| The Smc5/6 complex is a DNA loop-extruding motor. | The Smc5/6 complex is a DNA loop-extruding motor.
Pradhan B, Kanno T, Umeda Igarashi M, Loke MS, Baaske MD, Wong JSK, Jeppsson K, Björkegren C, Kim E., Free PMC Article | 05/15/2023 |
| Cryo-EM structure of the Smc5/6 holo-complex. | Cryo-EM structure of the Smc5/6 holo-complex.
Hallett ST, Campbell Harry I, Schellenberger P, Zhou L, Cronin NB, Baxter J, Etheridge TJ, Murray JM, Oliver AW., Free PMC Article | 11/19/2022 |
| Cryo-EM structure of DNA-bound Smc5/6 reveals DNA clamping enabled by multi-subunit conformational changes. | Cryo-EM structure of DNA-bound Smc5/6 reveals DNA clamping enabled by multi-subunit conformational changes.
Yu Y, Li S, Ser Z, Kuang H, Than T, Guan D, Zhao X, Patel DJ., Free PMC Article | 06/11/2022 |
| Smc5/6 in the rDNA modulates lifespan independently of Fob1. | Smc5/6 in the rDNA modulates lifespan independently of Fob1.
Moradi-Fard S, Mojumdar A, Chan M, Harkness TAA, Cobb JA., Free PMC Article | 01/1/2022 |
| Integrative analysis reveals unique structural and functional features of the Smc5/6 complex. | Integrative analysis reveals unique structural and functional features of the Smc5/6 complex.
Yu Y, Li S, Ser Z, Sanyal T, Choi K, Wan B, Kuang H, Sali A, Kentsis A, Patel DJ, Zhao X., Free PMC Article | 12/11/2021 |
| Nse5/6 inhibits the Smc5/6 ATPase and modulates DNA substrate binding. | Nse5/6 inhibits the Smc5/6 ATPase and modulates DNA substrate binding.
Taschner M, Basquin J, Steigenberger B, Schäfer IB, Soh YM, Basquin C, Lorentzen E, Räschle M, Scheltema RA, Gruber S., Free PMC Article | 12/4/2021 |
| Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex. | Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex.
Hallett ST, Schellenberger P, Zhou L, Beuron F, Morris E, Murray JM, Oliver AW., Free PMC Article | 06/19/2021 |
| Relocation of Collapsed Forks to the Nuclear Pore Complex Depends on Sumoylation of DNA Repair Proteins and Permits Rad51 Association. | Relocation of Collapsed Forks to the Nuclear Pore Complex Depends on Sumoylation of DNA Repair Proteins and Permits Rad51 Association.
Whalen JM, Dhingra N, Wei L, Zhao X, Freudenreich CH., Free PMC Article | 05/22/2021 |
| Smc5/6 functions with Sgs1-Top3-Rmi1 to complete chromosome replication at natural pause sites. | Smc5/6 functions with Sgs1-Top3-Rmi1 to complete chromosome replication at natural pause sites.
Agashe S, Joseph CR, Reyes TAC, Menolfi D, Giannattasio M, Waizenegger A, Szakal B, Branzei D., Free PMC Article | 04/24/2021 |
| Purified Smc5/6 Complex Exhibits DNA Substrate Recognition and Compaction. | Purified Smc5/6 Complex Exhibits DNA Substrate Recognition and Compaction.
Gutierrez-Escribano P, Hormeño S, Madariaga-Marcos J, Solé-Soler R, O'Reilly FJ, Morris K, Aicart-Ramos C, Aramayo R, Montoya A, Kramer H, Rappsilber J, Torres-Rosell J, Moreno-Herrero F, Aragon L., Free PMC Article | 01/16/2021 |
| Our findings thus indicate a direct role for SUMO in Pol2-mediated DNA synthesis and a molecular basis for Smc5/6-mediated regulation of genome stability | Sumoylation of the DNA polymerase ε by the Smc5/6 complex contributes to DNA replication.
Meng X, Wei L, Peng XP, Zhao X., Free PMC Article | 02/22/2020 |
| These results reveal a mechanism to enhance a SUMO E3 ligase activity by direct DNA binding and to restrict sumoylation in the vicinity of those Smc5/6-Nse2 molecules engaged on DNA. | DNA activates the Nse2/Mms21 SUMO E3 ligase in the Smc5/6 complex.
Varejão N, Ibars E, Lascorz J, Colomina N, Torres-Rosell J, Reverter D., Free PMC Article | 02/23/2019 |
| Data suggest that aSmc5/6 likely links rDNA stability to overall mitotic growth. | Acute Smc5/6 depletion reveals its primary role in rDNA replication by restraining recombination at fork pausing sites.
