| We propose that Armi shuttles between nuage and mitochondria, feeding precursor piRNAs generated by Ago3 cleavage into the Zucchini-dependent production of Aubergine- and Piwi-bound piRNAs on the mitochondrial surface. | The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria.
Ge DT, Wang W, Tipping C, Gainetdinov I, Weng Z, Zamore PD., Free PMC Article | 10/26/2019 |
| Data show that Cbp80 knockdown not only led to the upregulation of transposons, but also to delocalization of Piwi, Aub and Ago3, key factors in the piRNA biosynthesis pathway. | Cbp80 is needed for the expression of piRNA components and piRNAs.
Rashpa R, Vazquez-Pianzola P, Colombo M, Hernandez G, Beuchle D, Berger F, Peischl S, Bruggmann R, Suter B., Free PMC Article | 10/28/2017 |
| The lack of Spn-E caused a significant drop of protein levels of key ping-pong participants, Aubergine (Aub) and AGO3 proteins of PIWI subfamily, in the germline of both males and females, but did not disrupt of their assembly in nuage granules. | RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila.
Ryazansky SS, Kotov AA, Kibanov MV, Akulenko NV, Korbut AP, Lavrov SA, Gvozdev VA, Olenina LV. | 03/18/2017 |
| We find there are large differences in evolutionary rates and gene turnover between pathways, and that paralogs of Ago2, Ago3, and Piwi/Aub show contrasting rates of evolution after duplication. | Duplication and Diversification of Dipteran Argonaute Genes, and the Evolutionary Divergence of Piwi and Aubergine.
Lewis SH, Salmela H, Obbard DJ., Free PMC Article | 03/4/2017 |
| Krimper interacts directly with Aub and Ago3 to coordinate the assembly of the ping-pong piRNA processing complex. | Aub and Ago3 Are Recruited to Nuage through Two Mechanisms to Form a Ping-Pong Complex Assembled by Krimper.
Webster A, Li S, Hur JK, Wachsmuth M, Bois JS, Perkins EM, Patel DJ, Aravin AA., Free PMC Article | 04/30/2016 |
| Krimp enforces an antisense bias on piRNA pools by binding AGO3 and blocking its access to primary piRNAs. | Krimper Enforces an Antisense Bias on piRNA Pools by Binding AGO3 in the Drosophila Germline.
Sato K, Iwasaki YW, Shibuya A, Carninci P, Tsuchizawa Y, Ishizu H, Siomi MC, Siomi H. | 04/30/2016 |
| Although Argonaute3 slicing is required to efficiently trigger phased piRNA production, an alternative, slicing-independent pathway suffices to generate Piwi-bound piRNAs that repress transcription of a subset of transposon families. | Slicing and Binding by Ago3 or Aub Trigger Piwi-Bound piRNA Production by Distinct Mechanisms.
Wang W, Han BW, Tipping C, Ge DT, Zhang Z, Weng Z, Zamore PD., Free PMC Article | 11/28/2015 |
| AGO3 Slicer activity is essential for piRNA amplification. | AGO3 Slicer activity regulates mitochondria-nuage localization of Armitage and piRNA amplification.
Huang H, Li Y, Szulwach KE, Zhang G, Jin P, Chen D., Free PMC Article | 10/4/2014 |
| PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition. | PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition.
Liu L, Qi H, Wang J, Lin H., Free PMC Article | 07/2/2011 |
| Biogenesis pathways of piRNAs loaded onto AGO3 in testes. | Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis.
Nagao A, Mituyama T, Huang H, Chen D, Siomi MC, Siomi H., Free PMC Article | 01/1/2011 |
| Studies show that Drosophila melanogaster has three PIWI proteins: PIWI, AUBERGINE (AUB), and ARGONAUTE3 (AGO3) and it has been shown that Drosophila PIWI is mostly a nuclear protein [10] and is involved in the asymmetric division of germline stem cells. | PIWI, piRNAs, and germline stem cells: what's the link?
Samji T., Free PMC Article | 01/21/2010 |
| Ago3 acts to amplify piRNA pools and to enforce on them an antisense bias, increasing the number of piRNAs that can act to silence transposons | Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies.
Li C, Vagin VV, Lee S, Xu J, Ma S, Xi H, Seitz H, Horwich MD, Syrzycka M, Honda BM, Kittler EL, Zapp ML, Klattenhoff C, Schulz N, Theurkauf WE, Weng Z, Zamore PD., Free PMC Article | 01/21/2010 |
| The Drosophila homologue of protein methyltransferase 5 (dPRMT5, csul/dart5)methylates arginine in Drosophila Piwi, Ago3 and Aub proteins in vivo. Arginine methylation of Piwi proteins is required for Ago3 and Aub stability. | Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stability.
Kirino Y, Kim N, de Planell-Saguer M, Khandros E, Chiorean S, Klein PS, Rigoutsos I, Jongens TA, Mourelatos Z., Free PMC Article | 01/21/2010 |
| PIWI subfamily member AGO3 had Slicer activity in vitro; data support model in which formation of a 5' terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with Slicer activity of PIWI | A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila.
Gunawardane LS, Saito K, Nishida KM, Miyoshi K, Kawamura Y, Nagami T, Siomi H, Siomi MC. | 01/21/2010 |
| Study examined piwi-interacting RNAs (piRNAs) associated with each Drosophila Piwi protein and found that Piwi and Aubergine bind predominantly RNAs antisense to transposons, whereas Ago3 complexes contain predominantly sense piRNAs. | Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila.
Brennecke J, Aravin AA, Stark A, Dus M, Kellis M, Sachidanandam R, Hannon GJ. | 01/21/2010 |