| Rif2 interaction with Rad50 counteracts Tel1 functions in checkpoint signalling and DNA tethering by releasing Tel1 from MRX binding. | Rif2 interaction with Rad50 counteracts Tel1 functions in checkpoint signalling and DNA tethering by releasing Tel1 from MRX binding.
Pizzul P, Casari E, Rinaldi C, Gnugnoli M, Mangiagalli M, Tisi R, Longhese MP., Free PMC Article | 03/25/2024 |
| Xrs2/NBS1 promote end-bridging activity of the MRE11-RAD50 complex. | Xrs2/NBS1 promote end-bridging activity of the MRE11-RAD50 complex.
Möller C, Sharma R, Öz R, Reginato G, Cannavo E, Ceppi I, Sriram KK, Cejka P, Westerlund F. | 02/8/2024 |
| Inhibition of MRN activity by a telomere protein motif. | Inhibition of MRN activity by a telomere protein motif.
Khayat F, Cannavo E, Alshmery M, Foster WR, Chahwan C, Maddalena M, Smith C, Oliver AW, Watson AT, Carr AM, Cejka P, Bianchi A., Free PMC Article | 07/24/2021 |
| A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization. | A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization.
Forey R, Barthe A, Tittel-Elmer M, Wery M, Barrault MB, Ducrot C, Seeber A, Krietenstein N, Szachnowski U, Skrzypczak M, Ginalski K, Rowicka M, Cobb JA, Rando OJ, Soutourina J, Werner M, Dubrana K, Gasser SM, Morillon A, Pasero P, Lengronne A, Poli J., Free PMC Article | 02/2/2021 |
| Effect of BRCA1 missense variants on gene reversion in DNA double-strand break repair mutants and cell cycle-arrested cells of Saccharomyces cerevisiae. | Effect of BRCA1 missense variants on gene reversion in DNA double-strand break repair mutants and cell cycle-arrested cells of Saccharomyces cerevisiae.
Lodovichi S, Bellè F, Cervelli T, Lorenzoni A, Maresca L, Cozzani C, Caligo MA, Galli A. | 01/2/2021 |
| Here we describe two separation-of-functions alleles, mre11-S499P and rad50-A78T, which we show to specifically affect Tel1 activation without impairing MRX functions in DSB repair. Both Mre11-S499P and Rad50-A78T reduce Tel1-MRX interaction leading to poor Tel1 association at DSBs and consequent loss of Tel1 activation | The ATP-bound conformation of the Mre11-Rad50 complex is essential for Tel1/ATM activation.
Cassani C, Vertemara J, Bassani M, Marsella A, Tisi R, Zampella G, Longhese MP., Free PMC Article | 10/12/2019 |
| Data show that ssDNA endodeoxyribonuclease SAE2 (Sae2) cooperates with the Mre11-Rad50-Xrs2 (MRX) complex to initiate resection of DNA double-strand breaks (DSBs) and to maintain the DSB ends in close proximity to allow their repair. | Structurally distinct Mre11 domains mediate MRX functions in resection, end-tethering and DNA damage resistance.
Cassani C, Gobbini E, Vertemara J, Wang W, Marsella A, Sung P, Tisi R, Zampella G, Longhese MP., Free PMC Article | 07/27/2019 |
| Phosphorylation of ssDNA endodeoxyribonuclease SAE2 (Sae2)does not affect its overall capacity to interact with Mre11-Rad50-Xrs2 (MRX), it controls the specific physical and functional interaction with the MRX subunit Rad50, which is a prerequisite for the regulation of the Mre11 nuclease. | Regulatory control of DNA end resection by Sae2 phosphorylation.
Cannavo E, Johnson D, Andres SN, Kissling VM, Reinert JK, Garcia V, Erie DA, Hess D, Thomä NH, Enchev RI, Peter M, Williams RS, Neale MJ, Cejka P., Free PMC Article | 01/12/2019 |
| Xrs2 dependent and independent functions of the Mre11-Rad50 complex orchestrating the cellular response to double-strand DNA breaks have been described. | Xrs2 Dependent and Independent Functions of the Mre11-Rad50 Complex.
Oh J, Al-Zain A, Cannavo E, Cejka P, Symington LS., Free PMC Article | 09/9/2017 |
| The Saccharomyces cerevisiae Mre11-Rad50-Xrs2 complex promotes trinucleotide repeat expansions independently of homologous recombination. | The Saccharomyces cerevisiae Mre11-Rad50-Xrs2 complex promotes trinucleotide repeat expansions independently of homologous recombination.
Ye Y, Kirkham-McCarthy L, Lahue RS. | 05/20/2017 |
| Mre11 recruits conjugated SUMO moieties to facilitate the assembly and function of the Mre11-Rad50-Xrs2 complex. | S. cerevisiae Mre11 recruits conjugated SUMO moieties to facilitate the assembly and function of the Mre11-Rad50-Xrs2 complex.
Chen YJ, Chuang YC, Chuang CN, Cheng YH, Chang CR, Leng CH, Wang TF., Free PMC Article | 08/13/2016 |
| Data suggest that the major function of Sae2 in response to DNA double-strand breaks (DSBs) is to actively remove Mre11-Rad50-Xrs2 from break ends and prevent Rad53 in response to DSBs. | Sae2 promotes DNA damage resistance by removing the Mre11-Rad50-Xrs2 complex from DNA and attenuating Rad53 signaling.
