| Exploring the removal of Spo11 and topoisomerases from DNA breaks in S. cerevisiae by human Tyrosyl DNA Phosphodiesterase 2. | Exploring the removal of Spo11 and topoisomerases from DNA breaks in S. cerevisiae by human Tyrosyl DNA Phosphodiesterase 2.
Johnson D, Allison RM, Cannavo E, Cejka P, Harper JA, Neale MJ. | 09/21/2024 |
| Physical interaction with Spo11 mediates the localisation of Mre11 to chromatin in meiosis and promotes its nuclease activity. | Physical interaction with Spo11 mediates the localisation of Mre11 to chromatin in meiosis and promotes its nuclease activity.
Aithal R, Nangalia K, Spirek M, Chen D, Klein F, Krejci L., Free PMC Article | 05/30/2024 |
| Chromosome-dependent aneuploid formation in Spo11-less meiosis. | Chromosome-dependent aneuploid formation in Spo11-less meiosis.
Kawashima Y, Oda AH, Hikida Y, Ohta K., Free PMC Article | 02/24/2023 |
| Concerted cutting by Spo11 illuminates meiotic DNA break mechanics. | Concerted cutting by Spo11 illuminates meiotic DNA break mechanics.
Johnson D, Crawford M, Cooper T, Claeys Bouuaert C, Keeney S, Llorente B, Garcia V, Neale MJ., Free PMC Article | 01/15/2022 |
| Spo11 generates gaps through concerted cuts at sites of topological stress. | Spo11 generates gaps through concerted cuts at sites of topological stress.
Prieler S, Chen D, Huang L, Mayrhofer E, Zsótér S, Vesely M, Mbogning J, Klein F. | 01/15/2022 |
| Structural and functional characterization of the Spo11 core complex. | Structural and functional characterization of the Spo11 core complex.
Claeys Bouuaert C, Tischfield SE, Pu S, Mimitou EP, Arias-Palomo E, Berger JM, Keeney S., Free PMC Article | 03/13/2021 |
| Dual roles of yeast Rad51 N-terminal domain in repairing DNA double-strand breaks. | Dual roles of yeast Rad51 N-terminal domain in repairing DNA double-strand breaks.
Woo TT, Chuang CN, Higashide M, Shinohara A, Wang TF., Free PMC Article | 10/24/2020 |
| A tel1Delta mutant has globally increased amounts of Spo11-oligonucleotide complexes and altered Spo11-oligonucleotide lengths, consistent with conserved roles for Tel1 in control of DSB number and processing..We further find that effects of Tel1 are distinct but partially overlapping with previously described contributions of the recombination regulator Cst9 (also known as Zip3). | Numerical and spatial patterning of yeast meiotic DNA breaks by Tel1.
Mohibullah N, Keeney S., Free PMC Article | 01/6/2018 |
| Data show that increases in meiotic double-strand breaks and crossover frequencies can be programmed to arbitrary genomic sites by fusion of the Spo11 protein to various DNA-recognition domains. | Programming sites of meiotic crossovers using Spo11 fusion proteins.
Sarno R, Vicq Y, Uematsu N, Luka M, Lapierre C, Carroll D, Bastianelli G, Serero A, Nicolas A., Free PMC Article | 12/2/2017 |
| we found a role for the meiotic bouquet in establishing the size dependence of centromere coupling, as abolishing bouquet (using the bouquet-defective spo11 ndj1 mutant) reduces it. Coupling in spo11 ndj1 rather follows telomere clustering preferences. We propose that a chromosome size preference for centromere coupling helps establish efficient homolog recognition. | Multiple Pairwise Analysis of Non-homologous Centromere Coupling Reveals Preferential Chromosome Size-Dependent Interactions and a Role for Bouquet Formation in Establishing the Interaction Pattern.