Peng XP, Lim S, Li S, Marjavaara L, Chabes A, Zhao X., Free PMC Article | 06/23/2018 |
| Our findings reveal a requirement for the Smc5/6 complex, including Mms21/Nse2 mediated sumoylation, and topoisomerase-1 (Top1), for maintaining stability of a chromosome having low origin density and suggest a functional cooperation between the Smc5/6 complex and Top1 in maintenance of topologically challenged chromosomes prone to replication fork collapse or accumulation of torsional stress | Genetic evidence for functional interaction of Smc5/6 complex and Top1 with spatial frequency of replication origins required for maintenance of chromosome stability.
Rai R, Laloraya S. | 04/7/2018 |
| Mph1 is a double-edged sword whose action at hybrids must be regulated by the Smc5/6 complex. This is underlined by the observation that simultaneous inactivation of RNase H2 and Smc5/6 results in Mph1-dependent synthetic lethality. | The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage.
Lafuente-Barquero J, Luke-Glaser S, Graf M, Silva S, Gómez-González B, Lockhart A, Lisby M, Aguilera A, Luke B., Free PMC Article | 03/3/2018 |
| nse3-1/ sir4 Delta double mutants show additive defects for telomere shortening and TPE indicating the contribution of Smc5/6 to telomere homeostasis is only in partial overlap with SIR factor silencing. These findings support a role for Smc5/6 in telomere maintenance that is separate from its canonical role(s) in HR-mediated events during replication and telomere elongation. | Smc5/6 Is a Telomere-Associated Complex that Regulates Sir4 Binding and TPE.
Moradi-Fard S, Sarthi J, Tittel-Elmer M, Lalonde M, Cusanelli E, Chartrand P, Cobb JA., Free PMC Article | 05/27/2017 |
| The chromosomal association of the Smc5 and Smc6 complex depends on cohesion and predicts the level of sister chromatid entanglement. | The chromosomal association of the Smc5/6 complex depends on cohesion and predicts the level of sister chromatid entanglement.
Jeppsson K, Carlborg KK, Nakato R, Berta DG, Lilienthal I, Kanno T, Lindqvist A, Brink MC, Dantuma NP, Katou Y, Shirahige K, Sjögren C., Free PMC Article | 04/30/2016 |
| Results show Smc5/6 essential roles and reveal that combined defects in DNA damage tolerance and pausing site-replication cause recombination-mediated DNA lesions. | Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance.
Menolfi D, Delamarre A, Lengronne A, Pasero P, Branzei D., Free PMC Article | 04/30/2016 |
| The Smc5/6-Mms21 complex operates as a large SUMO ligase in vivo during DNA repair. | ATPase-dependent control of the Mms21 SUMO ligase during DNA repair.
Bermúdez-López M, Pociño-Merino I, Sánchez H, Bueno A, Guasch C, Almedawar S, Bru-Virgili S, Garí E, Wyman C, Reverter D, Colomina N, Torres-Rosell J., Free PMC Article | 01/23/2016 |
| Smc5/6 and cohesin complexes are interrelated and cooperating factors that control chromosome dynamics throughout interphase. [review] | Functional interplay between cohesin and Smc5/6 complexes.
Tapia-Alveal C, Lin SJ, O'Connell MJ., Free PMC Article | 05/23/2015 |
| Smc5-Smc6 binds to a Mph1 region required for efficient fork regression, preventing assembly of Mph1 oligomers at the junction of DNA forks. | Restriction of replication fork regression activities by a conserved SMC complex.
Xue X, Choi K, Bonner JN, Chiba T, Kwon Y, Xu Y, Sanchez H, Wyman C, Niu H, Zhao X, Sung P., Free PMC Article | 02/28/2015 |
| the Smc5/6 protein complex is directly involved in meiotic recombination and suggest that Smc6 plays a key role in resolving recombination intermediates during meiosis, especially those that form between sister chromatids. | Inhibition of the Smc5/6 complex during meiosis perturbs joint molecule formation and resolution without significantly changing crossover or non-crossover levels.
Lilienthal I, Kanno T, Sjögren C., Free PMC Article | 05/17/2014 |
| Saccharomyces cerevisiae smc6 mutant survival, upon transient replication stress, can be improved by enhancing Mec1 checkpoint signaling. | DNA damage checkpoint and recombinational repair differentially affect the replication stress tolerance of Smc6 mutants.
Chen YH, Szakal B, Castellucci F, Branzei D, Zhao X., Free PMC Article | 03/1/2014 |
| Smc5/Smc6 complex binds single-stranded DNA and participates in DNA replication and repair. | A touching moment for Smc5/6: from ssDNA binding to repair.
Ohouo PY, Smolka MB. | 09/3/2011 |
| Smc5/6 does not simply restrain the recombinogenic activity of Mph1 via direct binding. | Homologous recombination-dependent rescue of deficiency in the structural maintenance of chromosomes (Smc) 5/6 complex.
Chavez A, Agrawal V, Johnson FB., Free PMC Article | 04/16/2011 |