Chen H, Donnianni RA, Handa N, Deng SK, Oh J, Timashev LA, Kowalczykowski SC, Symington LS., Free PMC Article | 07/25/2015 |
| To our surprise, genes encoding the Mre11-Rad50-Xrs2 (MRX) complex, which are also required for homologous recombination, are epistatic to TLS mutations | The Mre11-Rad50-Xrs2 complex is required for yeast DNA postreplication repair.
Ball LG, Hanna MD, Lambrecht AD, Mitchell BA, Ziola B, Cobb JA, Xiao W., Free PMC Article | 07/4/2015 |
| Data demonstrate that the dimerization state of the Rad50 hook domain exerts diverse influences on Mre11 complex function. | Interdependence of the rad50 hook and globular domain functions.
Hohl M, Kochańczyk T, Tous C, Aguilera A, Krężel A, Petrini JH., Free PMC Article | 04/18/2015 |
| Sae2 promotes dsDNA-specific endonuclease activity by the Mre11 subunit within the Mre11-Rad50-Xrs2 (MRX) complex | Sae2 promotes dsDNA endonuclease activity within Mre11-Rad50-Xrs2 to resect DNA breaks.
Cannavo E, Cejka P. | 10/25/2014 |
| Processing of DNA double-stranded breaks and intermediates of recombination and repair by Saccharomyces cerevisiae Mre11 and its stimulation by Rad50, Xrs2, and Sae2 proteins. | Processing of DNA double-stranded breaks and intermediates of recombination and repair by Saccharomyces cerevisiae Mre11 and its stimulation by Rad50, Xrs2, and Sae2 proteins.
Ghodke I, Muniyappa K., Free PMC Article | 06/15/2013 |
| A pivotal role for the MRX (Mre11, Rad50, Xrs2) complex for fork integrity at replication forks. | MRX protects fork integrity at protein-DNA barriers, and its absence causes checkpoint activation dependent on chromatin context.
Bentsen IB, Nielsen I, Lisby M, Nielsen HB, Gupta SS, Mundbjerg K, Andersen AH, Bjergbaek L., Free PMC Article | 05/4/2013 |
| cohesin loading at replication sites depends on the structural features of Rad50 that are important for bridging sister chromatids, including the CXXC hook domain and the length of the coiled-coil extensions | Cohesin association to replication sites depends on rad50 and promotes fork restart.
Tittel-Elmer M, Lengronne A, Davidson MB, Bacal J, François P, Hohl M, Petrini JHJ, Pasero P, Cobb JA., Free PMC Article | 01/26/2013 |
| deletion of RAD50 may block the major pathway of nonhomologous integration into a non-repetitive chromosomal locus; Rad50 may be involved in facilitating nonhomologous integration of both ends of the integrating DNA into a single chromosomal locus | Effect of rad50 mutation on illegitimate recombination in Saccharomyces cerevisiae.
Chan CY, Zhu J, Schiestl RH. | 09/3/2011 |
| Study established that Dna2, Sgs1 and RPA constitute a minimal protein complex capable of DNA resection in vitro; in addition it was found that both the Top3 and Rmi1 complex and the Mre11-Rad50-Xrs2 complex have important roles as stimulatory components. | DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2.
Cejka P, Cannavo E, Polaczek P, Masuda-Sasa T, Pokharel S, Campbell JL, Kowalczykowski SC., Free PMC Article | 09/27/2010 |
| a direct role for the Mre11-Rad50-Xrs2 complex in the base excision repair process, which contributes to resistance against base-damaging agents and to the avoidance of mutations | A novel function for the Mre11-Rad50-Xrs2 complex in base excision repair.
Steininger S, Ahne F, Winkler K, Kleinschmidt A, Eckardt-Schupp F, Moertl S., Free PMC Article | 05/3/2010 |
| The Rad50/Mre11/Xrs2 complex is responsible for rapid processing of most damaged ends into substrates that subsequently undergo recombinational repair. | RAD50 is required for efficient initiation of resection and recombinational repair at random, gamma-induced double-strand break ends.
Westmoreland J, Ma W, Yan Y, Van Hulle K, Malkova A, Resnick MA., Free PMC Article | 01/21/2010 |
| The Mre11/Rad50/Xrs2 (MRX) complex is essential for joining of incompatible ends by non-homologous end-joining during DNA repair, and the adenosine triphosphate (ATP)-dependent activities of Rad50 are critical for this process. | The Mre11/Rad50/Xrs2 complex and non-homologous end-joining of incompatible ends in S. cerevisiae.
Zhang X, Paull TT. | 01/21/2010 |
| Recombinant Sae2 binds DNA and exhibits endonuclease activity on single-stranded DNA independently of Mre11/Rad50 complexes, but hairpin DNA structures are cleaved cooperatively in the presence of Mre11/Rad50 or Mre11/Rad50/Xrs2. | Sae2 is an endonuclease that processes hairpin DNA cooperatively with the Mre11/Rad50/Xrs2 complex.
Lengsfeld BM, Rattray AJ, Bhaskara V, Ghirlando R, Paull TT., Free PMC Article | 01/21/2010 |
| Data show that the Saccharomyces cerevisiae MRX complex, or its subunits Mre11/Rad50/Xrs2, prefer G-quadruplex DNA much more than telomeric single-stranded or double-stranded DNA, implicating the possible existence of this DNA structure in vivo. | The characterization of Saccharomyces cerevisiae Mre11/Rad50/Xrs2 complex reveals that Rad50 negatively regulates Mre11 endonucleolytic but not the exonucleolytic activity.
Ghosal G, Muniyappa K. | 01/21/2010 |