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Platform GPL14901 Query DataSets for GPL14901
Status Public on Nov 22, 2011
Title UCSF/Sil lab-Histoplasma capsulatum-G217Borfs2
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Histoplasma capsulatum
Manufacturer Sil Lab, UCSF
Manufacture protocol Spotted using DeRisi arrayer in Center for Advanced Technology, Genentech Hall, Mission Bay. 70-mer oligonucleotides representing each predicted gene are resuspended in 3XSSC and spotted on poly-lysine slides purchased from Erie Scientific.
Support glass
Coating poly-lysine
 
Description This is a revision of UCSF/Sil lab-Histoplasma capsulatum-G217Bprint2 (GEO accession GPL6125) adding 15 oligos relative to the original design. The new oligo IDs are prefixed with G217Borfs2. G217Borfs2.38n17 targets MS8 (GenBank AF292398) which is incorrectly merged with HISTO_ZT.Contig181-snap.97 in the predicted gene set, and the remaining 14 target novel transcripts identified during preliminary analysis of whole genome tiling array data (published with a revised and expanded set of novel transcripts in BMC Microbiol. 11:216).
 
Contributor(s) Foo CK, Sil A, Voorhies M, Inglis DO
Submission date Nov 17, 2011
Last update date Nov 22, 2011
Contact name Mark Voorhies
E-mail(s) mark.voorhies@ucsf.edu
Organization name University of California
Department Microbiology and Immunology
Lab Anita Sil
Street address 513 Parnassus, S472
City San Francisco
State/province CA
ZIP/Postal code 94143-0414
Country USA
 
Samples (80) GSM835834, GSM835835, GSM835836, GSM835837, GSM835838, GSM835839 
Series (6)
GSE33792 SRE1 Regulates Iron-Dependent Gene Expression in the Fungal Pathogen Histoplasma capsulatum
GSE45415 Comparative transcriptomics of infectious spores from the fungal pathogen Histoplasma capsulatum G217B
GSE45432 Comparative transcriptomics of infectious spores from the fungal pathogen Histoplasma capsulatum

Data table header descriptions
ID
Target Target or negative control name. Where the target is a predicted gene, names correspond to the predicted gene set from the Genome Sequencing Center (GSC) at Washington University. Numerical suffixes are offsets for the oligo relative to the target start, as reported by Array Oligo Selector. Targets based on whole genome tiling arrays are prefixed with WGTA.
SPOT_ID HistoBase
SEQUENCE
BLOCK Block on spotted array, corresponding to a single print tip.
ROW Row position within block.
COL Column position within block.

