| [URA3 affects artemisinic acid production by an engineered Saccharomyces cerevisiae in pilot-scale fermentation]. | [URA3 affects artemisinic acid production by an engineered Saccharomyces cerevisiae in pilot-scale fermentation].
Guo W, Ai L, Hu D, Chen Y, Geng M, Zheng L, Bai L. | 03/5/2022 |
| Repetitive delta-integration of a cellulase-encoding gene into the chromosome of an industrial Angel Yeast-derived strain by URA3 recycling. | Repetitive δ-integration of a cellulase-encoding gene into the chromosome of an industrial Angel Yeast-derived strain by URA3 recycling.
Zou S, Sun S, Zhang X, Li J, Guo J, Hong J, Ma Y, Zhang M. | 01/1/2022 |
| SIR2 Expression Noise Can Generate Heterogeneity in Viability but Does Not Affect Cell-to-Cell Epigenetic Silencing of Subtelomeric URA3 in Yeast. | SIR2 Expression Noise Can Generate Heterogeneity in Viability but Does Not Affect Cell-to-Cell Epigenetic Silencing of Subtelomeric URA3 in Yeast.
Liu J, Mosser L, Botanch C, François JM, Capp JP., Free PMC Article | 08/14/2021 |
| Role of the Carboxylate in Enzyme-Catalyzed Decarboxylation of Orotidine 5'-Monophosphate: Transition State Stabilization Dominates Over Ground State Destabilization. | Role of the Carboxylate in Enzyme-Catalyzed Decarboxylation of Orotidine 5'-Monophosphate: Transition State Stabilization Dominates Over Ground State Destabilization.
Goryanova B, Amyes TL, Richard JP., Free PMC Article | 10/3/2020 |
| most of the compensatory mutants were clustered in proximity to the catalytic center | Limits to Compensatory Mutations: Insights from Temperature-Sensitive Alleles.
Tomala K, Zrebiec P, Hartl DL., Free PMC Article | 01/18/2020 |
| Heritable silencing does not impact mutation rate but drives population size expansion and rapid epigenetic adaptation. This eventually leads to genetic assimilation of the silent phenotype by mutations that reduce or abolish URA3 expression. | Epigenetic gene silencing alters the mechanisms and rate of evolutionary adaptation.
Stajic D, Perfeito L, Jansen LET. | 06/1/2019 |
| we showed that high levels of copper (previously shown to elevate CUP1 transcription) lead to a substantial elevation in rate of both interhomolog and intra/sister chromatid recombination in the CUP1 array; recombination events that delete the URA3 insertion from the CUP1 array occur at a rate of >10(-3)/division in unselected cells. | Properties of Mitotic and Meiotic Recombination in the Tandemly-Repeated CUP1 Gene Cluster in the Yeast Saccharomyces cerevisiae.
Zhao Y, Dominska M, Petrova A, Bagshaw H, Kokoska RJ, Petes TD., Free PMC Article | 07/15/2017 |
| We found that mutations were scattered throughout the open reading frame of the URA3 gene but clustered to several hotspots with base substitutions/deletions at homo-polymeric runs. Surprisingly, we only detected two multiple mutants out of over 300 sequenced mutation events. | Microhomology-mediated end joining induces hypermutagenesis at breakpoint junctions.
Sinha S, Li F, Villarreal D, Shim JH, Yoon S, Myung K, Shim EY, Lee SE., Free PMC Article | 06/3/2017 |
| Analysis of a triple mutant cube shows small interactions between phosphodianion gripper side chains, which are consistent with steric crowding of the side chains around the phosphodianion at wild-type OMPDC | Enzyme architecture: deconstruction of the enzyme-activating phosphodianion interactions of orotidine 5'-monophosphate decarboxylase.
Goldman LM, Amyes TL, Goryanova B, Gerlt JA, Richard JP., Free PMC Article | 05/23/2015 |
| investigation of OMPDC/URA3 biocatalysis: substrate specificity; kinetics; conformational changes during substrate binding/decarboxylation | Catalysis by orotidine 5'-monophosphate decarboxylase: effect of 5-fluoro and 4'-substituents on the decarboxylation of two-part substrates.
Goryanova B, Spong K, Amyes TL, Richard JP., Free PMC Article | 03/16/2013 |
| Greater enthalpic transition state stabilization available from more extensive loop-substrate interactions for the S. cerevisiae-catalyzed reaction is largely balanced by a larger entropic requirement for immobilization of the larger loop at this enzyme. | An examination of the relationship between active site loop size and thermodynamic activation parameters for orotidine 5'-monophosphate decarboxylase from mesophilic and thermophilic organisms.
Toth K, Amyes TL, Wood BM, Chan KK, Gerlt JA, Richard JP., Free PMC Article | 01/21/2010 |
| the cytosolic face of the ER membrane serves as a "platform" for the degradation of Ura3p-CL1, which may also be the case for other CytoQC substrates | Degradation of a cytosolic protein requires endoplasmic reticulum-associated degradation machinery.
Metzger MB, Maurer MJ, Dancy BM, Michaelis S., Free PMC Article | 01/21/2010 |