| Molecular basis for processing of topoisomerase 1-triggered DNA damage by Apn2/APE2. | Molecular basis for processing of topoisomerase 1-triggered DNA damage by Apn2/APE2.
Williams JS, Wojtaszek JL, Appel DC, Krahn J, Wallace BD, Walsh E, Kunkel TA, Williams RS., Free PMC Article | 10/15/2022 |
| Topoisomerases Modulate the Timing of Meiotic DNA Breakage and Chromosome Morphogenesis in Saccharomyces cerevisiae. | Topoisomerases Modulate the Timing of Meiotic DNA Breakage and Chromosome Morphogenesis in Saccharomyces cerevisiae.
Heldrich J, Sun X, Vale-Silva LA, Markowitz TE, Hochwagen A., Free PMC Article | 04/3/2021 |
| Fob1p recruits DNA topoisomerase I to ribosomal genes locus and contributes to its transcriptional silencing maintenance | Fob1p recruits DNA topoisomerase I to ribosomal genes locus and contributes to its transcriptional silencing maintenance.
Di Felice F, Egidi A, D'Alfonso A, Camilloni G. | 01/11/2020 |
| Studies demonstrate opposing roles of Top1 activity in either promoting or destabilizing genome integrity. Resolution of transcription-associated negative supercoils, for example, prevents pairing of the nascent RNA with the DNA template (R-loops) as well as DNA secondary structure formation. Reduced Top1 levels thus enhance CAG repeat contraction, somatic hypermutation, and class switch recombination. | Topoisomerase I and Genome Stability: The Good and the Bad.
Cho JE, Jinks-Robertson S. | 07/7/2018 |
| Our findings reveal a requirement for the Smc5/6 complex, including Mms21/Nse2 mediated sumoylation, and topoisomerase-1 (Top1), for maintaining stability of a chromosome having low origin density and suggest a functional cooperation between the Smc5/6 complex and Top1 in maintenance of topologically challenged chromosomes prone to replication fork collapse or accumulation of torsional stress | Genetic evidence for functional interaction of Smc5/6 complex and Top1 with spatial frequency of replication origins required for maintenance of chromosome stability.
Rai R, Laloraya S. | 04/7/2018 |
| Our findings reveal a functional interplay between Mms21 and Top1 in maintenance of longer chromosomes, and suggest that lack of sumoylation of Mms21 targets coupled with Top1 deficiency is a crucial requirement for accurate inheritance of longer chromosomes. | Interplay between Top1 and Mms21/Nse2 mediated sumoylation in stable maintenance of long chromosomes.
Mahendrawada L, Rai R, Kothiwal D, Laloraya S. | 04/7/2018 |
| Too much, as well as too little of Top1 is detrimental to eukaryotic genomes, and that CPT has destabilizing effects that extend beyond those associated with DSB formation. | Effects of camptothecin or TOP1 overexpression on genetic stability in Saccharomyces cerevisiae.
Sloan R, Huang SN, Pommier Y, Jinks-Robertson S., Free PMC Article | 12/2/2017 |
| The authors demonstrate biochemically that irreversible double-strand breaks are generated by subsequent Top1 cleavage on the opposite strand from the Top1-induced DNA nicks at ribonucleotide sites. | Topoisomerase I-mediated cleavage at unrepaired ribonucleotides generates DNA double-strand breaks.
Huang SN, Williams JS, Arana ME, Kunkel TA, Pommier Y., Free PMC Article | 07/8/2017 |
| These genetic and biochemical studies support a model whereby Top1p recruits Sir2p to the rDNA and clarifies a structural role of DNA topoisomerase I in the epigenetic regulation of rDNA, independent of its known catalytic activity. | Molecular Mechanism of DNA Topoisomerase I-Dependent rDNA Silencing: Sir2p Recruitment at Ribosomal Genes.
D'Alfonso A, Di Felice F, Carlini V, Wright CM, Hertz MI, Bjornsti MA, Camilloni G. | 07/1/2017 |
| The role of topoisomerase I in suppressing genome instability associated with a highly transcribed guanine-rich sequence is not restricted to preventing RNA:DNA hybrid accumulation. | The role of topoisomerase I in suppressing genome instability associated with a highly transcribed guanine-rich sequence is not restricted to preventing RNA:DNA hybrid accumulation.
Yadav P, Owiti N, Kim N., Free PMC Article | 06/28/2016 |
| top1 mutants had greatly elevated levels of instability within the tandemly-repeated ribosomal RNA genes. | Genome-destabilizing effects associated with top1 loss or accumulation of top1 cleavage complexes in yeast.
Andersen SL, Sloan RS, Petes TD, Jinks-Robertson S., Free PMC Article | 03/26/2016 |
| Top1 is a critical factor in suppression of instability associated with potential G4 DNA-forming sequences. | Topoisomerase I plays a critical role in suppressing genome instability at a highly transcribed G-quadruplex-forming sequence.
