancreatic inflammatory myofibroblastic tumors.
Jiang H, Zhang Y, Hu J, Wang Z, Li G, Lu Y. | 01/4/2024 |
| Modulation of RNA-binding properties of the RNA helicase UPF1 by its activator UPF2. | Modulation of RNA-binding properties of the RNA helicase UPF1 by its activator UPF2.
Xue G, Maciej VD, Machado de Amorim A, Pak M, Jayachandran U, Chakrabarti S., Free PMC Article | 01/28/2023 |
| Structures of nonsense-mediated mRNA decay factors UPF3B and UPF3A in complex with UPF2 reveal molecular basis for competitive binding and for neurodevelopmental disorder-causing mutation. | Structures of nonsense-mediated mRNA decay factors UPF3B and UPF3A in complex with UPF2 reveal molecular basis for competitive binding and for neurodevelopmental disorder-causing mutation.
Bufton JC, Powers KT, Szeto JA, Toelzer C, Berger I, Schaffitzel C., Free PMC Article | 06/18/2022 |
| our findings indicate that impaired UPF2-dependent nonsense-mediated decay leads to neurodevelopmental dysfunction | Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response.
Johnson JL, Stoica L, Liu Y, Zhu PJ, Bhattacharya A, Buffington SA, Huq R, Eissa NT, Larsson O, Porse BT, Domingo D, Nawaz U, Carroll R, Jolly L, Scerri TS, Kim HG, Brignell A, Coleman MJ, Braden R, Kini U, Jackson V, Baxter A, Bahlo M, Scheffer IE, Amor DJ, Hildebrand MS, Bonnen PE, Beeton C, Gecz J, Morgan AT, Costa-Mattioli M., Free PMC Article | 03/28/2020 |
| UPF2 acts as an adaptor between Stau1 and UPF1, stimulates the catalytic activity of UPF1 and plays a central role in the formation of an Staufen-mediated mRNA decay-competent mRNP. | Insights into the assembly and architecture of a Staufen-mediated mRNA decay (SMD)-competent mRNP.
Gowravaram M, Schwarz J, Khilji SK, Urlaub H, Chakrabarti S., Free PMC Article | 03/14/2020 |
| we find that the interaction of UPF2 with UPF3b interferes with the assembly of the UPF2-eRF3 complex, and that UPF2 binds UPF3b more strongly than eRF3 | Human nonsense-mediated mRNA decay factor UPF2 interacts directly with eRF3 and the SURF complex.
López-Perrote A, Castaño R, Melero R, Zamarro T, Kurosawa H, Ohnishi T, Uchiyama A, Aoyagi K, Buchwald G, Kataoka N, Yamashita A, Llorca O., Free PMC Article | 07/30/2016 |
| UPF2 binds the FRB domain of SMG1, a region that regulates the related mTOR kinase. | Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD.
Melero R, Uchiyama A, Castaño R, Kataoka N, Kurosawa H, Ohno S, Yamashita A, Llorca O. | 04/4/2015 |
| This study demonstrated the quantitative regulation of Upf1 and Upf2 proteins by ubiquitin-proteasome system and SMG1. | Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells.
Zhao X, Nogawa A, Matsunaga T, Takegami T, Nakagawa H, Ishigaki Y. | 08/23/2014 |
| UPF2 MIF4G-1 and MIF4G-2 domains appear to have a crucial scaffolding role, while the MIF4G-3 domain is the key module required for triggering nonsense-mediated decay. | Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2.
Clerici M, Deniaud A, Boehm V, Gehring NH, Schaffitzel C, Cusack S., Free PMC Article | 05/10/2014 |
| Data show that upon binding to Upf2, the regulatory CH domain of Upf1 undergoes a large conformational change, causing the catalytic helicase domain to bind RNA less extensively and triggering its helicase activity. | Molecular mechanisms for the RNA-dependent ATPase activity of Upf1 and its regulation by Upf2.
Chakrabarti S, Jayachandran U, Bonneau F, Fiorini F, Basquin C, Domcke S, Le Hir H, Conti E. | 05/21/2011 |
| The authors propose that the bipartite mode of UPF2 binding to UPF1 brings the ribosome and the exon junction complex in close proximity by forming a tight complex after an initial weak encounter with either element. | Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2.
Clerici M, Mourão A, Gutsche I, Gehring NH, Hentze MW, Kulozik A, Kadlec J, Sattler M, Cusack S., Free PMC Article | 07/5/2010 |
| Observational study of gene-disease association. (HuGE Navigator) | A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease.
Grupe A, Li Y, Rowland C, Nowotny P, Hinrichs AL, Smemo S, Kauwe JS, Maxwell TJ, Cherny S, Doil L, Tacey K, van Luchene R, Myers A, Wavrant-De Vrièze F, Kaleem M, Hollingworth P, Jehu L, Foy C, Archer N, Hamilton G, Holmans P, Morris CM, Catanese J, Sninsky J, White TJ, Powell J, Hardy J, O'Donovan M, Lovestone S, Jones L, Morris JC, Thal L, Owen M, Williams J, Goate A., Free PMC Article | 12/2/2009 |
| Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) | Screening for replication of genome-wide SNP associations in sporadic ALS.
Cronin S, Tomik B, Bradley DG, Slowik A, Hardiman O., Free PMC Article | 11/19/2008 |
| UPF2 and UPF3b cooperatively stimulate both ATPase and RNA helicase activities of UPF1. | NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity.
Chamieh H, Ballut L, Bonneau F, Le Hir H. | 01/21/2010 |
| The Upf complex communicates with the exon-junction complex and triggers nonsense-mediated decay in the cytoplasm. | Communication with the exon-junction complex and activation of nonsense-mediated decay by human Upf proteins occur in the cytoplasm.
Singh G, Jakob S, Kleedehn MG, Lykke-Andersen J. | 01/21/2010 |
| UPF2-silenced HeLa cells were impaired in their ability to recognize ectopically expressed aberrant premature termination codon transcripts | hUPF2 silencing identifies physiologic substrates of mammalian nonsense-mediated mRNA decay.
Wittmann J, Hol EM, Jäck HM., Free PMC Article | 01/21/2010 |
| During nonsense-mediated mRNA decay, CBP80 interacts with Upf1 and promotes the interaction of Upf1 with Upf2 but not with Stau1. | CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells.
Hosoda N, Kim YK, Lejeune F, Maquat LE. | 01/21/2010 |
| The complex between the interacting domains of human UPF2 and UPF3b at a 1.95 A resolution. | The structural basis for the interaction between nonsense-mediated mRNA decay factors UPF2 and UPF3.
Kadlec J, Izaurralde E, Cusack S. | 01/21/2010 |