Manufacture protocol Date 2/7/2007 OpArraysTM Protocol www.operon.com Page 1 Operon Homo sapiens 31K Operon Biotechnologies GmbH, BioCampus Cologne, Nattermannallee 1, 50829 Cologne, Germany When conducting your experiment, remove only the OpArrays slides you plan to use immediately and immediately return the remaining OpArrays to the pouch, leaving the desiccant pack in place. For added protection, OpArrays slides may be kept in their storage pouch in a desiccator. OpArrays Storage OpArrays microarrays are printed on epoxide slides, pre-processed and supplied ready-to-use. ° The microarray slides should be stored in their original container at room temperature in dry conditions (preferably desiccated) and protected from light. ° The slide box containing OpArrays is shipped in a re-sealable storage pouch containing desiccant which is ideal for continued storage. ° Both the storage pouches and slide storage boxes are manufactured from materials that minimize out-gassing and effectively block exposure to foreign contaminants that can elevate background levels in your experiments. ° When properly stored, OpArrays slides will remain hybridization competent for 4 months or more. Array Position on OpArrays Slides OpArrays slides are printed on the same side as the barcode (graphic to the right). The array area is 18mm wide, 54mm long, and is located 8mm from the top of the slide (barcode at bottom). The size and location are compatible with standard 22mm x 60mm cover slips and other equipment for handling microarrays printed on this substrate size. A list of previously tested equipment can be found in Appendix A. Technical Note: To reduce possible sources of experimental variation, which may effect experimental results, Operon strongly advises OpArrays users to gather completed OpArrays experimental data by scanning the OpArrays as soon as practical once the experiment is completed. Please schedule your experiments appropriately. Degradation of fluorescent dyes is reported post-hybridization; these effects are attributed to a variety of sources. There are several reports regarding reduction of Cy5 signal intensity relative to Cy3 due to the concentration of atmospheric ozone above 5 ppb in the atmosphere. The major consideration in preventing this effect seems to be in the transition from a wet to dry array surface. This protocol is designed to minimize these effects. Date 2/7/2007 OpArraysTM Protocol www.operon.com Page 2
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