Data table
ID Target SPOT_ID SEQUENCE BLOCK ROW COL
G217Borfs1.10a1 HISTO_DM.Contig933.Fgenesh_Neurospora.175.final_new_72 G217Borfs1.10a1 GGTGGAAGAGGCTCGGTATCAAAGATCTTGTTTGGGTCGAGTACAAATGTTATAACAGGCAGCGCATTGA 4 5 17
G217Borfs1.10a10 HISTO_DM.Contig933.Fgenesh_Neurospora.33.final_new_98 G217Borfs1.10a10 CTCCGAAAATACCACCAGCCACTACGAAGGAGACATCACGATCCCTGTGGCGTTTCATATGCAGAGTAAC 8 5 18
G217Borfs1.10a11 HISTO_DM.Contig933.Fgenesh_Neurospora.33.final_new_89 G217Borfs1.10a11 AACGTGGAGCTCCGAAAATACCACCAGCCACTACGAAGGAGACATCACGATCCCTGTGGCGTTTCATATG 12 5 18
G217Borfs1.10a12 HISTO_DM.Contig933.Fgenesh_Neurospora.42.final_new_2690 G217Borfs1.10a12 TGCGGATCTTGTGTGCGAGCGCGCCAGACGTTATTTGAGTAGGTATTTTGACGTGACGCCTGAAACTAGT 16 5 18
G217Borfs1.10a13 HISTO_DM.Contig933.Fgenesh_Neurospora.6.final_new_63 G217Borfs1.10a13 TACGGCACGAGGCACCTTATGAGGCACATCAGGCATAGTACATACAAGTTCATAATATTTTCCCAACCGG 20 5 18
G217Borfs1.10a14 HISTO_DM.Contig933.Fgenesh_Neurospora.6.final_new_74 G217Borfs1.10a14 GCACCTTATGAGGCACATCAGGCATAGTACATACAAGTTCATAATATTTTCCCAACCGGTAATAGGCGGC 24 5 18
G217Borfs1.10a15 HISTO_DM.Contig933.Fgenesh_Neurospora.67.final_new_10 G217Borfs1.10a15 CAACGTCAGGAAATAAAGCCTCCCACTGCTGCCTTGGACGGATTGACCCCGTTAACGTTAAGGAGATGTA 28 5 18
G217Borfs1.10a16 HISTO_DM.Contig933.Fgenesh_Neurospora.67.final_new_3 G217Borfs1.10a16 TACAGATCAACGTCAGGAAATAAAGCCTCCCACTGCTGCCTTGGACGGATTGACCCCGTTAACGTTAAGG 32 5 18
G217Borfs1.10a17 HISTO_DM.Contig933.Fgenesh_Neurospora.8.final_new_101 G217Borfs1.10a17 TGCTCTCTTCAACGATTCGCCCGCCACAACCGCGCCACAGCCTCTGGCGTCCTCCCATCTTCAGGGCTCC 4 5 19
G217Borfs1.10a18 HISTO_DM.Contig933.Fgenesh_Neurospora.8.final_new_9 G217Borfs1.10a18 CGCCGCCTGGACGAGAGACCAATGTCCTGTACCAGCGGGGTCTCTCGGAATCAAGCTCTGCAGACTACAG 8 5 19
G217Borfs1.10a19 HISTO_DM.Contig933.Fgenesh_Neurospora.81.final_new_1578 G217Borfs1.10a19 GAGCGGGGCAAGCCATTGAGACATGTGGTATATTTAGAAGAAGGGATCAAGGAGTCGGTGGTAGAAGAGC 12 5 19
G217Borfs1.10a2 HISTO_DM.Contig933.Fgenesh_Neurospora.178.final_new_46 G217Borfs1.10a2 TTGTTTCGTTCTCGGGCGTGGATTCACTCGATGCCGATTTGTGGTTTCATCAATTTGTTTCATTGATATC 8 5 17
G217Borfs1.10a20 HISTO_DM.Contig933.Fgenesh_Neurospora.87.final_new_157 G217Borfs1.10a20 GAGCCAGCTCGCTGAAAATGGGCGCCGATAAACAAAATACTTTGGAAGAAGCGTGGTTGATCAGTAATAA 16 5 19
G217Borfs1.10a21 HISTO_DM.Contig933.Fgenesh_Neurospora.9.final_new_72 G217Borfs1.10a21 TGGTCCATCCAATTCAACCGATTGCGCAACACTCGTCGCGCCAAGGAGCCGGTAAAAAGAAGCCAACAGT 20 5 19
G217Borfs1.10a22 HISTO_DM.Contig933.Fgenesh_Neurospora.9.final_new_63 G217Borfs1.10a22 CACCCCACCTGGTCCATCCAATTCAACCGATTGCGCAACACTCGTCGCGCCAAGGAGCCGGTAAAAAGAA 24 5 19
G217Borfs1.10a23 HISTO_DM.Contig933.Fgenesh_Neurospora.94.final_new_246 G217Borfs1.10a23 AAGGATTCACCGTGTTCTGTCGATGAAAAAACTGGGTGGAGCGGCCGCAGTGGCGCCGTAACAAGAAGTA 28 5 19
G217Borfs1.10a24 HISTO_DM.Contig933.Fgenesh_Neurospora.99.final_new_61 G217Borfs1.10a24 CCGTGTGTGTTCTAGATAGGAAGACCTCCCGGCGAAACTCCGTTGTCAAACCCTCATATTTGATGGTCAA 32 5 19
G217Borfs1.10a3 HISTO_DM.Contig933.Fgenesh_Neurospora.178.final_new_55 G217Borfs1.10a3 TCTCGGGCGTGGATTCACTCGATGCCGATTTGTGGTTTCATCAATTTGTTTCATTGATATCGAGAACTCG 12 5 17
G217Borfs1.10a4 HISTO_DM.Contig933.Fgenesh_Neurospora.18.final_new_24 G217Borfs1.10a4 GCTGATGGGATTGTCGTATTCAAGCCCTTCGACGTTGCGTCACCTCAAGGTTGTATATCAAACGTTGGGC 16 5 17
G217Borfs1.10a5 HISTO_DM.Contig933.Fgenesh_Neurospora.182.final_new_769 G217Borfs1.10a5 TGACCGGTTCCCCCATCTTCAGAAAACTCGAAAGGCTACGGATGGTTGAGTTGCCTTATGCATCGCTACC 20 5 17

Total number of rows: 14976

Table truncated, full table size 2311 Kbytes.




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