| Site-specific Phosphorylation of Histone H3K36 Methyltransferase Set2p and Demethylase Jhd1p is Required for Stress Responses in Saccharomyces cerevisiae. | Site-specific Phosphorylation of Histone H3K36 Methyltransferase Set2p and Demethylase Jhd1p is Required for Stress Responses in Saccharomyces cerevisiae.
Separovich RJ, Wong MWM, Bartolec TK, Hamey JJ, Wilkins MR. | 05/14/2022 |
| An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation. | An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation.
Lerner AM, Hepperla AJ, Keele GR, Meriesh HA, Yumerefendi H, Restrepo D, Zimmerman S, Bear JE, Kuhlman B, Davis IJ, Strahl BD., Free PMC Article | 11/22/2021 |
| Core promoter activity contributes to chromatin-based regulation of internal cryptic promoters. | Core promoter activity contributes to chromatin-based regulation of internal cryptic promoters.
Lee BB, Woo H, Lee MK, Youn S, Lee S, Roe JS, Lee SY, Kim T., Free PMC Article | 10/9/2021 |
| Methylation of histone H3 at lysine 37 by Set1 and Set2 prevents spurious DNA replication. | Methylation of histone H3 at lysine 37 by Set1 and Set2 prevents spurious DNA replication.
Santos-Rosa H, Millán-Zambrano G, Han N, Leonardi T, Klimontova M, Nasiscionyte S, Pandolfini L, Tzelepis K, Bartke T, Kouzarides T., Free PMC Article | 07/31/2021 |
| Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence. | Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence.
Wagner T, Pérez-Martínez L, Schellhaas R, Barrientos-Moreno M, Öztürk M, Prado F, Butter F, Luke B., Free PMC Article | 01/23/2021 |
| The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae. | The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae.
Reim NI, Chuang J, Jain D, Alver BH, Park PJ, Winston F., Free PMC Article | 11/21/2020 |
| the histone H3K4 methyltransferase Set1, and the histone H3K36 methyltransferase Set2, control choice ofpolyadenylation site in Saccharomyces cerevisiae | Regulation of alternative polyadenylation in the yeast Saccharomyces cerevisiae by histone H3K4 and H3K36 methyltransferases.
Kaczmarek Michaels K, Mohd Mostafa S, Ruiz Capella J, Moore CL., Free PMC Article | 08/22/2020 |
| A conserved genetic interaction between Spt6 and Set2 regulates H3K36 methylation. | A conserved genetic interaction between Spt6 and Set2 regulates H3K36 methylation.
Gopalakrishnan R, Marr SK, Kingston RE, Winston F., Free PMC Article | 11/30/2019 |
| Set2 methyltransferase facilitates cell cycle progression by maintaining transcriptional fidelity. | Set2 methyltransferase facilitates cell cycle progression by maintaining transcriptional fidelity.
Dronamraju R, Jha DK, Eser U, Adams AT, Dominguez D, Choudhury R, Chiang YC, Rathmell WK, Emanuele MJ, Churchman LS, Strahl BD., Free PMC Article | 07/6/2019 |
| report that both methyltransferases can be UV cross-linked to RNA in vivo High-throughput sequencing of the bound RNAs revealed strong Set1 enrichment near the transcription start site, whereas Set2 was distributed along pre-mRNAs | RNA Binding by Histone Methyltransferases Set1 and Set2.
Sayou C, Millán-Zambrano G, Santos-Rosa H, Petfalski E, Robson S, Houseley J, Kouzarides T, Tollervey D., Free PMC Article | 09/23/2017 |
| one of these mutations (R2510H), located in the Set2 Rpb1 interaction domain, did not result in an observable defect in SETD2 enzymatic function, a second mutation in the catalytic domain of this enzyme (R1625C) resulted in a complete loss of histone H3 Lys-36 trimethylation (H3K36me3). | Structure/Function Analysis of Recurrent Mutations in SETD2 Protein Reveals a Critical and Conserved Role for a SET Domain Residue in Maintaining Protein Stability and Histone H3 Lys-36 Trimethylation.
Hacker KE, Fahey CC, Shinsky SA, Chiang YJ, DiFiore JV, Jha DK, Vo AH, Shavit JA, Davis IJ, Strahl BD, Rathmell WK., Free PMC Article | 05/20/2017 |
| Set2 suppresses the restoration of unstable ON telomeres to the stable OFF state and promotes cellular aging. | Loss of the Set2 histone methyltransferase increases cellular lifespan in yeast cells.