Lefrançois P, Rockmill B, Xie P, Roeder GS, Snyder M., Free PMC Article | 04/29/2017 |
| We examined fine-scale DSB distributions in TF mutant strains by deep sequencing oligonucleotides that remain covalently bound to Spo11 as a byproduct of DSB formation, mapped Bas1 and Ino4 binding sites in meiotic cells | High-Resolution Global Analysis of the Influences of Bas1 and Ino4 Transcription Factors on Meiotic DNA Break Distributions in Saccharomyces cerevisiae.
Zhu X, Keeney S., Free PMC Article | 07/16/2016 |
| These findings provide an explanation for the unexpectedly low prototroph levels exhibited by spo11 hypomorphs and have important implications for genetic studies that assume an unbiased recovery of prototrophs | High throughput sequencing reveals alterations in the recombination signatures with diminishing Spo11 activity.
Rockmill B, Lefrançois P, Voelkel-Meiman K, Oke A, Roeder GS, Fung JC., Free PMC Article | 02/21/2015 |
| Study shows that Spo11-accessory proteins Rec114, Mer2, and Mei4 stably interact with chromosome axis sequences, upon phosphorylation of Mer2 by S phase Cdk. | Spo11-accessory proteins link double-strand break sites to the chromosome axis in early meiotic recombination.
Panizza S, Mendoza MA, Berlinger M, Huang L, Nicolas A, Shirahige K, Klein F. | 10/1/2011 |
| How to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells. | End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae.
Neale MJ, Keeney S., Free PMC Article | 01/21/2010 |
| Results suggest that Rec8 would prearrange the distribution of Spo11 along chromosomes and will provide clues to understanding temporal and spatial regulation of DSB formation. | Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomes.
Kugou K, Fukuda T, Yamada S, Ito M, Sasanuma H, Mori S, Katou Y, Itoh T, Matsumoto K, Shibata T, Shirahige K, Ohta K., Free PMC Article | 01/21/2010 |
| These results, using the Gal4BD-Spo11 fusion protein, demonstrate that Spo11 itself has sequence preference and contributes to the choice of DNA double-strand break positions. | Locally, meiotic double-strand breaks targeted by Gal4BD-Spo11 occur at discrete sites with a sequence preference.
Murakami H, Nicolas A., Free PMC Article | 01/21/2010 |
| N-terminal part of Spo11p is a nuclear localization signal that is not specific for prophase I and is used to import proteins in vegetative yeast cells. | [Compartmentalization of Spo11p in vegetative cells of yeast Saccharomyces cerevisiae].
Komakhin RA, Komakhina VV. | 01/21/2010 |
| Analysis of mutation sites of isolated spo11-mutant alleles indicated that both N-terminal and C-terminal non-conserved residues of Spo11 are essential for the protein's function. | Both conserved and non-conserved regions of Spo11 are essential for meiotic recombination initiation in yeast.
Nag DK, Pata JD, Sironi M, Flood DR, Hart AM. | 01/21/2010 |
| Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114 occurs at the time of DNA double-stranded break formation. | Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114.
Sasanuma H, Murakami H, Fukuda T, Shibata T, Nicolas A, Ohta K., Free PMC Article | 01/21/2010 |
| mutation of a single gene (SIR2), which encodes a histone deacetylase, dramatically alters the genomic distribution of Spo11p-catalyzed DNA breaks | Loss of a histone deacetylase dramatically alters the genomic distribution of Spo11p-catalyzed DNA breaks in Saccharomyces cerevisiae.
Mieczkowski PA, Dominska M, Buck MJ, Lieb JD, Petes TD., Free PMC Article | 01/21/2010 |
| meiotic double-stranded breaks in budding yeast are processed by endonucleolytic cleavage that releases Spo11 attached to an oligonucleotide with a free 3'-OH | Endonucleolytic processing of covalent protein-linked DNA double-strand breaks.
Neale MJ, Pan J, Keeney S., Free PMC Article | 01/21/2010 |