| Yeast Kinesin-5 Motor Protein CIN8 Promotes Accurate Chromosome Segregation. | Yeast Kinesin-5 Motor Protein CIN8 Promotes Accurate Chromosome Segregation.
Sherwin D, Huetteman A, Wang Y., Free PMC Article | 08/13/2022 |
| Motility of Single Molecules and Clusters of Bi-directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells. | Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells.
Pandey H, Zvagelsky T, Popov M, Sadan M, Yanir N, Goldstein-Levitin A, Siegler N, Hershfinkel S, Abraham Y, Abraham R, Gheber LA, Gheber L. | 04/16/2022 |
| Characterization of the motility of monomeric kinesin-5/Cin8. | Characterization of the motility of monomeric kinesin-5/Cin8.
Yamagishi M, Maruyama Y, Sugawa M, Yajima J. | 07/3/2021 |
| These results suggest that Cin8-PP1 plays a critical role at kinetochores to promote accurate chromosome segregation by controlling Ndc80 attachment to microtubules. | A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation.
Suzuki A, Gupta A, Long SK, Evans R, Badger BL, Salmon ED, Biggins S, Bloom K., Free PMC Article | 10/19/2019 |
| Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement | Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement.
Fallesen T, Roostalu J, Duellberg C, Pruessner G, Surrey T., Free PMC Article | 12/2/2017 |
| Data suggest that approximately four Cin8 motor domains can bind each alpha/beta-tubulin dimer within microtubule lattice; Cin8 binds microtubules not only at the canonical site, but also one or more separate "non-canonical" sites. | The yeast kinesin-5 Cin8 interacts with the microtubule in a noncanonical manner.
Bell KM, Cha HK, Sindelar CV, Cochran JC., Free PMC Article | 09/30/2017 |
| The tail domain is one of the factors that regulate Cin8 directionality. | Deletion of the Tail Domain of the Kinesin-5 Cin8 Affects Its Directionality.
Düselder A, Fridman V, Thiede C, Wiesbaum A, Goldstein A, Klopfenstein DR, Zaitseva O, Janson ME, Gheber L, Schmidt CF., Free PMC Article | 10/31/2015 |
| Cin8 mutants lacking critical CDK phosphorylation sites suppress the requirement for Cdc14 and Cdc5 in anaphase spindle elongation. | FEAR-mediated activation of Cdc14 is the limiting step for spindle elongation and anaphase progression.
Roccuzzo M, Visintin C, Tili F, Visintin R. | 05/2/2015 |
| The deletion mutant cells exhibited reduced midzone-directed motility and efficiency to support spindle elongation, indicating the importance of directionality control for the anaphase function of Cin8. | Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry.
Gerson-Gurwitz A, Thiede C, Movshovich N, Fridman V, Podolskaya M, Danieli T, Lakämper S, Klopfenstein DR, Schmidt CF, Gheber L., Free PMC Article | 02/4/2012 |
| Dephosphorylation of Cin8 promotes its binding to the spindle microtubules before the onset of anaphase. | Phospho-regulation of kinesin-5 during anaphase spindle elongation.
Avunie-Masala R, Movshovich N, Nissenkorn Y, Gerson-Gurwitz A, Fridman V, Kõivomägi M, Loog M, Hoyt MA, Zaritsky A, Gheber L., Free PMC Article | 06/18/2011 |
| Cin8 can switch between two states of directional motility. On individual microtubules, Cin8 motors move toward the minus end, they switch to plus-end movement when part of a team of mechanically coupled motors cross-linking antiparallel microtubules. | Directional switching of the kinesin Cin8 through motor coupling.
Roostalu J, Hentrich C, Bieling P, Telley IA, Schiebel E, Surrey T. | 04/16/2011 |
| Data suggest that spindle disassembly is achieved by mechanistically distinct but functionally overlapping subprocesses, which are largely governed by the anaphase-promoting complex, Aurora B kinase, and kinesin-8. | Mitotic spindle disassembly occurs via distinct subprocesses driven by the anaphase-promoting complex, Aurora B kinase, and kinesin-8.
Woodruff JB, Drubin DG, Barnes G., Free PMC Article | 01/8/2011 |
| Clb2/Cdc28 drives spindle pole separation by direct phosphorylation of Kip1 and Cin8. | B-cyclin/CDKs regulate mitotic spindle assembly by phosphorylating kinesins-5 in budding yeast.
Chee MK, Haase SB., Free PMC Article | 09/6/2010 |
| Data observed that deletion of Cin8, a microtubule-crosslinking kinesin-5 motor protein, reduced the recovery rate in anaphase spindles, indicating that Cin8 contributes to the destabilization of iMT plus-ends. | Midzone organization restricts interpolar microtubule plus-end dynamics during spindle elongation.
Fridman V, Gerson-Gurwitz A, Movshovich N, Kupiec M, Gheber L., Free PMC Article | 01/21/2010 |
| Results show that the 2 micron plasmid of Saccharomyces cerevisiae uses the Kip1 motor, but not the functionally redundant Cin8 motor, for its precise nuclear localization and equal segregation. | The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation.
Cui H, Ghosh SK, Jayaram M., Free PMC Article | 01/21/2010 |
| Results suggest that dynein and Cin8 cooperate in anaphase midzone organization and influence microtubule dynamics, thus enabling progression through the slow phase of anaphase B. | Mid-anaphase arrest in S. cerevisiae cells eliminated for the function of Cin8 and dynein.
Gerson-Gurwitz A, Movshovich N, Avunie R, Fridman V, Moyal K, Katz B, Hoyt MA, Gheber L., Free PMC Article | 01/21/2010 |
| Study finds in budding yeast that the widely conserved kinesin-5 sliding motor proteins, Cin8p and Kip1p, mediate chromosome congression by suppressing kMT plus-end assembly of longer kinetochore microtubules. | Chromosome congression by Kinesin-5 motor-mediated disassembly of longer kinetochore microtubules.
Gardner MK, Bouck DC, Paliulis LV, Meehl JB, O'Toole ET, Haase J, Soubry A, Joglekar AP, Winey M, Salmon ED, Bloom K, Odde DJ., Free PMC Article | 01/21/2010 |
| Spindle elongation in anaphase in Saccharomyces cerevisiae cells mutated for the kinesin-5 motor protein Cin8 | Slk19-dependent mid-anaphase pause in kinesin-5-mutated cells.
Movshovich N, Fridman V, Gerson-Gurwitz A, Shumacher I, Gertsberg I, Fich A, Hoyt MA, Katz B, Gheber L. | 01/21/2010 |
| Ase1p and Cin8p affect nuclear positioning through astral micro-tubule-dependent mechanisms. | The spindle midzone microtubule-associated proteins Ase1p and Cin8p affect the number and orientation of astral microtubules in Saccharomyces cerevisiae.
de Gramont A, Barbour L, Ross KE, Cohen-Fix O. | 01/21/2010 |
| Results suggest that binding of microtubules is not sufficient for the functionality of Cin8p and that microtubule cross-linking by the tetrameric complex is essential for Cin8p mitotic functions. | Homotetrameric form of Cin8p, a Saccharomyces cerevisiae kinesin-5 motor, is essential for its in vivo function.
Hildebrandt ER, Gheber L, Kingsbury T, Hoyt MA. | 01/21/2010 |