Ryu HY, Rhie BH, Ahn SH. | 06/14/2014 |
| Set2 functions both to suppress the incorporation of acetylated histones and to signal for the deacetylation of these histones in transcribed genes. By suppressing spurious cryptic transcripts from initiating within ORFs, this pathway is essential to maintain the accuracy of transcription by RNA polymerase II. | Set2 methylation of histone H3 lysine 36 suppresses histone exchange on transcribed genes.
Venkatesh S, Smolle M, Li H, Gogol MM, Saint M, Kumar S, Natarajan K, Workman JL. | 11/17/2012 |
| RNA polymerase II carboxyl-terminal domain phosphorylation regulates protein stability of the Set2 methyltransferase and histone H3 di- and trimethylation at lysine 36. | RNA polymerase II carboxyl-terminal domain phosphorylation regulates protein stability of the Set2 methyltransferase and histone H3 di- and trimethylation at lysine 36.
Fuchs SM, Kizer KO, Braberg H, Krogan NJ, Strahl BD., Free PMC Article | 03/24/2012 |
| Mapping of interaction domains revealed the importance of the SET core domain which is necessary and sufficient for binding Ino2. | Mediator subunits and histone methyltransferase Set2 contribute to Ino2-dependent transcriptional activation of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.
Dettmann A, Jäschke Y, Triebel I, Bogs J, Schröder I, Schüller HJ. | 05/3/2010 |
| Asf1 stimulates Set2 occupancy of the coding region of a highly transcribed gene by a mechanism that depends on Asf1 binding to H3/H4. | Asf1 can promote trimethylation of H3 K36 by Set2.
Lin LJ, Minard LV, Johnston GC, Singer RA, Schultz MC., Free PMC Article | 03/29/2010 |
| Data suggest that Set2 is controlled by intratail interactions which can be influenced by modifications and changes to the structure of the H3 tail to control the dynamics and localization of methylation during elongation. | Set2-dependent K36 methylation is regulated by novel intratail interactions within H3.
Psathas JN, Zheng S, Tan S, Reese JC., Free PMC Article | 01/21/2010 |
| Results suggest that suppression of cryptic transcription via the Set2/Rpd3S pathway occurs independent of gene length or transcriptional frequency. | The Set2/Rpd3S pathway suppresses cryptic transcription without regard to gene length or transcription frequency.
Lickwar CR, Rao B, Shabalin AA, Nobel AB, Strahl BD, Lieb JD., Free PMC Article | 01/21/2010 |
| Loss of Set2p or Rpd3p substantially elevated HIS4 hotspot activity, and loss of Hda1p had a smaller stimulatory effect | The histone methylase Set2p and the histone deacetylase Rpd3p repress meiotic recombination at the HIS4 meiotic recombination hotspot in Saccharomyces cerevisiae.
Merker JD, Dominska M, Greenwell PW, Rinella E, Bouck DC, Shibata Y, Strahl BD, Mieczkowski P, Petes TD., Free PMC Article | 01/21/2010 |
| S-phase progression is suppressed by set2 or chd1 mutations, suggesting that Set2 and Chd1 have specific roles in negatively regulating DNA replication. | A role for Chd1 and Set2 in negatively regulating DNA replication in Saccharomyces cerevisiae.
Biswas D, Takahata S, Xin H, Dutta-Biswas R, Yu Y, Formosa T, Stillman DJ., Free PMC Article | 01/21/2010 |
| A novel domain in Set2 has been defined, which interacts with RNA polymerase II and is responsible for the methylation of histone 3 at K36 required for the transcription elongation process. | A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.
Kizer KO, Phatnani HP, Shibata Y, Hall H, Greenleaf AL, Strahl BD., Free PMC Article | 01/21/2010 |
| yFACT and Set2 oppose one another during transcriptional initiation at a step involving DNA binding by TBP and TFIIA. | Opposing roles for Set2 and yFACT in regulating TBP binding at promoters.
Biswas D, Dutta-Biswas R, Mitra D, Shibata Y, Strahl BD, Formosa T, Stillman DJ., Free PMC Article | 01/21/2010 |
| SGV1 is required for the normal pattern of histone methylation by SET2. | The BUR1 cyclin-dependent protein kinase is required for the normal pattern of histone methylation by SET2.
Chu Y, Sutton A, Sternglanz R, Prelich G., Free PMC Article | 01/21/2010 |
| The solution structure of the yeast Set2 SRI domain reveals a novel carboxyl-terminal domain binding fold consisting of a left-handed three-helix bundle | Structure and carboxyl-terminal domain (CTD) binding of the Set2 SRI domain that couples histone H3 Lys36 methylation to transcription.
Vojnic E, Simon B, Strahl BD, Sattler M, Cramer P. | 01/21/2010 |