| The authors identified mutations in a conserved and essential Mcm4 motif that permit loading of two Mcm2-7 complexes but are defective for double-hexamer formation. | A conserved Mcm4 motif is required for Mcm2-7 double-hexamer formation and origin DNA unwinding.
Champasa K, Blank C, Friedman LJ, Gelles J, Bell SP., Free PMC Article | 02/8/2020 |
| Sld3 binds specifically to DDK-phosphorylated peptides from two MCM subunits (Mcm4, 6) and then recruits Cdc45. | Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation.
Deegan TD, Yeeles JT, Diffley JF., Free PMC Article | 07/8/2017 |
| The Dbf4-dependent Cdc7 kinase (DDK), cyclin-dependent kinase (CDK), and Mec1 all target the structurally disordered N-terminal serine/threonine-rich domain of Mcm4, a subunit of the mini-chromosome maintenance replicative helicase complex. | Domain within the helicase subunit Mcm4 integrates multiple kinase signals to control DNA replication initiation and fork progression.
Sheu YJ, Kinney JB, Lengronne A, Pasero P, Stillman B., Free PMC Article | 07/5/2014 |
| constitutive overexpression of each individual MCM subunit was examined, and genotoxic sensitivity was found to be specific to Mcm2 or Mcm4 overexpression, further pointing to the importance of the DDK-MCM ring interaction. | Dbf4 and Cdc7 proteins promote DNA replication through interactions with distinct Mcm2-7 protein subunits.
Ramer MD, Suman ES, Richter H, Stanger K, Spranger M, Bieberstein N, Duncker BP., Free PMC Article | 08/10/2013 |
| A defective MCM helicase causes genetic instability only in particular cell types. | Ploidy dictates repair pathway choice under DNA replication stress.
Li XC, Tye BK., Free PMC Article | 08/20/2011 |
| The minimal functional unit of Mcm4/6/7 is a hexamer | The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function.
Ma X, Stead BE, Rezvanpour A, Davey MJ., Free PMC Article | 01/15/2011 |
| studies establish that the eukaryote-specific NSD of Mcm4 has evolved to integrate several protein kinase regulatory signals for progression through S phase | The Dbf4-Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4.
Sheu YJ, Stillman B., Free PMC Article | 02/15/2010 |
| DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA. | Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
Remus D, Beuron F, Tolun G, Griffith JD, Morris EP, Diffley JF., Free PMC Article | 01/21/2010 |
| using Saccharomyces cerevisiae containing a mcm4 helicase allele that was shown to cause cancer in mice. | Aneuploidy and improved growth are coincident but not causal in a yeast cancer model.
Li XC, Schimenti JC, Tye BK., Free PMC Article | 01/21/2010 |
| The Mcm4/Mcm7 and Mcm4/Mcm6/Mcm7 assemblies can open to load onto circular DNA to initiate unwinding. | Mcm subunits can assemble into two different active unwinding complexes.
Kanter DM, Bruck I, Kaplan DL., Free PMC Article | 01/21/2010 |
| Budding yeast Mcm4 is phosphorylated in vivo during S phase in a manner dependent on the presence of five CDK phosphoacceptor residues within the N-terminal domain of Mcm4. | Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation of yeast Mcm4 N-terminal domain.
Devault A, Gueydon E, Schwob E., Free PMC Article | 01/21/2010 |