Yadav P, Harcy V, Argueso JL, Dominska M, Jinks-Robertson S, Kim N., Free PMC Article | 01/30/2016 |
| analysis of how human and yeast DNA topoisomerase I domain interactions impact enzyme activity and sensitivity to camptothecin | DNA topoisomerase I domain interactions impact enzyme activity and sensitivity to camptothecin.
Wright CM, van der Merwe M, DeBrot AH, Bjornsti MA., Free PMC Article | 08/8/2015 |
| Top1 reversibly cleaves genomic ribonucleotides with formation of a ribonucleotide 2',3'-cyclic phosphate.Top1 cleaves two nucleotides upstream of the ribonucleotide 2',3'-cyclic phosphate to yield a covalent Top1-DNA complex. | Error-free and mutagenic processing of topoisomerase 1-provoked damage at genomic ribonucleotides.
Sparks JL, Burgers PM., Free PMC Article | 07/25/2015 |
| Nsr1 and its interacting partner Top1 are polyphosphorylated. | Protein polyphosphorylation of lysine residues by inorganic polyphosphate.
Azevedo C, Livermore T, Saiardi A. | 06/20/2015 |
| Top1-dependent deletions are initiated either by Top1 cleavage complex trapping or by Top1 incision at ribonucleoside monophosphates in DNA. | Two distinct mechanisms of Topoisomerase 1-dependent mutagenesis in yeast.
Cho JE, Kim N, Li YC, Jinks-Robertson S., Free PMC Article | 08/10/2013 |
| Data show that Top2 is required redundantly with Top1 for optimal recruitment of RNA polymerase II at their promoters. | Topoisomerase II binds nucleosome-free DNA and acts redundantly with topoisomerase I to enhance recruitment of RNA Pol II in budding yeast.
Sperling AS, Jeong KS, Kitada T, Grunstein M., Free PMC Article | 10/29/2011 |
| study demonstrates that rNMP (ribonucleoside monophosphates)-associated deletion formation requires the activity of Top1; studies extend the role of Top1 to include the processing of rNMPs in genomic DNA into irreversible single-strand breaks | Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I.
Kim N, Huang SN, Williams JS, Li YC, Clark AB, Cho JE, Kunkel TA, Pommier Y, Jinks-Robertson S., Free PMC Article | 07/9/2011 |
| analysis of unavoidable conflicts between Top1p and the transcriptional machinery and their potential impact on genome stability | Topoisomerase 1 provokes the formation of short deletions in repeated sequences upon high transcription in Saccharomyces cerevisiae.
Takahashi DT, Burguiere-Slezak G, Van der Kemp PA, Boiteux S., Free PMC Article | 02/26/2011 |
| Loss of Top1 enhances inherent R-loop formation, particularly over the 5' region of the rDNA, imposing persistent transcription blocks when RNase H is limiting. | Loss of Topoisomerase I leads to R-loop-mediated transcriptional blocks during ribosomal RNA synthesis.
El Hage A, French SL, Beyer AL, Tollervey D., Free PMC Article | 08/16/2010 |
| Disulfide cross-links reveal conserved features of DNA topoisomerase I architecture and a role for the N terminus in clamp closure | Disulfide cross-links reveal conserved features of DNA topoisomerase I architecture and a role for the N terminus in clamp closure.
Palle K, Pattarello L, van der Merwe M, Losasso C, Benedetti P, Bjornsti MA., Free PMC Article | 01/21/2010 |
| Top1p can optimize transcript levels for cell growth in exponentially growing cells under a synthetic medium with glucose | Global transcription regulation by DNA topoisomerase I in exponentially growing Saccharomyces cerevisiae cells: activation of telomere-proximal genes by TOP1 deletion.
Lotito L, Russo A, Chillemi G, Bueno S, Cavalieri D, Capranico G. | 01/21/2010 |
| Data show that mutation of a conserved active site residue converts tyrosyl-DNA phosphodiesterase I into a DNA topoisomerase I-dependent poison. | Mutation of a conserved active site residue converts tyrosyl-DNA phosphodiesterase I into a DNA topoisomerase I-dependent poison.
He X, van Waardenburg RCAM, Babaoglu K, Price AC, Nitiss KC, Nitiss JL, Bjornsti MA, White SW. | 01/21/2010 |
| data are consistent with a coordinated action of Top1 and Top2 in counteracting the accumulation of torsional stress and sister chromatid entanglement at replication forks | Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation.
Bermejo R, Doksani Y, Capra T, Katou YM, Tanaka H, Shirahige K, Foiani M., Free PMC Article | 01/21/2010 |
| Changes in the flexibility or orientation of the linker alter the geometry of the active site and thereby the kinetics of DNA cleavage/religation. | Alterations in linker flexibility suppress DNA topoisomerase I mutant-induced cell lethality.
Losasso C, Cretaio E, Palle K, Pattarello L, Bjornsti MA, Benedetti P. | 01/21/2010 |