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Platform GPL1058

Query DataSets for GPL1058

Status

Public on Mar 03, 2004

Title

NIAID - Mmbf

Technology type

spotted oligonucleotide

Distribution

non-commercial

Organism

Description

Printing by NIAID / Microarray Research Facility
Surface type: non-adsorptive, epoxy-coated glass
Attachment: covalent
Array description: The custom microarrays are manufactured by the Microarray Research Facility of the National Institute of Allergy and Infectious Diseases (NIH/NIAID). Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions (http://www.genomicsolutions.com ) that is fitted with a printhead and 48 SMP3 print tips from Telechem (http://www.arrayit.com ). The mouse microarray (Mmbf) probe set is based on 70mer oligonucleotides from Qiagen's Mouse Genome Oligo Set V2.0 (http://oligos.qiagen.com/arrays/omad.php ). A comprehensive gene list is also available at http://madb.niaid.nih.gov/
Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved to a normalized 25 micromolar concentration in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauroylsarcosine). The Mmbf microarray spot densities are 210 microns (center-to-center). The microarrays are printed on MWG Epoxy Slides (MWG Biotech Inc., # 1150-000000) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.

Submission date

Mar 01, 2004

Last update date

Dec 28, 2005

Contact name

Michael Wilson

E-mail(s)

mwilson@ambion.com

Phone

301-496-4287

URL

http://madb.niaid.nih.gov/

Organization name

National Institute of Allergy and Infectious Diseases

Department

Division of Intramural Research

Lab

Research Technologies Branch / Microarray Research Facility

Street address

50 South Drive, Room 5509

City

Bethesda

State/province

MD

ZIP/Postal code

20892

Country

USA

Data table header descriptions
ID	NIH mAdb well id plus replicate number
MADB_WELL_ID	NIH mAdb plate-well identifier for oligo sample
OLIGO_ID	Oligo identifier
GENE	Gene Name
UniGene	UniGene Identifier Build #Mm.134
DESCRIPTION	Gene Description
SPOT_ID	spot identifier

Data table
ID	MADB_WELL_ID	OLIGO_ID	GENE	UniGene	DESCRIPTION	SPOT_ID
1310604_1	1310604	MRF3_50-a12			Tamara prelabeled MRF3_50 Control_4	Tamara prelabeled MRF3_50 Control_4
1310628_1	1310628	MRF3_50-c12			Tamara prelabeled MRF3_50 Control_4	Tamara prelabeled MRF3_50 Control_4
1310652_1	1310652	MRF1_50-e12			MRF1_50 Control_4	MRF1_50 Control_4
1310676_1	1310676	MRF2_50-g12			MRF2_50 Control_4	MRF2_50 Control_4
1310598_1	1310598	AMB_6F			AMB_6F Control_3 Ambion spiked standard	AMB_6F Control_3 Ambion spiked standard
1310622_1	1310622	Alien_10			Alien_10 Control_3 Stratagene spiked standard	Alien_10 Control_3 Stratagene spiked standard
1310646_1	1310646	Empty/Unknown			EmptyWell	EmptyWell
1310670_1	1310670	Empty/Unknown			EmptyWell	EmptyWell
1195534_1	1195534	M200000106	Slc25a3	298	solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3	Slc25a3 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3
1195558_1	1195558	M200000130	Abca1	277376	ATP-binding cassette, sub-family A (ABC1), member 1	Abca1 ATP-binding cassette, sub-family A (ABC1), member 1
1195582_1	1195582	M200000154	Iapp	415	islet amyloid polypeptide	Iapp islet amyloid polypeptide
1195606_1	1195606	M200000178	Lamp2	486	lysosomal membrane glycoprotein 2	Lamp2 lysosomal membrane glycoprotein 2
1195528_1	1195528	M200000100	Guca2b	278	guanylate cyclase activator 2b (retina)	Guca2b guanylate cyclase activator 2b (retina)
1195552_1	1195552	M200000124	Cox5a	273403	cytochrome c oxidase, subunit Va	Cox5a cytochrome c oxidase, subunit Va
1195576_1	1195576	M200000148	Usp9x	242646	ubiquitin specific protease 9, X chromosome	Usp9x ubiquitin specific protease 9, X chromosome
1195600_1	1195600	M200000172	4921531G14Rik	244512	RIKEN cDNA 4921531G14 gene	4921531G14Rik RIKEN cDNA 4921531G14 gene
1195918_1	1195918	M200000486	Ccl3	1282	chemokine (C-C motif) ligand 3	Ccl3 chemokine (C-C motif) ligand 3
1195942_1	1195942	M200000510	Gck	220358	glucokinase	Gck glucokinase
1195966_1	1195966	M200000534	Trh	1363	thyrotropin releasing hormone	Trh thyrotropin releasing hormone
1195990_1	1195990	M200000558	Slpi	35215	secretory leukocyte protease inhibitor	Slpi secretory leukocyte protease inhibitor

Total number of rows: 18816

Table truncated, full table size 2367 Kbytes.

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML

Supplementary data files not provided

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